Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice
Ziyuan Li,1,* Yu Gan,1,* Ting Kang,1,* Yi Zhao,1 Tianguang Huang,2 Yuhao Chen,3 Jin Liu,1 Bowen Ke1 1Department of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-03-01
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| Series: | Journal of Pain Research |
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| Online Access: | https://www.dovepress.com/camphor-attenuates-hyperalgesia-in-neuropathic-pain-models-in-mice-peer-reviewed-fulltext-article-JPR |
| _version_ | 1811154243195764736 |
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| author | Li Z Gan Y Kang T Zhao Y Huang T Chen Y Liu J Ke B |
| author_facet | Li Z Gan Y Kang T Zhao Y Huang T Chen Y Liu J Ke B |
| author_sort | Li Z |
| collection | DOAJ |
| description | Ziyuan Li,1,* Yu Gan,1,* Ting Kang,1,* Yi Zhao,1 Tianguang Huang,2 Yuhao Chen,3 Jin Liu,1 Bowen Ke1 1Department of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China; 2Frontiers Science Center for Disease-Related Molecular Network, Sichuan University West China Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China; 3West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bowen Ke, Department of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu, Sichuan, People’s Republic of China, Tel +86-28-85422114, Fax +86-28-85582944, Email bowenke@scu.edu.cnBackground: The treatment of neuropathic pain is still a major troublesome clinical problem. The existing therapeutic drugs have limited analgesic effect and obvious adverse reactions, which presents opportunities and challenges for the development of new analgesic drugs. Camphor, a kind of monoterpene, has been shown anti-inflammatory and analgesic effects in traditional Chinese medicine. But we know little about its effect in neuropathic pain. In this article, We have verified the reliable analgesic effect of camphor in the neuropathic pain model caused by different predispositions.Methods: The nociceptive response of mice was induced by transient receptor potential A1 (TRPA1) agonist to verify the effect of camphor on the nociceptive response. Multiple paclitaxel (PTX) injection models, Single oxaliplatin (OXA) injection models, Chronic constriction injury (CCI) models and Streptozotocin-induced (STZ) diabetic neuropathic pain models were used in this study. We verified the analgesic effect of camphor in mice by acetone test and conditioned place aversion test. At the same time, comparing the adverse reaction of nervous system between camphor and pregabalin at equivalent dose in locomotor activity test and rotarod test. Using patch clamp to verify the effect of camphor on dorsal root ganglion (DRG) excitability.Results: In behavioral test, compared with vehicle group, camphor significantly reduced the spontaneous nociception caused by TRPA1 agonist-formalina and allyl isothiocyanate (AITC). Compared with vehicle group, camphor significantly reduced the flinching and licking time in neuropathic pain model mice, including PTX, OXA, STZ and CCI induced peripheral neuralgia models. Compared with vehicle group, pregabalin significantly increased the resting time and reduced the average speed without resting and distance in locomotor activity test, reduced the time stayed on rotarod in rotarod test. In patch clamp test, compared with vehicle group, camphor significantly reduced the action potential (AP) firing frequency of DRG.Conclusion: Camphor can alleviate the symptoms of hyperalgesia in various neuropathic pain models, and has no obvious adverse reactions compared with pregabalin. This effect is related to the down-regulation of DRG neuron excitability.Keywords: camphor, neuropathic pain, TRPA1, hyperalgesia |
| first_indexed | 2024-04-10T04:12:23Z |
| format | Article |
| id | doaj.art-19072221df204303a2556c3b19092737 |
| institution | Directory Open Access Journal |
| issn | 1178-7090 |
| language | English |
| last_indexed | 2024-04-10T04:12:23Z |
| publishDate | 2023-03-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Pain Research |
| spelling | doaj.art-19072221df204303a2556c3b190927372023-03-12T15:18:22ZengDove Medical PressJournal of Pain Research1178-70902023-03-01Volume 1678579582169Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in MiceLi ZGan YKang TZhao YHuang TChen YLiu JKe BZiyuan Li,1,* Yu Gan,1,* Ting Kang,1,* Yi Zhao,1 Tianguang Huang,2 Yuhao Chen,3 Jin Liu,1 Bowen Ke1 1Department of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China; 2Frontiers Science Center for Disease-Related Molecular Network, Sichuan University West China Hospital, Sichuan University, Chengdu, Sichuan, People’s Republic of China; 3West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bowen Ke, Department of Anesthesiology, Laboratory of Anesthesia and Critical Care Medicine, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu, Sichuan, People’s Republic of China, Tel +86-28-85422114, Fax +86-28-85582944, Email bowenke@scu.edu.cnBackground: The treatment of neuropathic pain is still a major troublesome clinical problem. The existing therapeutic drugs have limited analgesic effect and obvious adverse reactions, which presents opportunities and challenges for the development of new analgesic drugs. Camphor, a kind of monoterpene, has been shown anti-inflammatory and analgesic effects in traditional Chinese medicine. But we know little about its effect in neuropathic pain. In this article, We have verified the reliable analgesic effect of camphor in the neuropathic pain model caused by different predispositions.Methods: The nociceptive response of mice was induced by transient receptor potential A1 (TRPA1) agonist to verify the effect of camphor on the nociceptive response. Multiple paclitaxel (PTX) injection models, Single oxaliplatin (OXA) injection models, Chronic constriction injury (CCI) models and Streptozotocin-induced (STZ) diabetic neuropathic pain models were used in this study. We verified the analgesic effect of camphor in mice by acetone test and conditioned place aversion test. At the same time, comparing the adverse reaction of nervous system between camphor and pregabalin at equivalent dose in locomotor activity test and rotarod test. Using patch clamp to verify the effect of camphor on dorsal root ganglion (DRG) excitability.Results: In behavioral test, compared with vehicle group, camphor significantly reduced the spontaneous nociception caused by TRPA1 agonist-formalina and allyl isothiocyanate (AITC). Compared with vehicle group, camphor significantly reduced the flinching and licking time in neuropathic pain model mice, including PTX, OXA, STZ and CCI induced peripheral neuralgia models. Compared with vehicle group, pregabalin significantly increased the resting time and reduced the average speed without resting and distance in locomotor activity test, reduced the time stayed on rotarod in rotarod test. In patch clamp test, compared with vehicle group, camphor significantly reduced the action potential (AP) firing frequency of DRG.Conclusion: Camphor can alleviate the symptoms of hyperalgesia in various neuropathic pain models, and has no obvious adverse reactions compared with pregabalin. This effect is related to the down-regulation of DRG neuron excitability.Keywords: camphor, neuropathic pain, TRPA1, hyperalgesiahttps://www.dovepress.com/camphor-attenuates-hyperalgesia-in-neuropathic-pain-models-in-mice-peer-reviewed-fulltext-article-JPRcamphorneuropathic paintrpa1hyperalgesia |
| spellingShingle | Li Z Gan Y Kang T Zhao Y Huang T Chen Y Liu J Ke B Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice Journal of Pain Research camphor neuropathic pain trpa1 hyperalgesia |
| title | Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice |
| title_full | Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice |
| title_fullStr | Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice |
| title_full_unstemmed | Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice |
| title_short | Camphor Attenuates Hyperalgesia in Neuropathic Pain Models in Mice |
| title_sort | camphor attenuates hyperalgesia in neuropathic pain models in mice |
| topic | camphor neuropathic pain trpa1 hyperalgesia |
| url | https://www.dovepress.com/camphor-attenuates-hyperalgesia-in-neuropathic-pain-models-in-mice-peer-reviewed-fulltext-article-JPR |
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