Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection
Abstract Background Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30–50% of CAP during peak years. An early and rapid diagnostic method is key for guiding clinicians in their choice of antibiotics. Methods The rec...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-019-4750-4 |
| _version_ | 1819168994146385920 |
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| author | Guanhua Xue Shaoli Li Hanqing Zhao Chao Yan Yanling Feng Jinghua Cui Tingting Jiang Jing Yuan |
| author_facet | Guanhua Xue Shaoli Li Hanqing Zhao Chao Yan Yanling Feng Jinghua Cui Tingting Jiang Jing Yuan |
| author_sort | Guanhua Xue |
| collection | DOAJ |
| description | Abstract Background Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30–50% of CAP during peak years. An early and rapid diagnostic method is key for guiding clinicians in their choice of antibiotics. Methods The recombinase-aided amplification (RAA) assay is a recently developed, rapid detection method that has been used for the detection of several pathogens. The assays were performed in a one-step single tube reaction at 39° Celsius within 15–30 min. In this study, we established an RAA assay for M. pneumoniae using clinical specimens for validation and commercial real-time PCR as the reference method. Results The analytical sensitivity of the RAA assay was 2.23 copies per reaction, and no cross-reactions with any of the other 15 related respiratory bacterial pathogens were observed. Compared with the commercial real-time PCR assay used when testing 311 respiratory specimens, the RAA assay obtained 100% sensitivity and 100% specificity with a kappa value of 1. Conclusions These results demonstrate that the proposed RAA assay will be of benefit as a faster, sensitive, and specific alternative tool for the detection of M. pneumoniae. |
| first_indexed | 2024-12-22T19:12:26Z |
| format | Article |
| id | doaj.art-190add129595473bab5b8bfd4d7ef261 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-22T19:12:26Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-190add129595473bab5b8bfd4d7ef2612022-12-21T18:15:38ZengBMCBMC Infectious Diseases1471-23342020-01-012011710.1186/s12879-019-4750-4Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detectionGuanhua Xue0Shaoli Li1Hanqing Zhao2Chao Yan3Yanling Feng4Jinghua Cui5Tingting Jiang6Jing Yuan7Department of Bacteriology, Capital Institute of PediatricsDepartment of Bacteriology, Capital Institute of PediatricsDepartment of Bacteriology, Capital Institute of PediatricsDepartment of Bacteriology, Capital Institute of PediatricsDepartment of Bacteriology, Capital Institute of PediatricsDepartment of Bacteriology, Capital Institute of PediatricsDepartment of Obstetrics, Southern District of the Fifth Medical Center of PLA General HospitalDepartment of Bacteriology, Capital Institute of PediatricsAbstract Background Mycoplasma pneumoniae is one of the most common causative pathogens of community-acquired pneumonia (CAP), accounting for as many as 30–50% of CAP during peak years. An early and rapid diagnostic method is key for guiding clinicians in their choice of antibiotics. Methods The recombinase-aided amplification (RAA) assay is a recently developed, rapid detection method that has been used for the detection of several pathogens. The assays were performed in a one-step single tube reaction at 39° Celsius within 15–30 min. In this study, we established an RAA assay for M. pneumoniae using clinical specimens for validation and commercial real-time PCR as the reference method. Results The analytical sensitivity of the RAA assay was 2.23 copies per reaction, and no cross-reactions with any of the other 15 related respiratory bacterial pathogens were observed. Compared with the commercial real-time PCR assay used when testing 311 respiratory specimens, the RAA assay obtained 100% sensitivity and 100% specificity with a kappa value of 1. Conclusions These results demonstrate that the proposed RAA assay will be of benefit as a faster, sensitive, and specific alternative tool for the detection of M. pneumoniae.https://doi.org/10.1186/s12879-019-4750-4Mycoplasma pneumoniaeRecombinaseRecombinase-aided amplificationDetectionMolecular diagnostic technique |
| spellingShingle | Guanhua Xue Shaoli Li Hanqing Zhao Chao Yan Yanling Feng Jinghua Cui Tingting Jiang Jing Yuan Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection BMC Infectious Diseases Mycoplasma pneumoniae Recombinase Recombinase-aided amplification Detection Molecular diagnostic technique |
| title | Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection |
| title_full | Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection |
| title_fullStr | Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection |
| title_full_unstemmed | Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection |
| title_short | Use of a rapid recombinase-aided amplification assay for Mycoplasma pneumoniae detection |
| title_sort | use of a rapid recombinase aided amplification assay for mycoplasma pneumoniae detection |
| topic | Mycoplasma pneumoniae Recombinase Recombinase-aided amplification Detection Molecular diagnostic technique |
| url | https://doi.org/10.1186/s12879-019-4750-4 |
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