Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices

Using redox-sensitive fluorescent probes to record real-time reactive oxygen species production in cells from mouse carotid body slices

Summary: Reactive oxygen species (ROS) are important signaling molecules for physiologic processes such as acute response to hypoxia. However, reliable real-time ROS measurement in cells has been a long-standing methodological challenge. Here, we present a protocol to record acute changes in ROS pro...

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Bibliographic Details
Main Authors: Lin Gao, Ignacio Arias-Mayenco, Patricia Ortega-Sáenz, José López-Barneo
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Metabolism
Microscopy
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002422

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