Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells
Abstract Background Schwannoma arising from peripheral nervous sheaths is a benign tumor. Methods To evaluate cell cytotoxicity, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction and terminal deoxynucleotidyltransferase UTP nick-end labeling (TUNEL) assays were use...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-018-0693-x |
| _version_ | 1818518886196183040 |
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| author | Eun Jung Sohn Kyoung-mi Bak Yun-kyeong Nam Hwan Tae Park |
| author_facet | Eun Jung Sohn Kyoung-mi Bak Yun-kyeong Nam Hwan Tae Park |
| author_sort | Eun Jung Sohn |
| collection | DOAJ |
| description | Abstract Background Schwannoma arising from peripheral nervous sheaths is a benign tumor. Methods To evaluate cell cytotoxicity, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction and terminal deoxynucleotidyltransferase UTP nick-end labeling (TUNEL) assays were used. A microRNA (miRNA) array was used to identify the miRNAs involved in curcumin-induced apoptosis. To examine miRNA expression, quantitative RT-PCR was used. Results In this study, curcumin exerted cellular cytotoxicity against RT4 schwannoma cells, with an increase in TUNEL-positive cells. Curcumin also activated the expression of apoptotic proteins, such as polyADP ribose polymerase, caspase-3, and caspase-9. The miRNA array revealed that seven miRNAs (miRNA 350, miRNA 17-2-3p, let 7e-3p, miRNA1224, miRNA 466b-1-3p, miRNA 18a-5p, and miRNA 322-5p) were downregulated following treatment with both 10 and 20 μM curcumin in RT4 cells, while four miRNAs (miRNA122-5p, miRNA 3473, miRNA182, and miRNA344a-3p) were upregulated. Interestingly, transfection with a miRNA 344a-3p mimic downregulated the mRNA expression of Bcl2 and upregulated that of Bax, Curcumin treatment in RT 4 cells also reduced the mRNA expression of Bcl2 and enhanced expression of Bax, Overexpression of miRNA344a-3p mimic combined with curcumin treatment activated the expression of apoptotic proteins, including procaspase-9 and cleaved caspase-3 while inhibition of miRNA 344a-3p using miR344a-3p inhibitor repressed cleaved caspase-3 and -9 in curcumin treated RT-4 cells compared to control. Conclusions Our findings demonstrate that curcumin induces apoptosis in schwannoma cells via miRNA 344a-3p. Thus, curcumin may serve as a potent therapeutic agent for the treatment of schwannoma. |
| first_indexed | 2024-12-11T01:16:24Z |
| format | Article |
| id | doaj.art-190574cbda4f4a1a80a6ea5771e3bba2 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-11T01:16:24Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-190574cbda4f4a1a80a6ea5771e3bba22022-12-22T01:25:52ZengBMCCancer Cell International1475-28672018-12-0118111010.1186/s12935-018-0693-xUpregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cellsEun Jung Sohn0Kyoung-mi Bak1Yun-kyeong Nam2Hwan Tae Park3Peripheral Neuropathy Research Center, Department of Molecular Neuroscience, College of Medicine, Dong-A UniversityPeripheral Neuropathy Research Center, Department of Molecular Neuroscience, College of Medicine, Dong-A UniversityPeripheral Neuropathy Research Center, Department of Molecular Neuroscience, College of Medicine, Dong-A UniversityPeripheral Neuropathy Research Center, Department of Molecular Neuroscience, College of Medicine, Dong-A UniversityAbstract Background Schwannoma arising from peripheral nervous sheaths is a benign tumor. Methods To evaluate cell cytotoxicity, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction and terminal deoxynucleotidyltransferase UTP nick-end labeling (TUNEL) assays were used. A microRNA (miRNA) array was used to identify the miRNAs involved in curcumin-induced apoptosis. To examine miRNA expression, quantitative RT-PCR was used. Results In this study, curcumin exerted cellular cytotoxicity against RT4 schwannoma cells, with an increase in TUNEL-positive cells. Curcumin also activated the expression of apoptotic proteins, such as polyADP ribose polymerase, caspase-3, and caspase-9. The miRNA array revealed that seven miRNAs (miRNA 350, miRNA 17-2-3p, let 7e-3p, miRNA1224, miRNA 466b-1-3p, miRNA 18a-5p, and miRNA 322-5p) were downregulated following treatment with both 10 and 20 μM curcumin in RT4 cells, while four miRNAs (miRNA122-5p, miRNA 3473, miRNA182, and miRNA344a-3p) were upregulated. Interestingly, transfection with a miRNA 344a-3p mimic downregulated the mRNA expression of Bcl2 and upregulated that of Bax, Curcumin treatment in RT 4 cells also reduced the mRNA expression of Bcl2 and enhanced expression of Bax, Overexpression of miRNA344a-3p mimic combined with curcumin treatment activated the expression of apoptotic proteins, including procaspase-9 and cleaved caspase-3 while inhibition of miRNA 344a-3p using miR344a-3p inhibitor repressed cleaved caspase-3 and -9 in curcumin treated RT-4 cells compared to control. Conclusions Our findings demonstrate that curcumin induces apoptosis in schwannoma cells via miRNA 344a-3p. Thus, curcumin may serve as a potent therapeutic agent for the treatment of schwannoma.http://link.springer.com/article/10.1186/s12935-018-0693-xCurcuminMicroRNASchwannomaRT4 |
| spellingShingle | Eun Jung Sohn Kyoung-mi Bak Yun-kyeong Nam Hwan Tae Park Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells Cancer Cell International Curcumin MicroRNA Schwannoma RT4 |
| title | Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells |
| title_full | Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells |
| title_fullStr | Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells |
| title_full_unstemmed | Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells |
| title_short | Upregulation of microRNA 344a-3p is involved in curcumin induced apoptosis in RT4 schwannoma cells |
| title_sort | upregulation of microrna 344a 3p is involved in curcumin induced apoptosis in rt4 schwannoma cells |
| topic | Curcumin MicroRNA Schwannoma RT4 |
| url | http://link.springer.com/article/10.1186/s12935-018-0693-x |
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