Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection
Ralstonia solanacearum genes that are induced during tomato infection suggested that this pathogen encounters reactive oxygen species (ROS) during bacterial wilt pathogenesis. The genomes of R. solanacearum contain multiple redundant ROS-scavenging enzymes, indirect evidence that this pathogen exper...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
2009-07-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-7-0773 |
| _version_ | 1819085160020180992 |
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| author | Zomary Flores-Cruz Caitilyn Allen |
| author_facet | Zomary Flores-Cruz Caitilyn Allen |
| author_sort | Zomary Flores-Cruz |
| collection | DOAJ |
| description | Ralstonia solanacearum genes that are induced during tomato infection suggested that this pathogen encounters reactive oxygen species (ROS) during bacterial wilt pathogenesis. The genomes of R. solanacearum contain multiple redundant ROS-scavenging enzymes, indirect evidence that this pathogen experiences intense oxidative stress during its life cycle. Over 9% of the bacterium's plant-induced genes were also upregulated by hydrogen peroxide in culture, suggesting that oxidative stress may be linked to life in the plant host. Tomato leaves infected by R. solanacearum contained hydrogen peroxide, and concentrations of this ROS increased as pathogen populations increased. Mutagenesis of a plant-induced predicted peroxidase gene, bcp, resulted in an R. solanacearum strain with reduced ability to detoxify ROS in culture. The bcp mutant caused slightly delayed bacterial wilt disease onset in tomato. Moreover, its virulence was significantly reduced on tobacco plants engineered to overproduce hydrogen peroxide, demonstrating that Bcp is necessary for detoxification of plant-derived hydrogen peroxide and providing evidence that host ROS can limit the success of this pathogen. These results reveal that R. solanacearum is exposed to ROS during pathogenesis and that it has evolved a redundant and efficient oxidative stress response to adapt to the host environment and cause disease. |
| first_indexed | 2024-12-21T20:59:56Z |
| format | Article |
| id | doaj.art-191931391859418899e388dbb1a54c2c |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T20:59:56Z |
| publishDate | 2009-07-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-191931391859418899e388dbb1a54c2c2022-12-21T18:50:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-07-0122777378210.1094/MPMI-22-7-0773Ralstonia solanacearum Encounters an Oxidative Environment During Tomato InfectionZomary Flores-CruzCaitilyn AllenRalstonia solanacearum genes that are induced during tomato infection suggested that this pathogen encounters reactive oxygen species (ROS) during bacterial wilt pathogenesis. The genomes of R. solanacearum contain multiple redundant ROS-scavenging enzymes, indirect evidence that this pathogen experiences intense oxidative stress during its life cycle. Over 9% of the bacterium's plant-induced genes were also upregulated by hydrogen peroxide in culture, suggesting that oxidative stress may be linked to life in the plant host. Tomato leaves infected by R. solanacearum contained hydrogen peroxide, and concentrations of this ROS increased as pathogen populations increased. Mutagenesis of a plant-induced predicted peroxidase gene, bcp, resulted in an R. solanacearum strain with reduced ability to detoxify ROS in culture. The bcp mutant caused slightly delayed bacterial wilt disease onset in tomato. Moreover, its virulence was significantly reduced on tobacco plants engineered to overproduce hydrogen peroxide, demonstrating that Bcp is necessary for detoxification of plant-derived hydrogen peroxide and providing evidence that host ROS can limit the success of this pathogen. These results reveal that R. solanacearum is exposed to ROS during pathogenesis and that it has evolved a redundant and efficient oxidative stress response to adapt to the host environment and cause disease.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-7-0773 |
| spellingShingle | Zomary Flores-Cruz Caitilyn Allen Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection Molecular Plant-Microbe Interactions |
| title | Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection |
| title_full | Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection |
| title_fullStr | Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection |
| title_full_unstemmed | Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection |
| title_short | Ralstonia solanacearum Encounters an Oxidative Environment During Tomato Infection |
| title_sort | ralstonia solanacearum encounters an oxidative environment during tomato infection |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-7-0773 |
| work_keys_str_mv | AT zomaryflorescruz ralstoniasolanacearumencountersanoxidativeenvironmentduringtomatoinfection AT caitilynallen ralstoniasolanacearumencountersanoxidativeenvironmentduringtomatoinfection |