Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i>
Chemical defoliation is an important part of cotton mechanical harvesting, which can effectively reduce the impurity content. Thidiazuron (TDZ) is the most used chemical defoliant on cotton. To better clarify the mechanism of TDZ promoting cotton leaf abscission, a greenhouse experiment was conducte...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/8/2738 |
| _version_ | 1797570597682675712 |
|---|---|
| author | Dingsha Jin Xiangru Wang Yanchao Xu Huiping Gui Hengheng Zhang Qiang Dong Ripon Kumar Sikder Guozheng Yang Meizhen Song |
| author_facet | Dingsha Jin Xiangru Wang Yanchao Xu Huiping Gui Hengheng Zhang Qiang Dong Ripon Kumar Sikder Guozheng Yang Meizhen Song |
| author_sort | Dingsha Jin |
| collection | DOAJ |
| description | Chemical defoliation is an important part of cotton mechanical harvesting, which can effectively reduce the impurity content. Thidiazuron (TDZ) is the most used chemical defoliant on cotton. To better clarify the mechanism of TDZ promoting cotton leaf abscission, a greenhouse experiment was conducted on two cotton cultivars (CRI 12 and CRI 49) by using 100 mg L<sup>−1</sup> TDZ at the eight-true-leaf stage. Results showed that TDZ significantly promoted the formation of leaf abscission zone and leaf abscission. Although the antioxidant enzyme activities were improved, the reactive oxygen species and malondialdehyde (MDA) contents of TDZ increased significantly compared with CK (water). The photosynthesis system was destroyed as net photosynthesis (Pn), transpiration rate (Tr), and stomatal conductance (Gs) decreased dramatically by TDZ. Furthermore, comparative RNA-seq analysis of the leaves showed that all of the photosynthetic related genes were downregulated and the oxidation-reduction process participated in leaf shedding caused by TDZ. Consequently, a hypothesis involving possible cross-talk between ROS metabolism and photosynthesis jointly regulating cotton leaf abscission is proposed. Our findings not only provide important insights into leaf shedding-associated changes induced by TDZ in cotton, but also highlight the possibility that the ROS and photosynthesis may play a critical role in the organ shedding process in other crops. |
| first_indexed | 2024-03-10T20:26:49Z |
| format | Article |
| id | doaj.art-191b28b6ed0746298fbc8067ec9bfdc3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:26:49Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-191b28b6ed0746298fbc8067ec9bfdc32023-11-19T21:41:19ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01218273810.3390/ijms21082738Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i>Dingsha Jin0Xiangru Wang1Yanchao Xu2Huiping Gui3Hengheng Zhang4Qiang Dong5Ripon Kumar Sikder6Guozheng Yang7Meizhen Song8State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaMOA Key Laboratory of Crop Eco-physiology and Farming system in the Middle Reaches of Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaChemical defoliation is an important part of cotton mechanical harvesting, which can effectively reduce the impurity content. Thidiazuron (TDZ) is the most used chemical defoliant on cotton. To better clarify the mechanism of TDZ promoting cotton leaf abscission, a greenhouse experiment was conducted on two cotton cultivars (CRI 12 and CRI 49) by using 100 mg L<sup>−1</sup> TDZ at the eight-true-leaf stage. Results showed that TDZ significantly promoted the formation of leaf abscission zone and leaf abscission. Although the antioxidant enzyme activities were improved, the reactive oxygen species and malondialdehyde (MDA) contents of TDZ increased significantly compared with CK (water). The photosynthesis system was destroyed as net photosynthesis (Pn), transpiration rate (Tr), and stomatal conductance (Gs) decreased dramatically by TDZ. Furthermore, comparative RNA-seq analysis of the leaves showed that all of the photosynthetic related genes were downregulated and the oxidation-reduction process participated in leaf shedding caused by TDZ. Consequently, a hypothesis involving possible cross-talk between ROS metabolism and photosynthesis jointly regulating cotton leaf abscission is proposed. Our findings not only provide important insights into leaf shedding-associated changes induced by TDZ in cotton, but also highlight the possibility that the ROS and photosynthesis may play a critical role in the organ shedding process in other crops.https://www.mdpi.com/1422-0067/21/8/2738cottonleaf abscissionROSphotosynthesiscarbohydrateRNA-seq |
| spellingShingle | Dingsha Jin Xiangru Wang Yanchao Xu Huiping Gui Hengheng Zhang Qiang Dong Ripon Kumar Sikder Guozheng Yang Meizhen Song Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i> International Journal of Molecular Sciences cotton leaf abscission ROS photosynthesis carbohydrate RNA-seq |
| title | Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i> |
| title_full | Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i> |
| title_fullStr | Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i> |
| title_full_unstemmed | Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i> |
| title_short | Chemical Defoliant Promotes Leaf Abscission by Altering ROS Metabolism and Photosynthetic Efficiency in <i>Gossypium hirsutum</i> |
| title_sort | chemical defoliant promotes leaf abscission by altering ros metabolism and photosynthetic efficiency in i gossypium hirsutum i |
| topic | cotton leaf abscission ROS photosynthesis carbohydrate RNA-seq |
| url | https://www.mdpi.com/1422-0067/21/8/2738 |
| work_keys_str_mv | AT dingshajin chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT xiangruwang chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT yanchaoxu chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT huipinggui chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT henghengzhang chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT qiangdong chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT riponkumarsikder chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT guozhengyang chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi AT meizhensong chemicaldefoliantpromotesleafabscissionbyalteringrosmetabolismandphotosyntheticefficiencyinigossypiumhirsutumi |