The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop
Neural progenitors undergo temporal patterning to generate diverse neurons in a chronological order. This process is well-studied in the developing Drosophila brain and conserved in mammals. During larval stages, intermediate neural progenitors (INPs) serially express Dichaete (D), grainyhead (Grh)...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-07-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/46566 |
| _version_ | 1811201708287590400 |
|---|---|
| author | Merve Deniz Abdusselamoglu Elif Eroglu Thomas R Burkard Jürgen A Knoblich |
| author_facet | Merve Deniz Abdusselamoglu Elif Eroglu Thomas R Burkard Jürgen A Knoblich |
| author_sort | Merve Deniz Abdusselamoglu |
| collection | DOAJ |
| description | Neural progenitors undergo temporal patterning to generate diverse neurons in a chronological order. This process is well-studied in the developing Drosophila brain and conserved in mammals. During larval stages, intermediate neural progenitors (INPs) serially express Dichaete (D), grainyhead (Grh) and eyeless (Ey/Pax6), but how the transitions are regulated is not precisely understood. Here, we developed a method to isolate transcriptomes of INPs in their distinct temporal states to identify a complete set of temporal patterning factors. Our analysis identifies odd-paired (opa), as a key regulator of temporal patterning. Temporal patterning is initiated when the SWI/SNF complex component Osa induces D and its repressor Opa at the same time but with distinct kinetics. Then, high Opa levels repress D to allow Grh transcription and progress to the next temporal state. We propose that Osa and its target genes opa and D form an incoherent feedforward loop (FFL) and a new mechanism allowing the successive expression of temporal identities. |
| first_indexed | 2024-04-12T02:25:12Z |
| format | Article |
| id | doaj.art-18ef728abe594f30859d6a106e43ef31 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:25:12Z |
| publishDate | 2019-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-18ef728abe594f30859d6a106e43ef312022-12-22T03:52:00ZengeLife Sciences Publications LtdeLife2050-084X2019-07-01810.7554/eLife.46566The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loopMerve Deniz Abdusselamoglu0https://orcid.org/0000-0001-8467-3947Elif Eroglu1Thomas R Burkard2Jürgen A Knoblich3https://orcid.org/0000-0002-6751-3404IMBA – Institute of Molecular Biotechnology of the Austrian Academy of Science, Vienna Biocenter (VBC), Vienna, AustriaIMBA – Institute of Molecular Biotechnology of the Austrian Academy of Science, Vienna Biocenter (VBC), Vienna, AustriaIMBA – Institute of Molecular Biotechnology of the Austrian Academy of Science, Vienna Biocenter (VBC), Vienna, AustriaIMBA – Institute of Molecular Biotechnology of the Austrian Academy of Science, Vienna Biocenter (VBC), Vienna, AustriaNeural progenitors undergo temporal patterning to generate diverse neurons in a chronological order. This process is well-studied in the developing Drosophila brain and conserved in mammals. During larval stages, intermediate neural progenitors (INPs) serially express Dichaete (D), grainyhead (Grh) and eyeless (Ey/Pax6), but how the transitions are regulated is not precisely understood. Here, we developed a method to isolate transcriptomes of INPs in their distinct temporal states to identify a complete set of temporal patterning factors. Our analysis identifies odd-paired (opa), as a key regulator of temporal patterning. Temporal patterning is initiated when the SWI/SNF complex component Osa induces D and its repressor Opa at the same time but with distinct kinetics. Then, high Opa levels repress D to allow Grh transcription and progress to the next temporal state. We propose that Osa and its target genes opa and D form an incoherent feedforward loop (FFL) and a new mechanism allowing the successive expression of temporal identities.https://elifesciences.org/articles/46566temporal identityneural progenitortranscription factors |
| spellingShingle | Merve Deniz Abdusselamoglu Elif Eroglu Thomas R Burkard Jürgen A Knoblich The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop eLife temporal identity neural progenitor transcription factors |
| title | The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop |
| title_full | The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop |
| title_fullStr | The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop |
| title_full_unstemmed | The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop |
| title_short | The transcription factor odd-paired regulates temporal identity in transit-amplifying neural progenitors via an incoherent feed-forward loop |
| title_sort | transcription factor odd paired regulates temporal identity in transit amplifying neural progenitors via an incoherent feed forward loop |
| topic | temporal identity neural progenitor transcription factors |
| url | https://elifesciences.org/articles/46566 |
| work_keys_str_mv | AT mervedenizabdusselamoglu thetranscriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT eliferoglu thetranscriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT thomasrburkard thetranscriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT jurgenaknoblich thetranscriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT mervedenizabdusselamoglu transcriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT eliferoglu transcriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT thomasrburkard transcriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop AT jurgenaknoblich transcriptionfactoroddpairedregulatestemporalidentityintransitamplifyingneuralprogenitorsviaanincoherentfeedforwardloop |