A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.
Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the ges...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24905832/pdf/?tool=EBI |
| _version_ | 1819180952992088064 |
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| author | Carlos Salomon Maria Jose Torres Miharu Kobayashi Katherin Scholz-Romero Luis Sobrevia Aneta Dobierzewska Sebastian E Illanes Murray D Mitchell Gregory E Rice |
| author_facet | Carlos Salomon Maria Jose Torres Miharu Kobayashi Katherin Scholz-Romero Luis Sobrevia Aneta Dobierzewska Sebastian E Illanes Murray D Mitchell Gregory E Rice |
| author_sort | Carlos Salomon |
| collection | DOAJ |
| description | Studies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the gestational-age release profile and bioactivity of placental cell-derived exosome in maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant and pregnant women in the first (FT, 6-12 weeks), second (ST, 22-24 weeks) and third (TT, 32-38 weeks) trimester. The number of exosomes and placental exosome contribution were determined by quantifying immunoreactive exosomal CD63 and placenta-specific marker (PLAP), respectively. The effect of exosomes isolated from FT, ST and TT on endothelial cell migration were established using a real-time, live-cell imaging system (Incucyte). Exosome plasma concentration was more than 50-fold greater in pregnant women than in non-pregnant women (p<0.001). During normal healthy pregnancy, the number of exosomes present in maternal plasma increased significantly with gestational age by more that two-fold (p<0.001). Exosomes isolated from FT, ST and TT increased endothelial cell migration by 1.9±0.1, 1.6±0.2 and 1.3±0.1-fold, respectively compared to the control. Pregnancy is associated with a dramatic increase in the number of exosomes present in plasma and maternal plasma exosomes are bioactive. While the role of placental cell-derived exosome in regulating maternal and/or fetal vascular responses remains to be elucidated, changes in exosome profile may be of clinical utility in the diagnosis of placental dysfunction. |
| first_indexed | 2024-12-22T22:22:31Z |
| format | Article |
| id | doaj.art-1909cfc02a3c4c76a5ebc22e35d21d00 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T22:22:31Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1909cfc02a3c4c76a5ebc22e35d21d002022-12-21T18:10:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0196e9866710.1371/journal.pone.0098667A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration.Carlos SalomonMaria Jose TorresMiharu KobayashiKatherin Scholz-RomeroLuis SobreviaAneta DobierzewskaSebastian E IllanesMurray D MitchellGregory E RiceStudies completed to date provide persuasive evidence that placental cell-derived exosomes play a significant role in intercellular communication pathways that potentially contribute to placentation and development of materno-fetal vascular circulation. The aim of this study was to establish the gestational-age release profile and bioactivity of placental cell-derived exosome in maternal plasma. Plasma samples (n = 20 per pregnant group) were obtained from non-pregnant and pregnant women in the first (FT, 6-12 weeks), second (ST, 22-24 weeks) and third (TT, 32-38 weeks) trimester. The number of exosomes and placental exosome contribution were determined by quantifying immunoreactive exosomal CD63 and placenta-specific marker (PLAP), respectively. The effect of exosomes isolated from FT, ST and TT on endothelial cell migration were established using a real-time, live-cell imaging system (Incucyte). Exosome plasma concentration was more than 50-fold greater in pregnant women than in non-pregnant women (p<0.001). During normal healthy pregnancy, the number of exosomes present in maternal plasma increased significantly with gestational age by more that two-fold (p<0.001). Exosomes isolated from FT, ST and TT increased endothelial cell migration by 1.9±0.1, 1.6±0.2 and 1.3±0.1-fold, respectively compared to the control. Pregnancy is associated with a dramatic increase in the number of exosomes present in plasma and maternal plasma exosomes are bioactive. While the role of placental cell-derived exosome in regulating maternal and/or fetal vascular responses remains to be elucidated, changes in exosome profile may be of clinical utility in the diagnosis of placental dysfunction.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24905832/pdf/?tool=EBI |
| spellingShingle | Carlos Salomon Maria Jose Torres Miharu Kobayashi Katherin Scholz-Romero Luis Sobrevia Aneta Dobierzewska Sebastian E Illanes Murray D Mitchell Gregory E Rice A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. PLoS ONE |
| title | A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_full | A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_fullStr | A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_full_unstemmed | A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_short | A gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration. |
| title_sort | gestational profile of placental exosomes in maternal plasma and their effects on endothelial cell migration |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24905832/pdf/?tool=EBI |
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