In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone
Oxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2¢-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2¢-deoxyguanosine (dG). PG in the presence of Cu...
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| Format: | Article |
| Language: | English |
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Universitas Indonesia
2015-12-01
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| Series: | Makara Journal of Science |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/science/article/view/5171/3481 |
| _version_ | 1811304768241401856 |
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| author | Budiawan Budiawan Dwi Retno Widiastuti |
| author_facet | Budiawan Budiawan Dwi Retno Widiastuti |
| author_sort | Budiawan Budiawan |
| collection | DOAJ |
| description | Oxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2¢-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2¢-deoxyguanosine (dG). PG in the presence of CuCl2 increased the 8-OHdG formation in calf thymus DNA by around 9.17 times as compared to the control (untreated DNA). In the presence of CuCl2 at 1.28×10-5 M, the 8-OHdG per dG ratio resulting from the reaction of dG with PG at various concentrations (20–150 ppm) ranged from 75.50 to 312.06 8-OHdG per 105 dG. The 8-OHdG formation increased when the PG concentration was increased from 20 ppm to 80 ppm, and then, it began to plateau around 80 ppm. On the other hand, BHT-quinone increased the formation of 8-OHdG in the presence of CuCl2 by 0.05 times as compared to the control (untreated DNA). LC-MS/MS analysis was used to identify the molecular structure of 8-OHdG, which had a base peak (M+. + 1) at m/z = 284 and two main fragments at m/z = 167.9 and m/z = 139.9. |
| first_indexed | 2024-04-13T08:13:17Z |
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| id | doaj.art-191acfc4a0e54292ac77a4d4733a46ff |
| institution | Directory Open Access Journal |
| issn | 2339-1995 2356-0851 |
| language | English |
| last_indexed | 2024-04-13T08:13:17Z |
| publishDate | 2015-12-01 |
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| series | Makara Journal of Science |
| spelling | doaj.art-191acfc4a0e54292ac77a4d4733a46ff2022-12-22T02:54:53ZengUniversitas IndonesiaMakara Journal of Science2339-19952356-08512015-12-0119416717610.7454/mss.v19i4.5171In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinoneBudiawan Budiawan0Dwi Retno Widiastuti1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, IndonesiaOxidative DNA damage caused by propyl gallate (PG) and 2,6-di-tert-butyl-p-benzoquinone (BHT-quinone, a metabolite of butylated hydroxytoluene (BHT)) was analyzed from the 8-hydroxy-2¢-deoxyguanosine (8-OHdG) formation in calf thymus DNA and DNA base, 2¢-deoxyguanosine (dG). PG in the presence of CuCl2 increased the 8-OHdG formation in calf thymus DNA by around 9.17 times as compared to the control (untreated DNA). In the presence of CuCl2 at 1.28×10-5 M, the 8-OHdG per dG ratio resulting from the reaction of dG with PG at various concentrations (20–150 ppm) ranged from 75.50 to 312.06 8-OHdG per 105 dG. The 8-OHdG formation increased when the PG concentration was increased from 20 ppm to 80 ppm, and then, it began to plateau around 80 ppm. On the other hand, BHT-quinone increased the formation of 8-OHdG in the presence of CuCl2 by 0.05 times as compared to the control (untreated DNA). LC-MS/MS analysis was used to identify the molecular structure of 8-OHdG, which had a base peak (M+. + 1) at m/z = 284 and two main fragments at m/z = 167.9 and m/z = 139.9.http://journal.ui.ac.id/index.php/science/article/view/5171/348126-di-tert-butil-p-benzoquinoneDNA adduct8-hydroxy-2¢-deoxyguanosinepropyl gallate |
| spellingShingle | Budiawan Budiawan Dwi Retno Widiastuti In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone Makara Journal of Science 2 6-di-tert-butil-p-benzoquinone DNA adduct 8-hydroxy-2¢-deoxyguanosine propyl gallate |
| title | In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone |
| title_full | In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone |
| title_fullStr | In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone |
| title_full_unstemmed | In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone |
| title_short | In Vitro Formation of 8-hydroxy-2-deoxyguanosine (8-OHdG) in Calf Thymus DNA upon Treatment of 2-deoxyguanosine with Propyl Gallate and 2,6-di-tert-butyl-p-benzoquinone |
| title_sort | in vitro formation of 8 hydroxy 2 deoxyguanosine 8 ohdg in calf thymus dna upon treatment of 2 deoxyguanosine with propyl gallate and 2 6 di tert butyl p benzoquinone |
| topic | 2 6-di-tert-butil-p-benzoquinone DNA adduct 8-hydroxy-2¢-deoxyguanosine propyl gallate |
| url | http://journal.ui.ac.id/index.php/science/article/view/5171/3481 |
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