Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil
The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological chara...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Embrapa Informação Tecnológica
2007-09-01
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| Series: | Pesquisa Agropecuária Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900016 |
| _version_ | 1818042543466610688 |
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| author | Renata Faier Calegario Sávio de Siqueira Ferreira Eduardo Chumbinho de Andrade Francisco Murilo Zerbini |
| author_facet | Renata Faier Calegario Sávio de Siqueira Ferreira Eduardo Chumbinho de Andrade Francisco Murilo Zerbini |
| author_sort | Renata Faier Calegario |
| collection | DOAJ |
| description | The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and phylogenetic analyses indicated that TGV-[Bi2] constitutes a novel begomovirus species named Tomato yellow spot virus (ToYSV), closely related to Sida mottle virus (SiMoV).<br>O objetivo deste trabalho foi a caracterização biológica e molecular de um begomovírus detectado em tomateiros em São Joaquim de Bicas, Minas Gerais, denominado TGV-[Bi2]. A caracterização biológica consistiu em teste de gama de hospedeiros, realizado por meio de inoculação via extrato foliar tamponado ou bombardeamento de partículas. O isolado TGV-[Bi2] infecta plantas das famílias Solanaceae e Amaranthaceae, inclusive espécies economicamente importantes como o pimentão, e algumas plantas daninhas como Datura stramonium e Nicotiana silvestris. A caracterização molecular consistiu na clonagem e seqüenciamento de seu genoma completo (DNA-A e DNA-B). A comparação de seqüências e análise filogenética indicaram que o TGV-[Bi2] constitui uma nova espécie de begomovírus, denominada Tomato yellow spot virus (ToYSV), filogeneticamente relacionado ao Sida mottle virus (SiMoV). |
| first_indexed | 2024-12-10T08:47:59Z |
| format | Article |
| id | doaj.art-190f4dc8385f407d91025c0a31913368 |
| institution | Directory Open Access Journal |
| issn | 0100-204X 1678-3921 |
| language | English |
| last_indexed | 2024-12-10T08:47:59Z |
| publishDate | 2007-09-01 |
| publisher | Embrapa Informação Tecnológica |
| record_format | Article |
| series | Pesquisa Agropecuária Brasileira |
| spelling | doaj.art-190f4dc8385f407d91025c0a319133682022-12-22T01:55:41ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira0100-204X1678-39212007-09-014291335134310.1590/S0100-204X2007000900016Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no BrasilRenata Faier CalegarioSávio de Siqueira FerreiraEduardo Chumbinho de AndradeFrancisco Murilo ZerbiniThe objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and phylogenetic analyses indicated that TGV-[Bi2] constitutes a novel begomovirus species named Tomato yellow spot virus (ToYSV), closely related to Sida mottle virus (SiMoV).<br>O objetivo deste trabalho foi a caracterização biológica e molecular de um begomovírus detectado em tomateiros em São Joaquim de Bicas, Minas Gerais, denominado TGV-[Bi2]. A caracterização biológica consistiu em teste de gama de hospedeiros, realizado por meio de inoculação via extrato foliar tamponado ou bombardeamento de partículas. O isolado TGV-[Bi2] infecta plantas das famílias Solanaceae e Amaranthaceae, inclusive espécies economicamente importantes como o pimentão, e algumas plantas daninhas como Datura stramonium e Nicotiana silvestris. A caracterização molecular consistiu na clonagem e seqüenciamento de seu genoma completo (DNA-A e DNA-B). A comparação de seqüências e análise filogenética indicaram que o TGV-[Bi2] constitui uma nova espécie de begomovírus, denominada Tomato yellow spot virus (ToYSV), filogeneticamente relacionado ao Sida mottle virus (SiMoV).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900016Lycopersicon esculentumBemisia tabaciToYSVbiologia moleculargeminivírusmosca-brancaLycopersicon esculentumBemisia tabaciToYSVmolecular biologygeminiviruswhitefly |
| spellingShingle | Renata Faier Calegario Sávio de Siqueira Ferreira Eduardo Chumbinho de Andrade Francisco Murilo Zerbini Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil Pesquisa Agropecuária Brasileira Lycopersicon esculentum Bemisia tabaci ToYSV biologia molecular geminivírus mosca-branca Lycopersicon esculentum Bemisia tabaci ToYSV molecular biology geminivirus whitefly |
| title | Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil |
| title_full | Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil |
| title_fullStr | Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil |
| title_full_unstemmed | Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil |
| title_short | Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil |
| title_sort | characterization of tomato yellow spot virus a novel tomato infecting begomovirus in brazil caracterizacao do tomato yellow spot virus um novo begomovirus isolado de tomateiro no brasil |
| topic | Lycopersicon esculentum Bemisia tabaci ToYSV biologia molecular geminivírus mosca-branca Lycopersicon esculentum Bemisia tabaci ToYSV molecular biology geminivirus whitefly |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900016 |
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