Functional Characterization of Muscarinic Receptors in Human Schwann Cells
Functional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both in central and peripheral nervous system. Rat Schwann cells (SCs) express different muscarinic receptor subtypes with the prevalence of the M2 subtype. The selective stimulation of...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/18/6666 |
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| author | Roberta Piovesana Alessandro Faroni Ada Maria Tata Adam J. Reid |
| author_facet | Roberta Piovesana Alessandro Faroni Ada Maria Tata Adam J. Reid |
| author_sort | Roberta Piovesana |
| collection | DOAJ |
| description | Functional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both in central and peripheral nervous system. Rat Schwann cells (SCs) express different muscarinic receptor subtypes with the prevalence of the M2 subtype. The selective stimulation of this receptor subtype inhibits SC proliferation, improving their differentiation towards myelinating phenotype. In this work, we describe for the first time that human SCs are cholinoceptive as they express several muscarinic receptor subtypes and, as for rat SCs, M2 receptor is one of the most abundant. Human SCs, isolated from adult nerves, were cultured in vitro and stimulated with M2 muscarinic agonist arecaidine propargyl ester (APE). Similarly to that observed in rat, M2 receptor activation causes a decreased cell proliferation and promotes SC differentiation as suggested by increased Egr2 expression with an improved spindle-like shape cell morphology. Conversely, the non-selective stimulation of muscarinic receptors appears to promote cell proliferation with a reduction of SC average cell diameter. The data obtained demonstrate that human SCs are cholinoceptive and that human cultured SCs may represent an interesting tool to understand their physiology and increase the knowledge on how the cholinergic stimulation may contribute to address human SC development in normal and pathological conditions. |
| first_indexed | 2024-03-10T16:23:12Z |
| format | Article |
| id | doaj.art-18fa51d0f95a435a8f40ad974b9f31a0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T16:23:12Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-18fa51d0f95a435a8f40ad974b9f31a02023-11-20T13:27:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-09-012118666610.3390/ijms21186666Functional Characterization of Muscarinic Receptors in Human Schwann CellsRoberta Piovesana0Alessandro Faroni1Ada Maria Tata2Adam J. Reid3Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, ItalyBlond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PT, UKDepartment of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, P.le Aldo Moro, 5, 00185 Rome, ItalyBlond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PT, UKFunctional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both in central and peripheral nervous system. Rat Schwann cells (SCs) express different muscarinic receptor subtypes with the prevalence of the M2 subtype. The selective stimulation of this receptor subtype inhibits SC proliferation, improving their differentiation towards myelinating phenotype. In this work, we describe for the first time that human SCs are cholinoceptive as they express several muscarinic receptor subtypes and, as for rat SCs, M2 receptor is one of the most abundant. Human SCs, isolated from adult nerves, were cultured in vitro and stimulated with M2 muscarinic agonist arecaidine propargyl ester (APE). Similarly to that observed in rat, M2 receptor activation causes a decreased cell proliferation and promotes SC differentiation as suggested by increased Egr2 expression with an improved spindle-like shape cell morphology. Conversely, the non-selective stimulation of muscarinic receptors appears to promote cell proliferation with a reduction of SC average cell diameter. The data obtained demonstrate that human SCs are cholinoceptive and that human cultured SCs may represent an interesting tool to understand their physiology and increase the knowledge on how the cholinergic stimulation may contribute to address human SC development in normal and pathological conditions.https://www.mdpi.com/1422-0067/21/18/6666human Schwann cellsacetylcholinemuscarinic receptorsdifferentiationglial cells |
| spellingShingle | Roberta Piovesana Alessandro Faroni Ada Maria Tata Adam J. Reid Functional Characterization of Muscarinic Receptors in Human Schwann Cells International Journal of Molecular Sciences human Schwann cells acetylcholine muscarinic receptors differentiation glial cells |
| title | Functional Characterization of Muscarinic Receptors in Human Schwann Cells |
| title_full | Functional Characterization of Muscarinic Receptors in Human Schwann Cells |
| title_fullStr | Functional Characterization of Muscarinic Receptors in Human Schwann Cells |
| title_full_unstemmed | Functional Characterization of Muscarinic Receptors in Human Schwann Cells |
| title_short | Functional Characterization of Muscarinic Receptors in Human Schwann Cells |
| title_sort | functional characterization of muscarinic receptors in human schwann cells |
| topic | human Schwann cells acetylcholine muscarinic receptors differentiation glial cells |
| url | https://www.mdpi.com/1422-0067/21/18/6666 |
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