Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects
Abstract Background Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes with their dense layer towards the soft tissue and their porous layer towards...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-06-01
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| Series: | International Journal of Implant Dentistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40729-021-00333-y |
| _version_ | 1818744007975501824 |
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| author | Balazs Feher Karol Ali Apaza Alccayhuaman Franz Josef Strauss Jung-Seok Lee Stefan Tangl Ulrike Kuchler Reinhard Gruber |
| author_facet | Balazs Feher Karol Ali Apaza Alccayhuaman Franz Josef Strauss Jung-Seok Lee Stefan Tangl Ulrike Kuchler Reinhard Gruber |
| author_sort | Balazs Feher |
| collection | DOAJ |
| description | Abstract Background Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes with their dense layer towards the soft tissue and their porous layer towards the bony defect area. However, evidence supporting this recommendation is lacking. This study aimed to determine whether the alignment of bilayer collagen membranes has an effect on bone regeneration. Methods In two groups of ten male Sprague-Dawley rats each, a 5-mm calvarial defect was created. Thereafter, the defect was randomly covered with a bilayer, resorbable, pure type I and III collagen membrane placed either regularly or upside-down (i.e., dense layer towards bone defect). After 4 weeks of healing, micro-computed tomography (μCT), histology, and histomorphometry of the inner cylindrical region of interest (4.5 mm in diameter) were performed to assess new bone formation and the consolidation of the collagen membrane in the defect area. Results Quantitative μCT showed similar bone volume (median 8.0 mm3, interquartile range 7.0–10.0 vs. 6.2 mm3, 4.3–9.4, p = 0.06) and trabecular thickness (0.21 mm, 0.19–0.23 vs. 0.18 mm, 0.17–0.20, p = 0.03) between upside-down and regular placement, both leading to an almost complete bony coverage. Histomorphometry showed comparable new bone areas between the upside-down and regularly placed membranes, 3.9 mm2 (2.7–5.4) vs. 3.8 mm2 (2.2–4.0, p = 0.31), respectively. Both treatment groups revealed the same regeneration patterns and spatial distribution of bone with and without collagen fibers, as well as residual collagen fibers. Conclusions Our data support the osteoconductive properties of collagen membranes and suggest that bone regeneration is facilitated regardless of membrane layer alignment. |
| first_indexed | 2024-12-18T02:37:28Z |
| format | Article |
| id | doaj.art-18f315ab57f94b238b0ec54376870f97 |
| institution | Directory Open Access Journal |
| issn | 2198-4034 |
| language | English |
| last_indexed | 2024-12-18T02:37:28Z |
| publishDate | 2021-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | International Journal of Implant Dentistry |
| spelling | doaj.art-18f315ab57f94b238b0ec54376870f972022-12-21T21:23:45ZengSpringerOpenInternational Journal of Implant Dentistry2198-40342021-06-017111010.1186/s40729-021-00333-yOsteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defectsBalazs Feher0Karol Ali Apaza Alccayhuaman1Franz Josef Strauss2Jung-Seok Lee3Stefan Tangl4Ulrike Kuchler5Reinhard Gruber6Department of Oral Biology, University Clinic of Dentistry, Medical University of ViennaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of ViennaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of ViennaDepartment of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry, Yonsei UniversityCore Facility Hard Tissue and Biomaterial Research, Karl Donath Laboratory, University Clinic of Dentistry, Medical University of ViennaDepartment of Oral Surgery, University Clinic of Dentistry, Medical University of ViennaDepartment of Oral Biology, University Clinic of Dentistry, Medical University of ViennaAbstract Background Bilayer collagen membranes are routinely used in guided bone/tissue regeneration to serve as osteoconductive scaffolds and prevent the invasion of soft tissues. It is recommended to place the membranes with their dense layer towards the soft tissue and their porous layer towards the bony defect area. However, evidence supporting this recommendation is lacking. This study aimed to determine whether the alignment of bilayer collagen membranes has an effect on bone regeneration. Methods In two groups of ten male Sprague-Dawley rats each, a 5-mm calvarial defect was created. Thereafter, the defect was randomly covered with a bilayer, resorbable, pure type I and III collagen membrane placed either regularly or upside-down (i.e., dense layer towards bone defect). After 4 weeks of healing, micro-computed tomography (μCT), histology, and histomorphometry of the inner cylindrical region of interest (4.5 mm in diameter) were performed to assess new bone formation and the consolidation of the collagen membrane in the defect area. Results Quantitative μCT showed similar bone volume (median 8.0 mm3, interquartile range 7.0–10.0 vs. 6.2 mm3, 4.3–9.4, p = 0.06) and trabecular thickness (0.21 mm, 0.19–0.23 vs. 0.18 mm, 0.17–0.20, p = 0.03) between upside-down and regular placement, both leading to an almost complete bony coverage. Histomorphometry showed comparable new bone areas between the upside-down and regularly placed membranes, 3.9 mm2 (2.7–5.4) vs. 3.8 mm2 (2.2–4.0, p = 0.31), respectively. Both treatment groups revealed the same regeneration patterns and spatial distribution of bone with and without collagen fibers, as well as residual collagen fibers. Conclusions Our data support the osteoconductive properties of collagen membranes and suggest that bone regeneration is facilitated regardless of membrane layer alignment.https://doi.org/10.1186/s40729-021-00333-yCollagen membranesGBRGTRMicro-computed tomographyHistologyHistomorphometry |
| spellingShingle | Balazs Feher Karol Ali Apaza Alccayhuaman Franz Josef Strauss Jung-Seok Lee Stefan Tangl Ulrike Kuchler Reinhard Gruber Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects International Journal of Implant Dentistry Collagen membranes GBR GTR Micro-computed tomography Histology Histomorphometry |
| title | Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects |
| title_full | Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects |
| title_fullStr | Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects |
| title_full_unstemmed | Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects |
| title_short | Osteoconductive properties of upside-down bilayer collagen membranes in rat calvarial defects |
| title_sort | osteoconductive properties of upside down bilayer collagen membranes in rat calvarial defects |
| topic | Collagen membranes GBR GTR Micro-computed tomography Histology Histomorphometry |
| url | https://doi.org/10.1186/s40729-021-00333-y |
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