Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS)
When considering the geographical expansion of marine toxins, the emergence of new toxins and the associated risk for human health, there is urgent need for versatile and efficient analytical methods that are able to detect a range, as wide as possible, of known or emerging toxins. Current detection...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-09-01
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| Series: | Toxins |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6651/10/9/375 |
| _version_ | 1811308113114955776 |
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| author | Inès Dom Ronel Biré Vincent Hort Gwenaëlle Lavison-Bompard Marina Nicolas Thierry Guérin |
| author_facet | Inès Dom Ronel Biré Vincent Hort Gwenaëlle Lavison-Bompard Marina Nicolas Thierry Guérin |
| author_sort | Inès Dom |
| collection | DOAJ |
| description | When considering the geographical expansion of marine toxins, the emergence of new toxins and the associated risk for human health, there is urgent need for versatile and efficient analytical methods that are able to detect a range, as wide as possible, of known or emerging toxins. Current detection methods for marine toxins rely on a priori defined target lists of toxins and are generally inappropriate for the detection and identification of emerging compounds. The authors describe the implementation of a recent approach for the non-targeted analysis of marine toxins in shellfish with a focus on a comprehensive workflow for the acquisition and treatment of the data generated after liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) analysis. First, the study was carried out in targeted mode to assess the performance of the method for known toxins with an extended range of polarities, including lipophilic toxins (okadaic acid, dinophysistoxins, azaspiracids, pectenotoxins, yessotoxins, cyclic imines, brevetoxins) and domoic acid. The targeted method, assessed for 14 toxins, shows good performance both in mussel and oyster extracts. The non-target potential of the method was then challenged via suspects and without a priori screening by blind analyzing mussel and oyster samples spiked with marine toxins. The data processing was optimized and successfully identified the toxins that were spiked in the blind samples. |
| first_indexed | 2024-04-13T09:16:20Z |
| format | Article |
| id | doaj.art-18ef1a8a713641d196b93ea8cd9cd053 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-13T09:16:20Z |
| publishDate | 2018-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-18ef1a8a713641d196b93ea8cd9cd0532022-12-22T02:52:43ZengMDPI AGToxins2072-66512018-09-0110937510.3390/toxins10090375toxins10090375Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS)Inès Dom0Ronel Biré1Vincent Hort2Gwenaëlle Lavison-Bompard3Marina Nicolas4Thierry Guérin5Laboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, FranceLaboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, FranceLaboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, FranceLaboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, FranceLaboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, FranceLaboratory for Food Safety, ANSES, Université Paris-Est, F-94701 Maisons-Alfort, FranceWhen considering the geographical expansion of marine toxins, the emergence of new toxins and the associated risk for human health, there is urgent need for versatile and efficient analytical methods that are able to detect a range, as wide as possible, of known or emerging toxins. Current detection methods for marine toxins rely on a priori defined target lists of toxins and are generally inappropriate for the detection and identification of emerging compounds. The authors describe the implementation of a recent approach for the non-targeted analysis of marine toxins in shellfish with a focus on a comprehensive workflow for the acquisition and treatment of the data generated after liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS) analysis. First, the study was carried out in targeted mode to assess the performance of the method for known toxins with an extended range of polarities, including lipophilic toxins (okadaic acid, dinophysistoxins, azaspiracids, pectenotoxins, yessotoxins, cyclic imines, brevetoxins) and domoic acid. The targeted method, assessed for 14 toxins, shows good performance both in mussel and oyster extracts. The non-target potential of the method was then challenged via suspects and without a priori screening by blind analyzing mussel and oyster samples spiked with marine toxins. The data processing was optimized and successfully identified the toxins that were spiked in the blind samples.http://www.mdpi.com/2072-6651/10/9/375marine toxinsLC-HRMStargeted analysissuspects screeningnon-targeted analysismethod characterization |
| spellingShingle | Inès Dom Ronel Biré Vincent Hort Gwenaëlle Lavison-Bompard Marina Nicolas Thierry Guérin Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS) Toxins marine toxins LC-HRMS targeted analysis suspects screening non-targeted analysis method characterization |
| title | Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS) |
| title_full | Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS) |
| title_fullStr | Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS) |
| title_full_unstemmed | Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS) |
| title_short | Extended Targeted and Non-Targeted Strategies for the Analysis of Marine Toxins in Mussels and Oysters by (LC-HRMS) |
| title_sort | extended targeted and non targeted strategies for the analysis of marine toxins in mussels and oysters by lc hrms |
| topic | marine toxins LC-HRMS targeted analysis suspects screening non-targeted analysis method characterization |
| url | http://www.mdpi.com/2072-6651/10/9/375 |
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