A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi.
Abstract Objective: To study the frequency and diversity of fungi involved in onychomycosis. Method: The cross-sectional study was conducted from February 2018 to February 2019 at the Department of Microbiology, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, an...
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| Format: | Article |
| Language: | English |
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Pakistan Medical Association
2020-10-01
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| Series: | Journal of the Pakistan Medical Association |
| Online Access: | https://www.ojs.jpma.org.pk/index.php/public_html/article/view/1811 |
| _version_ | 1797820557858701312 |
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| author | Raziuddin Ahmed Bahram Khan Khoso Fakhur Uddin Nida Rehman Muhammad Sohail Saleem Hafiz |
| author_facet | Raziuddin Ahmed Bahram Khan Khoso Fakhur Uddin Nida Rehman Muhammad Sohail Saleem Hafiz |
| author_sort | Raziuddin Ahmed |
| collection | DOAJ |
| description |
Abstract
Objective: To study the frequency and diversity of fungi involved in onychomycosis.
Method: The cross-sectional study was conducted from February 2018 to February 2019 at the Department of Microbiology, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, and the University of Karachi. Specimens were taken from patients suffering with onychomycosis attending the Dermatology Department of Jinnah Postgraduate Medical Centre and were processed for the isolation of fungi. Specimens were processed for the diagnosis by potassium hydroxide mount for microscopic evaluation and mycological culture to determine the type of fungus involved in onychomycosis. Fungi were isolated using Sabouraud Dextrose Agar with antibiotics cycloheximide and chloramphenicol, and without antibiotics. Plates were observed for the growth periodically until 4th week. Appearance of any colony was studied further for identification on the basis of cultural characteristics and microscopy. Precise identification of Candida species was done.
Results: Of the 230 samples, 85(36.96%) were from males and 145(63.04%) from females. Potassium hydroxide mount showed fungal element in 134(58.26%) specimens, while in 111(48.26%) patients the fungal culture was positive. Nine (3.9%) cases were culture-positive but potassium hydroxide-negative, while among the 134(58.26%) potassium hydroxide-positive cases, 32(23.9%) were culture-negative.
Key Words: Candida, Dermatophytes, Fungal infections of nail, KOH mount, Non-dermatophyte molds, Onychomycosis, Tinea unguium.
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| first_indexed | 2024-03-13T09:40:09Z |
| format | Article |
| id | doaj.art-18fac3db8d194c34ab919716dc209546 |
| institution | Directory Open Access Journal |
| issn | 0030-9982 |
| language | English |
| last_indexed | 2024-03-13T09:40:09Z |
| publishDate | 2020-10-01 |
| publisher | Pakistan Medical Association |
| record_format | Article |
| series | Journal of the Pakistan Medical Association |
| spelling | doaj.art-18fac3db8d194c34ab919716dc2095462023-05-25T04:25:16ZengPakistan Medical AssociationJournal of the Pakistan Medical Association0030-99822020-10-0110.47391/JPMA.390A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. Raziuddin Ahmed 0Bahram Khan Khoso 1Fakhur Uddin 2Nida Rehman 3Muhammad Sohail 4Saleem Hafiz5Department of Pathology, SAHARA Medical College, Karachi, PakistanDepartment of Dermatology, Jinnah Postgraduate Medical Centre, Jinnah Sind Medical University, Karachi, PakistanDepartment of Microbiology, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, PakistanDepartment of Microbiology, University of Karachi, PakistanDepartment of Microbiology, University of Karachi, PakistanDepartment of Microbiology, Sind Institute of Urology and Transplant, Dow Medical College and Civil Hospital Karachi, Pakistan Abstract Objective: To study the frequency and diversity of fungi involved in onychomycosis. Method: The cross-sectional study was conducted from February 2018 to February 2019 at the Department of Microbiology, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi, and the University of Karachi. Specimens were taken from patients suffering with onychomycosis attending the Dermatology Department of Jinnah Postgraduate Medical Centre and were processed for the isolation of fungi. Specimens were processed for the diagnosis by potassium hydroxide mount for microscopic evaluation and mycological culture to determine the type of fungus involved in onychomycosis. Fungi were isolated using Sabouraud Dextrose Agar with antibiotics cycloheximide and chloramphenicol, and without antibiotics. Plates were observed for the growth periodically until 4th week. Appearance of any colony was studied further for identification on the basis of cultural characteristics and microscopy. Precise identification of Candida species was done. Results: Of the 230 samples, 85(36.96%) were from males and 145(63.04%) from females. Potassium hydroxide mount showed fungal element in 134(58.26%) specimens, while in 111(48.26%) patients the fungal culture was positive. Nine (3.9%) cases were culture-positive but potassium hydroxide-negative, while among the 134(58.26%) potassium hydroxide-positive cases, 32(23.9%) were culture-negative. Key Words: Candida, Dermatophytes, Fungal infections of nail, KOH mount, Non-dermatophyte molds, Onychomycosis, Tinea unguium. Continuous... https://www.ojs.jpma.org.pk/index.php/public_html/article/view/1811 |
| spellingShingle | Raziuddin Ahmed Bahram Khan Khoso Fakhur Uddin Nida Rehman Muhammad Sohail Saleem Hafiz A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. Journal of the Pakistan Medical Association |
| title | A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. |
| title_full | A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. |
| title_fullStr | A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. |
| title_full_unstemmed | A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. |
| title_short | A cross sectional study to observe the diversity of fungal species in Onychomycosis isolated from a tertiary care hospital in Karachi. |
| title_sort | cross sectional study to observe the diversity of fungal species in onychomycosis isolated from a tertiary care hospital in karachi |
| url | https://www.ojs.jpma.org.pk/index.php/public_html/article/view/1811 |
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