Production of transgenic pigs over-expressing the antiviral gene Mx1
The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 g...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2014-01-01
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| Series: | Cell Regeneration |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2045976917300251 |
| _version_ | 1818316911652372480 |
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| author | Quanmei Yan Huaqiang Yang Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Nana Fan Hongsheng Ouyang Weiwang Gu Liangxue Lai |
| author_facet | Quanmei Yan Huaqiang Yang Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Nana Fan Hongsheng Ouyang Weiwang Gu Liangxue Lai |
| author_sort | Quanmei Yan |
| collection | DOAJ |
| description | The myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs with influenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profound decrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-fold reduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found that the Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These results demonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate that the Mx1 transgene can be harnessed to develop disease-resistant pigs. |
| first_indexed | 2024-12-13T09:28:57Z |
| format | Article |
| id | doaj.art-1918c9c3ef4247f2ba6c4b74bf1b45f8 |
| institution | Directory Open Access Journal |
| issn | 2045-9769 |
| language | English |
| last_indexed | 2024-12-13T09:28:57Z |
| publishDate | 2014-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Cell Regeneration |
| spelling | doaj.art-1918c9c3ef4247f2ba6c4b74bf1b45f82022-12-21T23:52:33ZengSpringerOpenCell Regeneration2045-97692014-01-013110.1186/2045-9769-3-11Production of transgenic pigs over-expressing the antiviral gene Mx1Quanmei Yan0Huaqiang Yang1Dongshan Yang2Bentian Zhao3Zhen Ouyang4Zhaoming Liu5Nana Fan6Hongsheng Ouyang7Weiwang Gu8Liangxue Lai9Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaCollege of Animal Sciences, Jilin University, Changchun, ChinaInstitute of Comparative Medicine and Center of Laboratory Animals, Southern Medical University, Guangzhou, ChinaGuangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, ChinaThe myxovirus resistance gene (Mx1) has a broad spectrum of antiviral activities. It is therefore an interesting candidate gene to improve disease resistance in farm animals. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the Mx1 gene. These transgenic pigs express approximately 15–25 times more Mx1 mRNA than non-transgenic pigs, and the protein level of Mx1 was also markedly enhanced. We challenged fibroblast cells isolated from the ear skin of transgenic and control pigs with influenza A virus and classical swine fever virus (CFSV). Indirect immunofluorescence assay (IFA) revealed a profound decrease of influenza A proliferation in Mx1 transgenic cells. Growth kinetics showed an approximately 10-fold reduction of viral copies in the transgenic cells compared to non-transgenic controls. Additionally, we found that the Mx1 transgenic cells were more resistant to CSFV infection in comparison to non-transgenic cells. These results demonstrate that the Mx1 transgene can protect against viral infection in cells of transgenic pigs and indicate that the Mx1 transgene can be harnessed to develop disease-resistant pigs.http://www.sciencedirect.com/science/article/pii/S2045976917300251Antiviral breedingInnate resistanceSomatic cell nuclear transfer |
| spellingShingle | Quanmei Yan Huaqiang Yang Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Nana Fan Hongsheng Ouyang Weiwang Gu Liangxue Lai Production of transgenic pigs over-expressing the antiviral gene Mx1 Cell Regeneration Antiviral breeding Innate resistance Somatic cell nuclear transfer |
| title | Production of transgenic pigs over-expressing the antiviral gene Mx1 |
| title_full | Production of transgenic pigs over-expressing the antiviral gene Mx1 |
| title_fullStr | Production of transgenic pigs over-expressing the antiviral gene Mx1 |
| title_full_unstemmed | Production of transgenic pigs over-expressing the antiviral gene Mx1 |
| title_short | Production of transgenic pigs over-expressing the antiviral gene Mx1 |
| title_sort | production of transgenic pigs over expressing the antiviral gene mx1 |
| topic | Antiviral breeding Innate resistance Somatic cell nuclear transfer |
| url | http://www.sciencedirect.com/science/article/pii/S2045976917300251 |
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