First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.)
Leaf spot symptoms were spotted in two olive orchards in Istria and in Kvarner Gulf, Croatia. Fungal species from three representative isolates (P13 LECIII, R18 BI, JA20 NP) have been morphologically characterized based on the colony and conidial characteristics. Several techniques were performed fo...
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MDPI AG
2023-09-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/10/1067 |
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| author | Elena Petrović Karolina Vrandečić Jasenka Ćosić Edyta Đermić Sara Godena |
| author_facet | Elena Petrović Karolina Vrandečić Jasenka Ćosić Edyta Đermić Sara Godena |
| author_sort | Elena Petrović |
| collection | DOAJ |
| description | Leaf spot symptoms were spotted in two olive orchards in Istria and in Kvarner Gulf, Croatia. Fungal species from three representative isolates (P13 LECIII, R18 BI, JA20 NP) have been morphologically characterized based on the colony and conidial characteristics. Several techniques were performed for inducing the sporulation of the JA20 NP isolate. Only PDA + banana medium was successful. PCR was conducted for ITS, TUB, and EF1α gene regions. Phylogenetic analyses were performed using internal transcribed spacer, beta-tubulin, and translation elongation factor 1-alpha sequence data. Three types of tests were conducted: a pathogenicity test on detached leaves, on detached and scratched leaves, and on olive seedlings. Ultimately, from the morphological characterizations, DNA sequence analysis of ITS, TUB, and EF1α gene regions, and phylogenetic analysis, these species were identified as <i>Nigrospora gorlenkoana</i> Novobr., <i>Nigrospora osmanthi</i> Mei Wang & L. Cai, and <i>Nigrospora philosophiae-doctoris</i> M. Raza, Qian Chen & L. Cai. This is the first report of <i>Nigrospora</i> species causing leaf spot on olive trees and the first report of <i>Nigrospora philosophiae-doctoris</i> as a plant pathogen. Fungal leaf diseases in conditions that are favorable for infection and disease development can lead to a decrease in the yield and olive oil quality. Therefore, it is necessary to conduct further research and the monitoring of fungal leaf diseases. |
| first_indexed | 2024-03-10T21:12:27Z |
| format | Article |
| id | doaj.art-1905dea1807342d0861ba92d586d5524 |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T21:12:27Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-1905dea1807342d0861ba92d586d55242023-11-19T16:38:53ZengMDPI AGHorticulturae2311-75242023-09-01910106710.3390/horticulturae9101067First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.)Elena Petrović0Karolina Vrandečić1Jasenka Ćosić2Edyta Đermić3Sara Godena4Institute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, CroatiaFaculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, CroatiaFaculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, 31000 Osijek, CroatiaFaculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, CroatiaInstitute of Agriculture and Tourism, Karla Huguesa 8, 52440 Poreč, CroatiaLeaf spot symptoms were spotted in two olive orchards in Istria and in Kvarner Gulf, Croatia. Fungal species from three representative isolates (P13 LECIII, R18 BI, JA20 NP) have been morphologically characterized based on the colony and conidial characteristics. Several techniques were performed for inducing the sporulation of the JA20 NP isolate. Only PDA + banana medium was successful. PCR was conducted for ITS, TUB, and EF1α gene regions. Phylogenetic analyses were performed using internal transcribed spacer, beta-tubulin, and translation elongation factor 1-alpha sequence data. Three types of tests were conducted: a pathogenicity test on detached leaves, on detached and scratched leaves, and on olive seedlings. Ultimately, from the morphological characterizations, DNA sequence analysis of ITS, TUB, and EF1α gene regions, and phylogenetic analysis, these species were identified as <i>Nigrospora gorlenkoana</i> Novobr., <i>Nigrospora osmanthi</i> Mei Wang & L. Cai, and <i>Nigrospora philosophiae-doctoris</i> M. Raza, Qian Chen & L. Cai. This is the first report of <i>Nigrospora</i> species causing leaf spot on olive trees and the first report of <i>Nigrospora philosophiae-doctoris</i> as a plant pathogen. Fungal leaf diseases in conditions that are favorable for infection and disease development can lead to a decrease in the yield and olive oil quality. Therefore, it is necessary to conduct further research and the monitoring of fungal leaf diseases.https://www.mdpi.com/2311-7524/9/10/1067fungal diseaseisolateolive treepathogenicity |
| spellingShingle | Elena Petrović Karolina Vrandečić Jasenka Ćosić Edyta Đermić Sara Godena First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.) Horticulturae fungal disease isolate olive tree pathogenicity |
| title | First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.) |
| title_full | First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.) |
| title_fullStr | First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.) |
| title_full_unstemmed | First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.) |
| title_short | First Report of <i>Nigrospora</i> Species Causing Leaf Spot on Olive (<i>Olea europaea</i> L.) |
| title_sort | first report of i nigrospora i species causing leaf spot on olive i olea europaea i l |
| topic | fungal disease isolate olive tree pathogenicity |
| url | https://www.mdpi.com/2311-7524/9/10/1067 |
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