MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana
Abstract Background Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana. Results In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of Ma...
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-02868-z |
| _version_ | 1818410755550085120 |
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| author | Jie Gao Tongxin Dou Weidi He Ou Sheng Fangcheng Bi Guiming Deng Huijun Gao Tao Dong Chunyu Li Sheng Zhang Ganjun Yi Chunhua Hu Qiaosong Yang |
| author_facet | Jie Gao Tongxin Dou Weidi He Ou Sheng Fangcheng Bi Guiming Deng Huijun Gao Tao Dong Chunyu Li Sheng Zhang Ganjun Yi Chunhua Hu Qiaosong Yang |
| author_sort | Jie Gao |
| collection | DOAJ |
| description | Abstract Background Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana. Results In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. ‘Dajiao’; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expression level of MaICE1 was significantly decreased in MaMAPK3 RNAi transgenic plants. Over-expression of MaICE1 in Cavendish banana (Musa spp. AAA group) indicated that the cold resistance of transgenic plants was superior to that of the WT plants. The POD P7 gene was significantly up-regulated in MaICE1-overexpressing transgenic plants compared with WT plants, and the POD P7 was proved to interact with MaICE1. Conclusions Taken together, our work provided new and solid evidence that MaMAPK3-MaICE1-MaPOD P7 pathway positively improved the cold tolerance in monocotyledon banana, shedding light on molecular breeding for the cold-tolerant banana or other agricultural species. |
| first_indexed | 2024-12-14T10:20:34Z |
| format | Article |
| id | doaj.art-191aaa1ea4b143a9a49b5d856f347621 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-14T10:20:34Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-191aaa1ea4b143a9a49b5d856f3476212022-12-21T23:06:36ZengBMCBMC Plant Biology1471-22292021-02-0121111810.1186/s12870-021-02868-zMaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in bananaJie Gao0Tongxin Dou1Weidi He2Ou Sheng3Fangcheng Bi4Guiming Deng5Huijun Gao6Tao Dong7Chunyu Li8Sheng Zhang9Ganjun Yi10Chunhua Hu11Qiaosong Yang12Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Biotechnology, Cornell UniversityInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchInstitute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences; Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Tropical and Subtropical Fruit Tree ResearchAbstract Background Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana. Results In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. ‘Dajiao’; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expression level of MaICE1 was significantly decreased in MaMAPK3 RNAi transgenic plants. Over-expression of MaICE1 in Cavendish banana (Musa spp. AAA group) indicated that the cold resistance of transgenic plants was superior to that of the WT plants. The POD P7 gene was significantly up-regulated in MaICE1-overexpressing transgenic plants compared with WT plants, and the POD P7 was proved to interact with MaICE1. Conclusions Taken together, our work provided new and solid evidence that MaMAPK3-MaICE1-MaPOD P7 pathway positively improved the cold tolerance in monocotyledon banana, shedding light on molecular breeding for the cold-tolerant banana or other agricultural species.https://doi.org/10.1186/s12870-021-02868-zMaMAPK3MaICE1MaPOD P7Antioxidant capacityCold tolerance |
| spellingShingle | Jie Gao Tongxin Dou Weidi He Ou Sheng Fangcheng Bi Guiming Deng Huijun Gao Tao Dong Chunyu Li Sheng Zhang Ganjun Yi Chunhua Hu Qiaosong Yang MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana BMC Plant Biology MaMAPK3 MaICE1 MaPOD P7 Antioxidant capacity Cold tolerance |
| title | MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana |
| title_full | MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana |
| title_fullStr | MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana |
| title_full_unstemmed | MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana |
| title_short | MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana |
| title_sort | mamapk3 maice1 mapod p7 pathway a positive regulator of cold tolerance in banana |
| topic | MaMAPK3 MaICE1 MaPOD P7 Antioxidant capacity Cold tolerance |
| url | https://doi.org/10.1186/s12870-021-02868-z |
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