<i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i>
Very-long-chain alkane plays an important role as an aliphatic barrier. We previously reported that <i>BnCER1-2</i> was responsible for alkane biosynthesis in <i>Brassica napus</i> and improved plant tolerance to drought. However, how the expression of <i>BnCER1-2</i...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/5/4287 |
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| author | Saiyu Wang Chengcheng Bai Na Luo Youwei Jiang Yulu Wang Yu Liu Chunjie Chen Yuxin Wang Qiaoqiao Gan Shurong Jin Yu Ni |
| author_facet | Saiyu Wang Chengcheng Bai Na Luo Youwei Jiang Yulu Wang Yu Liu Chunjie Chen Yuxin Wang Qiaoqiao Gan Shurong Jin Yu Ni |
| author_sort | Saiyu Wang |
| collection | DOAJ |
| description | Very-long-chain alkane plays an important role as an aliphatic barrier. We previously reported that <i>BnCER1-2</i> was responsible for alkane biosynthesis in <i>Brassica napus</i> and improved plant tolerance to drought. However, how the expression of <i>BnCER1-2</i> is regulated is still unknown. Through yeast one-hybrid screening, we identified a transcriptional regulator of <i>BnCER1-2</i>, BnaC9.DEWAX1, which encodes AP2\ERF transcription factor. BnaC9.DEWAX1 targets the nucleus and displays transcriptional repression activity. Electrophoretic mobility shift and transient transcriptional assays suggested that BnaC9.DEWAX1 repressed the transcription of <i>BnCER1-2</i> by directly interacting with its promoter. <i>BnaC9.DEWAX1</i> was expressed predominantly in leaves and siliques, which was similar to the expression pattern of <i>BnCER1-2</i>. Hormone and major abiotic stresses such as drought and high salinity affected the expression of <i>BnaC9.DEWAX1</i>. Ectopic expression of <i>BnaC9.DEWAX1</i> in Arabidopsis plants down-regulated <i>CER1</i> transcription levels and resulted in a reduction in alkanes and total wax loads in leaves and stems when compared with the wild type, whereas the wax depositions in the <i>dewax</i> mutant returned to the wild type level after complementation of <i>BnaC9.DEWAX1</i> in the mutant. Moreover, both altered cuticular wax composition and structure contribute to increased epidermal permeability in <i>BnaC9.DEWAX1</i> overexpression lines. Collectively, these results support the notion that BnaC9.DEWAX1 negatively regulates wax biosynthesis by binding directly to the <i>BnCER1-2</i> promoter, which provides insights into the regulatory mechanism of wax biosynthesis in <i>B. napus</i>. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:23:15Z |
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| spelling | doaj.art-18fba10de51e40d1ab42f2a1fbe812e32023-11-17T07:46:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245428710.3390/ijms24054287<i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i>Saiyu Wang0Chengcheng Bai1Na Luo2Youwei Jiang3Yulu Wang4Yu Liu5Chunjie Chen6Yuxin Wang7Qiaoqiao Gan8Shurong Jin9Yu Ni10College of Agronomy, Qingdao Agriculture University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agriculture University, Qingdao 266109, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, ChinaCollege of Agronomy, Qingdao Agriculture University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agriculture University, Qingdao 266109, ChinaCollege of Agronomy, Qingdao Agriculture University, Qingdao 266109, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, ChinaCollege of Agronomy and Biotechnology, Southwest University, Chongqing 400716, ChinaCollege of Agronomy, Qingdao Agriculture University, Qingdao 266109, ChinaVery-long-chain alkane plays an important role as an aliphatic barrier. We previously reported that <i>BnCER1-2</i> was responsible for alkane biosynthesis in <i>Brassica napus</i> and improved plant tolerance to drought. However, how the expression of <i>BnCER1-2</i> is regulated is still unknown. Through yeast one-hybrid screening, we identified a transcriptional regulator of <i>BnCER1-2</i>, BnaC9.DEWAX1, which encodes AP2\ERF transcription factor. BnaC9.DEWAX1 targets the nucleus and displays transcriptional repression activity. Electrophoretic mobility shift and transient transcriptional assays suggested that BnaC9.DEWAX1 repressed the transcription of <i>BnCER1-2</i> by directly interacting with its promoter. <i>BnaC9.DEWAX1</i> was expressed predominantly in leaves and siliques, which was similar to the expression pattern of <i>BnCER1-2</i>. Hormone and major abiotic stresses such as drought and high salinity affected the expression of <i>BnaC9.DEWAX1</i>. Ectopic expression of <i>BnaC9.DEWAX1</i> in Arabidopsis plants down-regulated <i>CER1</i> transcription levels and resulted in a reduction in alkanes and total wax loads in leaves and stems when compared with the wild type, whereas the wax depositions in the <i>dewax</i> mutant returned to the wild type level after complementation of <i>BnaC9.DEWAX1</i> in the mutant. Moreover, both altered cuticular wax composition and structure contribute to increased epidermal permeability in <i>BnaC9.DEWAX1</i> overexpression lines. Collectively, these results support the notion that BnaC9.DEWAX1 negatively regulates wax biosynthesis by binding directly to the <i>BnCER1-2</i> promoter, which provides insights into the regulatory mechanism of wax biosynthesis in <i>B. napus</i>.https://www.mdpi.com/1422-0067/24/5/4287<i>Brassica napus</i>cuticular wax biosynthesis<i>BnCER1-2</i>BnaC9.DEWAX1transcription repressor |
| spellingShingle | Saiyu Wang Chengcheng Bai Na Luo Youwei Jiang Yulu Wang Yu Liu Chunjie Chen Yuxin Wang Qiaoqiao Gan Shurong Jin Yu Ni <i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i> International Journal of Molecular Sciences <i>Brassica napus</i> cuticular wax biosynthesis <i>BnCER1-2</i> BnaC9.DEWAX1 transcription repressor |
| title | <i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i> |
| title_full | <i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i> |
| title_fullStr | <i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i> |
| title_full_unstemmed | <i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i> |
| title_short | <i>Brassica napus</i> BnaC9.DEWAX1 Negatively Regulates Wax Biosynthesis via Transcriptional Suppression of <i>BnCER1-2</i> |
| title_sort | i brassica napus i bnac9 dewax1 negatively regulates wax biosynthesis via transcriptional suppression of i bncer1 2 i |
| topic | <i>Brassica napus</i> cuticular wax biosynthesis <i>BnCER1-2</i> BnaC9.DEWAX1 transcription repressor |
| url | https://www.mdpi.com/1422-0067/24/5/4287 |
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