Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms
IntroductionMarine epiphytic diatoms are natural diets for multiple marine invertebrates, including sea cucumbers, sea urchins, etc. They also act as bioindicators in marine waters to assess water quality of coastal ecosystems. Material and methodsWe isolated three strains of Navicula and two strain...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1292713/full |
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| author | Miao Yang Xin-Yue Xu Han-Wen Hu Wei-Dong Zhang Jing-Yi Ma Heng-Ping Lei Qing-Zhi Wang Qing-Zhi Wang Xi Xie Xi Xie Zheng Gong |
| author_facet | Miao Yang Xin-Yue Xu Han-Wen Hu Wei-Dong Zhang Jing-Yi Ma Heng-Ping Lei Qing-Zhi Wang Qing-Zhi Wang Xi Xie Xi Xie Zheng Gong |
| author_sort | Miao Yang |
| collection | DOAJ |
| description | IntroductionMarine epiphytic diatoms are natural diets for multiple marine invertebrates, including sea cucumbers, sea urchins, etc. They also act as bioindicators in marine waters to assess water quality of coastal ecosystems. Material and methodsWe isolated three strains of Navicula and two strains of Nitzschia from macroalgal epiphytes. Combined concentrations of four macroelements, five trace metals, and three vitamins in f/2 medium were optimized for these diatoms using single-factor and orthogonal tests. Results and discussionThe biomass productivities for 4-L enlarged cultures of the five isolated diatoms in their exclusively optimized f/2 media all increased by nearly 10-fold relative to f/2 medium, and that of Navicula-1 and Nitzschia-2 reached the highest, i.e., 29.52 ± 1.98 and 30.68 ± 2.84 g m-2 d-1, respectively. Moreover, the relative abundance of C20:5n3 in the exclusively optimized f/2 medium-cultured Navicula-1 notably rose by 24%, and that in Nitzschia-2 held stable at > 25%. Thus, Navicula-1 and Nitzschia-2 were screened out with outstanding growth traits and improved fatty acid compositions at high levels of combined nitrogen, phosphorus, iron, and silicon. The two diatoms have potential for becoming quality diets for marine aquaculture, and ideal candidates for effective nutrient removal. |
| first_indexed | 2024-03-08T23:28:25Z |
| format | Article |
| id | doaj.art-190baf63300d4c0eb8fe0d4e15aa3d08 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-03-08T23:28:25Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-190baf63300d4c0eb8fe0d4e15aa3d082023-12-14T16:44:33ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-12-011010.3389/fmars.2023.12927131292713Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatomsMiao Yang0Xin-Yue Xu1Han-Wen Hu2Wei-Dong Zhang3Jing-Yi Ma4Heng-Ping Lei5Qing-Zhi Wang6Qing-Zhi Wang7Xi Xie8Xi Xie9Zheng Gong10Key Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaDalian Key Laboratory of Genetic Resources for Marine Shellfish, Liaoning Ocean and Fisheries Science Research Institute, Dalian, ChinaKey Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Dalian, ChinaDalian Key Laboratory of Genetic Resources for Marine Shellfish, Liaoning Ocean and Fisheries Science Research Institute, Dalian, ChinaKey Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs, Dalian, ChinaKey Laboratory of Plant Biotechnology of Liaoning Province, School of Life Sciences, Liaoning Normal University, Dalian, ChinaIntroductionMarine epiphytic diatoms are natural diets for multiple marine invertebrates, including sea cucumbers, sea urchins, etc. They also act as bioindicators in marine waters to assess water quality of coastal ecosystems. Material and methodsWe isolated three strains of Navicula and two strains of Nitzschia from macroalgal epiphytes. Combined concentrations of four macroelements, five trace metals, and three vitamins in f/2 medium were optimized for these diatoms using single-factor and orthogonal tests. Results and discussionThe biomass productivities for 4-L enlarged cultures of the five isolated diatoms in their exclusively optimized f/2 media all increased by nearly 10-fold relative to f/2 medium, and that of Navicula-1 and Nitzschia-2 reached the highest, i.e., 29.52 ± 1.98 and 30.68 ± 2.84 g m-2 d-1, respectively. Moreover, the relative abundance of C20:5n3 in the exclusively optimized f/2 medium-cultured Navicula-1 notably rose by 24%, and that in Nitzschia-2 held stable at > 25%. Thus, Navicula-1 and Nitzschia-2 were screened out with outstanding growth traits and improved fatty acid compositions at high levels of combined nitrogen, phosphorus, iron, and silicon. The two diatoms have potential for becoming quality diets for marine aquaculture, and ideal candidates for effective nutrient removal.https://www.frontiersin.org/articles/10.3389/fmars.2023.1292713/fullNaviculaNitzschiamacroelementsbiomass productivityfatty acid profile |
| spellingShingle | Miao Yang Xin-Yue Xu Han-Wen Hu Wei-Dong Zhang Jing-Yi Ma Heng-Ping Lei Qing-Zhi Wang Qing-Zhi Wang Xi Xie Xi Xie Zheng Gong Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms Frontiers in Marine Science Navicula Nitzschia macroelements biomass productivity fatty acid profile |
| title | Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms |
| title_full | Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms |
| title_fullStr | Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms |
| title_full_unstemmed | Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms |
| title_short | Combined application of nitrogen, phosphorus, iron, and silicon improves growth and fatty acid composition in marine epiphytic diatoms |
| title_sort | combined application of nitrogen phosphorus iron and silicon improves growth and fatty acid composition in marine epiphytic diatoms |
| topic | Navicula Nitzschia macroelements biomass productivity fatty acid profile |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1292713/full |
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