Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice
Mitochondrial dysfunction is widely reported in various diseases and contributes to their pathogenesis. We assessed the effect of cocoa flavanols supplementation on mitochondrial function and whole metabolism, and we explored whether the mitochondrial deacetylase sirtuin-3 (Sirt3) is involved or not...
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MDPI AG
2021-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/10/3466 |
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| author | Frédéric Nicolas Daussin Alexane Cuillerier Julianne Touron Samir Bensaid Bruno Melo Ali Al Rewashdy Goutham Vasam Keir J. Menzies Mary-Ellen Harper Elsa Heyman Yan Burelle |
| author_facet | Frédéric Nicolas Daussin Alexane Cuillerier Julianne Touron Samir Bensaid Bruno Melo Ali Al Rewashdy Goutham Vasam Keir J. Menzies Mary-Ellen Harper Elsa Heyman Yan Burelle |
| author_sort | Frédéric Nicolas Daussin |
| collection | DOAJ |
| description | Mitochondrial dysfunction is widely reported in various diseases and contributes to their pathogenesis. We assessed the effect of cocoa flavanols supplementation on mitochondrial function and whole metabolism, and we explored whether the mitochondrial deacetylase sirtuin-3 (Sirt3) is involved or not. We explored the effects of 15 days of CF supplementation in wild type and Sirt3<sup>-/-</sup> mice. Whole-body metabolism was assessed by indirect calorimetry, and an oral glucose tolerance test was performed to assess glucose metabolism. Mitochondrial respiratory function was assessed in permeabilised fibres and the pyridine nucleotides content (NAD<sup>+</sup> and NADH) were quantified. In the wild type, CF supplementation significantly modified whole-body metabolism by promoting carbohydrate use and improved glucose tolerance. CF supplementation induced a significant increase of mitochondrial mass, while significant qualitative adaptation occurred to maintain H<sub>2</sub>O<sub>2</sub> production and cellular oxidative stress. CF supplementation induced a significant increase in NAD<sup>+</sup> and NADH content. All the effects mentioned above were blunted in Sirt3<sup>-/-</sup> mice. Collectively, CF supplementation boosted the NAD metabolism that stimulates sirtuins metabolism and improved mitochondrial function, which likely contributed to the observed whole-body metabolism adaptation, with a greater ability to use carbohydrates, at least partially through Sirt3. |
| first_indexed | 2024-03-10T06:17:54Z |
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| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T06:17:54Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-18fdb95ae54b483cb0b5a160643ac53d2023-11-22T19:28:59ZengMDPI AGNutrients2072-66432021-09-011310346610.3390/nu13103466Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy MiceFrédéric Nicolas Daussin0Alexane Cuillerier1Julianne Touron2Samir Bensaid3Bruno Melo4Ali Al Rewashdy5Goutham Vasam6Keir J. Menzies7Mary-Ellen Harper8Elsa Heyman9Yan Burelle10ULR 7369—URePSSS—Unité de Recherche Pluridisciplinaire Sport Santé Société, University Lille, University Artois, University Littoral Côte d’Opale, F-59000 Lille, FranceInterdisciplinary School of Health Sciences and Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, CanadaINRAE, UMR1019, Unité de Nutrition Humaine (UNH), Équipe ASMS, Université Clermont Auvergne, 63001 Clermont-Ferrand, FranceULR 7369—URePSSS—Unité de Recherche Pluridisciplinaire Sport Santé Société, University Lille, University Artois, University Littoral Côte d’Opale, F-59000 Lille, FranceDepartment of Physical Education, Exercise Physiology Laboratory, Federal University of Minas Gerais, Belo Horizonte, MG 31270-901, BrazilInterdisciplinary School of Health Sciences and Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, CanadaInterdisciplinary School of Health Sciences and Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, CanadaInterdisciplinary School of Health Sciences and Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, CanadaDepartment of Biochemistry Microbiology and Immunology, Faculty of Medicine, Ottawa Institute of Systems Biology, University of Ottawa, Ottawa, ON K1H 8M5, CanadaULR 7369—URePSSS—Unité de Recherche Pluridisciplinaire Sport Santé Société, University Lille, University Artois, University Littoral Côte d’Opale, F-59000 Lille, FranceInterdisciplinary School of Health Sciences and Faculty of Health Sciences, University of Ottawa, Ottawa, ON K1H 8M5, CanadaMitochondrial dysfunction is widely reported in various diseases and contributes to their pathogenesis. We assessed the effect of cocoa flavanols supplementation on mitochondrial function and whole metabolism, and we explored whether the mitochondrial deacetylase sirtuin-3 (Sirt3) is involved or not. We explored the effects of 15 days of CF supplementation in wild type and Sirt3<sup>-/-</sup> mice. Whole-body metabolism was assessed by indirect calorimetry, and an oral glucose tolerance test was performed to assess glucose metabolism. Mitochondrial respiratory function was assessed in permeabilised fibres and the pyridine nucleotides content (NAD<sup>+</sup> and NADH) were quantified. In the wild type, CF supplementation significantly modified whole-body metabolism by promoting carbohydrate use and improved glucose tolerance. CF supplementation induced a significant increase of mitochondrial mass, while significant qualitative adaptation occurred to maintain H<sub>2</sub>O<sub>2</sub> production and cellular oxidative stress. CF supplementation induced a significant increase in NAD<sup>+</sup> and NADH content. All the effects mentioned above were blunted in Sirt3<sup>-/-</sup> mice. Collectively, CF supplementation boosted the NAD metabolism that stimulates sirtuins metabolism and improved mitochondrial function, which likely contributed to the observed whole-body metabolism adaptation, with a greater ability to use carbohydrates, at least partially through Sirt3.https://www.mdpi.com/2072-6643/13/10/3466cocoa flavanolsNAD metabolismmitochondrial massglucose metabolismskeletal muscle |
| spellingShingle | Frédéric Nicolas Daussin Alexane Cuillerier Julianne Touron Samir Bensaid Bruno Melo Ali Al Rewashdy Goutham Vasam Keir J. Menzies Mary-Ellen Harper Elsa Heyman Yan Burelle Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice Nutrients cocoa flavanols NAD metabolism mitochondrial mass glucose metabolism skeletal muscle |
| title | Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice |
| title_full | Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice |
| title_fullStr | Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice |
| title_full_unstemmed | Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice |
| title_short | Dietary Cocoa Flavanols Enhance Mitochondrial Function in Skeletal Muscle and Modify Whole-Body Metabolism in Healthy Mice |
| title_sort | dietary cocoa flavanols enhance mitochondrial function in skeletal muscle and modify whole body metabolism in healthy mice |
| topic | cocoa flavanols NAD metabolism mitochondrial mass glucose metabolism skeletal muscle |
| url | https://www.mdpi.com/2072-6643/13/10/3466 |
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