GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets
In pancreatic islets, the major cell-types are α, β and δ cells. The γ-aminobutyric acid (GABA) signalling system is expressed in human pancreatic islets. In single hormone transcript-expressing cells, we have previously characterized the functional properties of...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-01-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/2/600 |
| _version_ | 1811308732655599616 |
|---|---|
| author | Sergiy V. Korol Zhe Jin Bryndis Birnir |
| author_facet | Sergiy V. Korol Zhe Jin Bryndis Birnir |
| author_sort | Sergiy V. Korol |
| collection | DOAJ |
| description | In pancreatic islets, the major cell-types are α, β and δ cells. The γ-aminobutyric acid (GABA) signalling system is expressed in human pancreatic islets. In single hormone transcript-expressing cells, we have previously characterized the functional properties of islet GABA<sub>A</sub> receptors (iGABA<sub>A</sub>Rs). Here, we extended these studies to islet cells expressing mRNAs for more than one hormone and sought for correlation between iGABA<sub>A</sub>R activity level and relative mRNA expression ratio. The single-cell RT-PCR in combination with the patch-clamp current recordings was used to examine functional properties of iGABA<sub>A</sub>Rs in the multiple hormone mRNA-expressing cells. We detected cells expressing double (α/β, α/δ, β/δ cell-types) and triple (α/β/δ cell-type) hormone transcripts. The most common mixed-identity cell-type was the α/β group where the cells could be grouped into β- and α-like subgroups. The β-like cells had low <i>GCG</i>/<i>INS</i> expression ratio (<0.6) and significantly higher frequency of iGABA<sub>A</sub>R single-channel openings than the α-like cells where the <i>GCG</i>/<i>INS</i> expression ratio was high (>1.2). The hormone expression levels and iGABA<sub>A</sub>R single-channel characteristics varied in the α/β/δ cell-type. Clearly, multiple hormone transcripts can be expressed in islet cells whereas iGABA<sub>A</sub>R single-channel functional properties appear to be α or β cell specific. |
| first_indexed | 2024-04-13T09:28:36Z |
| format | Article |
| id | doaj.art-190b41e6ec104abda86319d74edcb0ac |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T09:28:36Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-190b41e6ec104abda86319d74edcb0ac2022-12-22T02:52:19ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-01-0121260010.3390/ijms21020600ijms21020600GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic IsletsSergiy V. Korol0Zhe Jin1Bryndis Birnir2Department of Medical Cell Biology, Uppsala University, BMC, Box 593, 75124 Uppsala, SwedenDepartment of Medical Cell Biology, Uppsala University, BMC, Box 593, 75124 Uppsala, SwedenDepartment of Medical Cell Biology, Uppsala University, BMC, Box 593, 75124 Uppsala, SwedenIn pancreatic islets, the major cell-types are α, β and δ cells. The γ-aminobutyric acid (GABA) signalling system is expressed in human pancreatic islets. In single hormone transcript-expressing cells, we have previously characterized the functional properties of islet GABA<sub>A</sub> receptors (iGABA<sub>A</sub>Rs). Here, we extended these studies to islet cells expressing mRNAs for more than one hormone and sought for correlation between iGABA<sub>A</sub>R activity level and relative mRNA expression ratio. The single-cell RT-PCR in combination with the patch-clamp current recordings was used to examine functional properties of iGABA<sub>A</sub>Rs in the multiple hormone mRNA-expressing cells. We detected cells expressing double (α/β, α/δ, β/δ cell-types) and triple (α/β/δ cell-type) hormone transcripts. The most common mixed-identity cell-type was the α/β group where the cells could be grouped into β- and α-like subgroups. The β-like cells had low <i>GCG</i>/<i>INS</i> expression ratio (<0.6) and significantly higher frequency of iGABA<sub>A</sub>R single-channel openings than the α-like cells where the <i>GCG</i>/<i>INS</i> expression ratio was high (>1.2). The hormone expression levels and iGABA<sub>A</sub>R single-channel characteristics varied in the α/β/δ cell-type. Clearly, multiple hormone transcripts can be expressed in islet cells whereas iGABA<sub>A</sub>R single-channel functional properties appear to be α or β cell specific.https://www.mdpi.com/1422-0067/21/2/600α-like cellβ cellgabaglucagoninsulinmixed-identity cell |
| spellingShingle | Sergiy V. Korol Zhe Jin Bryndis Birnir GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets International Journal of Molecular Sciences α-like cell β cell gaba glucagon insulin mixed-identity cell |
| title | GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets |
| title_full | GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets |
| title_fullStr | GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets |
| title_full_unstemmed | GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets |
| title_short | GABA<sub>A</sub> Receptor-Mediated Currents and Hormone mRNAs in Cells Expressing More Than One Hormone Transcript in Intact Human Pancreatic Islets |
| title_sort | gaba sub a sub receptor mediated currents and hormone mrnas in cells expressing more than one hormone transcript in intact human pancreatic islets |
| topic | α-like cell β cell gaba glucagon insulin mixed-identity cell |
| url | https://www.mdpi.com/1422-0067/21/2/600 |
| work_keys_str_mv | AT sergiyvkorol gabasubasubreceptormediatedcurrentsandhormonemrnasincellsexpressingmorethanonehormonetranscriptinintacthumanpancreaticislets AT zhejin gabasubasubreceptormediatedcurrentsandhormonemrnasincellsexpressingmorethanonehormonetranscriptinintacthumanpancreaticislets AT bryndisbirnir gabasubasubreceptormediatedcurrentsandhormonemrnasincellsexpressingmorethanonehormonetranscriptinintacthumanpancreaticislets |