Multiple virus sorting based on aptamer-modified microspheres in a TSAW device
Abstract Due to the overlapping epidemiology and clinical manifestations of flaviviruses, differential diagnosis of these viral diseases is complicated, and the results are unreliable. There is perpetual demand for a simplified, sensitive, rapid and inexpensive assay with less cross-reactivity. The...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2023-05-01
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| Series: | Microsystems & Nanoengineering |
| Online Access: | https://doi.org/10.1038/s41378-023-00523-1 |
| _version_ | 1797822783674122240 |
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| author | Xianglian Liu Xuan Chen Yangchao Dong Chuanyu Zhang Xiaoli Qu Yingfeng Lei Zhuangde Jiang Xueyong Wei |
| author_facet | Xianglian Liu Xuan Chen Yangchao Dong Chuanyu Zhang Xiaoli Qu Yingfeng Lei Zhuangde Jiang Xueyong Wei |
| author_sort | Xianglian Liu |
| collection | DOAJ |
| description | Abstract Due to the overlapping epidemiology and clinical manifestations of flaviviruses, differential diagnosis of these viral diseases is complicated, and the results are unreliable. There is perpetual demand for a simplified, sensitive, rapid and inexpensive assay with less cross-reactivity. The ability to sort distinct virus particles from a mixture of biological samples is crucial for improving the sensitivity of diagnoses. Therefore, we developed a sorting system for the subsequent differential diagnosis of dengue and tick-borne encephalitis in the early stage. We employed aptamer-modified polystyrene (PS) microspheres with different diameters to specifically capture dengue virus (DENV) and tick-borne encephalitis virus (TBEV), and utilized a traveling surface acoustic wave (TSAW) device to accomplish microsphere sorting according to particle size. The captured viruses were then characterized by laser scanning confocal microscopy (LSCM), field emission scanning electron microscopy (FE-SEM) and reverse transcription-polymerase chain reaction (RT‒PCR). The characterization results indicated that the acoustic sorting process was effective and damage-free for subsequent analysis. Furthermore, the strategy can be utilized for sample pretreatment in the differential diagnosis of viral diseases. |
| first_indexed | 2024-03-13T10:14:17Z |
| format | Article |
| id | doaj.art-18f7e4eaa74b4000be3605e418e59ab6 |
| institution | Directory Open Access Journal |
| issn | 2055-7434 |
| language | English |
| last_indexed | 2024-03-13T10:14:17Z |
| publishDate | 2023-05-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Microsystems & Nanoengineering |
| spelling | doaj.art-18f7e4eaa74b4000be3605e418e59ab62023-05-21T11:19:02ZengNature Publishing GroupMicrosystems & Nanoengineering2055-74342023-05-019111010.1038/s41378-023-00523-1Multiple virus sorting based on aptamer-modified microspheres in a TSAW deviceXianglian Liu0Xuan Chen1Yangchao Dong2Chuanyu Zhang3Xiaoli Qu4Yingfeng Lei5Zhuangde Jiang6Xueyong Wei7State Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong UniversityState Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong UniversityDepartment of Microbiology, the Fourth Military Medical UniversityState Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong UniversityState Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong UniversityDepartment of Microbiology, the Fourth Military Medical UniversityState Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong UniversityState Key Laboratory for Manufacturing Systems Engineering, Xi’an Jiaotong UniversityAbstract Due to the overlapping epidemiology and clinical manifestations of flaviviruses, differential diagnosis of these viral diseases is complicated, and the results are unreliable. There is perpetual demand for a simplified, sensitive, rapid and inexpensive assay with less cross-reactivity. The ability to sort distinct virus particles from a mixture of biological samples is crucial for improving the sensitivity of diagnoses. Therefore, we developed a sorting system for the subsequent differential diagnosis of dengue and tick-borne encephalitis in the early stage. We employed aptamer-modified polystyrene (PS) microspheres with different diameters to specifically capture dengue virus (DENV) and tick-borne encephalitis virus (TBEV), and utilized a traveling surface acoustic wave (TSAW) device to accomplish microsphere sorting according to particle size. The captured viruses were then characterized by laser scanning confocal microscopy (LSCM), field emission scanning electron microscopy (FE-SEM) and reverse transcription-polymerase chain reaction (RT‒PCR). The characterization results indicated that the acoustic sorting process was effective and damage-free for subsequent analysis. Furthermore, the strategy can be utilized for sample pretreatment in the differential diagnosis of viral diseases.https://doi.org/10.1038/s41378-023-00523-1 |
| spellingShingle | Xianglian Liu Xuan Chen Yangchao Dong Chuanyu Zhang Xiaoli Qu Yingfeng Lei Zhuangde Jiang Xueyong Wei Multiple virus sorting based on aptamer-modified microspheres in a TSAW device Microsystems & Nanoengineering |
| title | Multiple virus sorting based on aptamer-modified microspheres in a TSAW device |
| title_full | Multiple virus sorting based on aptamer-modified microspheres in a TSAW device |
| title_fullStr | Multiple virus sorting based on aptamer-modified microspheres in a TSAW device |
| title_full_unstemmed | Multiple virus sorting based on aptamer-modified microspheres in a TSAW device |
| title_short | Multiple virus sorting based on aptamer-modified microspheres in a TSAW device |
| title_sort | multiple virus sorting based on aptamer modified microspheres in a tsaw device |
| url | https://doi.org/10.1038/s41378-023-00523-1 |
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