In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology
Summary Living systems are studied using three complementary approaches: living cells, cell‐free systems and computer‐mediated modelling. Progresses in understanding, allowing researchers to create novel chassis and industrial processes rest on a cycle that combines in vivo, in vitro and in silico s...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13937 |
| _version_ | 1817985892790304768 |
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| author | Antoine Danchin |
| author_facet | Antoine Danchin |
| author_sort | Antoine Danchin |
| collection | DOAJ |
| description | Summary Living systems are studied using three complementary approaches: living cells, cell‐free systems and computer‐mediated modelling. Progresses in understanding, allowing researchers to create novel chassis and industrial processes rest on a cycle that combines in vivo, in vitro and in silico studies. This design–build–test–learn iteration loop cycle between experiments and analyses combines together physiology, genetics, biochemistry and bioinformatics in a way that keeps going forward. Because computer‐aided approaches are not directly constrained by the material nature of the entities of interest, we illustrate here how this virtuous cycle allows researchers to explore chemistry which is foreign to that present in extant life, from whole chassis to novel metabolic cycles. Particular emphasis is placed on the importance of evolution. |
| first_indexed | 2024-04-14T00:03:41Z |
| format | Article |
| id | doaj.art-1913f61c8bdb428fa2436e03c9750acf |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-14T00:03:41Z |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-1913f61c8bdb428fa2436e03c9750acf2022-12-22T02:23:37ZengWileyMicrobial Biotechnology1751-79152022-01-01151426410.1111/1751-7915.13937In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biologyAntoine Danchin0Kodikos Labs Institut Cochin 24 rue du Faubourg Saint‐Jacques Paris 75014 FranceSummary Living systems are studied using three complementary approaches: living cells, cell‐free systems and computer‐mediated modelling. Progresses in understanding, allowing researchers to create novel chassis and industrial processes rest on a cycle that combines in vivo, in vitro and in silico studies. This design–build–test–learn iteration loop cycle between experiments and analyses combines together physiology, genetics, biochemistry and bioinformatics in a way that keeps going forward. Because computer‐aided approaches are not directly constrained by the material nature of the entities of interest, we illustrate here how this virtuous cycle allows researchers to explore chemistry which is foreign to that present in extant life, from whole chassis to novel metabolic cycles. Particular emphasis is placed on the importance of evolution.https://doi.org/10.1111/1751-7915.13937 |
| spellingShingle | Antoine Danchin In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology Microbial Biotechnology |
| title | In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology |
| title_full | In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology |
| title_fullStr | In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology |
| title_full_unstemmed | In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology |
| title_short | In vivo, in vitro and in silico: an open space for the development of microbe‐based applications of synthetic biology |
| title_sort | in vivo in vitro and in silico an open space for the development of microbe based applications of synthetic biology |
| url | https://doi.org/10.1111/1751-7915.13937 |
| work_keys_str_mv | AT antoinedanchin invivoinvitroandinsilicoanopenspaceforthedevelopmentofmicrobebasedapplicationsofsyntheticbiology |