Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene
Abstract Eighty percent of the estimated 600 million domestic cats in the world are free-roaming. These cats typically experience suboptimal welfare and inflict high levels of predation on wildlife. Additionally, euthanasia of healthy animals in overpopulated shelters raises ethical considerations....
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-38721-0 |
| _version_ | 1797806555417018368 |
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| author | Lindsey M. Vansandt Marie-Charlotte Meinsohn Philippe Godin Nicholas Nagykery Natalie Sicher Motohiro Kano Aki Kashiwagi Maeva Chauvin Hatice D. Saatcioglu Julie L. Barnes Amy G. Miller Amy K. Thompson Helen L. Bateman Elizabeth M. Donelan Raquel González Jackie Newsom Guangping Gao Patricia K. Donahoe Dan Wang William F. Swanson David Pépin |
| author_facet | Lindsey M. Vansandt Marie-Charlotte Meinsohn Philippe Godin Nicholas Nagykery Natalie Sicher Motohiro Kano Aki Kashiwagi Maeva Chauvin Hatice D. Saatcioglu Julie L. Barnes Amy G. Miller Amy K. Thompson Helen L. Bateman Elizabeth M. Donelan Raquel González Jackie Newsom Guangping Gao Patricia K. Donahoe Dan Wang William F. Swanson David Pépin |
| author_sort | Lindsey M. Vansandt |
| collection | DOAJ |
| description | Abstract Eighty percent of the estimated 600 million domestic cats in the world are free-roaming. These cats typically experience suboptimal welfare and inflict high levels of predation on wildlife. Additionally, euthanasia of healthy animals in overpopulated shelters raises ethical considerations. While surgical sterilization is the mainstay of pet population control, there is a need for efficient, safe, and cost-effective permanent contraception alternatives. Herein, we report evidence that a single intramuscular treatment with an adeno-associated viral vector delivering an anti-Müllerian hormone transgene produces long-term contraception in the domestic cat. Treated females are followed for over two years, during which transgene expression, anti-transgene antibodies, and reproductive hormones are monitored. Mating behavior and reproductive success are measured during two mating studies. Here we show that ectopic expression of anti-Müllerian hormone does not impair sex steroids nor estrous cycling, but prevents breeding-induced ovulation, resulting in safe and durable contraception in the female domestic cat. |
| first_indexed | 2024-03-13T06:09:02Z |
| format | Article |
| id | doaj.art-190d36565fa84c9e9cbd0f745505260e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-13T06:09:02Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-190d36565fa84c9e9cbd0f745505260e2023-06-11T11:20:00ZengNature PortfolioNature Communications2041-17232023-06-0114111210.1038/s41467-023-38721-0Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgeneLindsey M. Vansandt0Marie-Charlotte Meinsohn1Philippe Godin2Nicholas Nagykery3Natalie Sicher4Motohiro Kano5Aki Kashiwagi6Maeva Chauvin7Hatice D. Saatcioglu8Julie L. Barnes9Amy G. Miller10Amy K. Thompson11Helen L. Bateman12Elizabeth M. Donelan13Raquel González14Jackie Newsom15Guangping Gao16Patricia K. Donahoe17Dan Wang18William F. Swanson19David Pépin20Center for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenHorae Gene Therapy Center, University of Massachusetts Chan Medical SchoolPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolHorae Gene Therapy Center, University of Massachusetts Chan Medical SchoolCenter for Conservation and Research of Endangered Wildlife (CREW), Cincinnati Zoo & Botanical GardenPediatric Surgical Research Laboratories, Massachusetts General Hospital, Department of Surgery, Harvard Medical SchoolAbstract Eighty percent of the estimated 600 million domestic cats in the world are free-roaming. These cats typically experience suboptimal welfare and inflict high levels of predation on wildlife. Additionally, euthanasia of healthy animals in overpopulated shelters raises ethical considerations. While surgical sterilization is the mainstay of pet population control, there is a need for efficient, safe, and cost-effective permanent contraception alternatives. Herein, we report evidence that a single intramuscular treatment with an adeno-associated viral vector delivering an anti-Müllerian hormone transgene produces long-term contraception in the domestic cat. Treated females are followed for over two years, during which transgene expression, anti-transgene antibodies, and reproductive hormones are monitored. Mating behavior and reproductive success are measured during two mating studies. Here we show that ectopic expression of anti-Müllerian hormone does not impair sex steroids nor estrous cycling, but prevents breeding-induced ovulation, resulting in safe and durable contraception in the female domestic cat.https://doi.org/10.1038/s41467-023-38721-0 |
| spellingShingle | Lindsey M. Vansandt Marie-Charlotte Meinsohn Philippe Godin Nicholas Nagykery Natalie Sicher Motohiro Kano Aki Kashiwagi Maeva Chauvin Hatice D. Saatcioglu Julie L. Barnes Amy G. Miller Amy K. Thompson Helen L. Bateman Elizabeth M. Donelan Raquel González Jackie Newsom Guangping Gao Patricia K. Donahoe Dan Wang William F. Swanson David Pépin Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene Nature Communications |
| title | Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene |
| title_full | Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene |
| title_fullStr | Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene |
| title_full_unstemmed | Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene |
| title_short | Durable contraception in the female domestic cat using viral-vectored delivery of a feline anti-Müllerian hormone transgene |
| title_sort | durable contraception in the female domestic cat using viral vectored delivery of a feline anti mullerian hormone transgene |
| url | https://doi.org/10.1038/s41467-023-38721-0 |
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