Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy
Summary: The procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free micr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723006974 |
| _version_ | 1797582919721549824 |
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| author | Laura García-Mendívil María Pérez-Zabalza Sam Duwé Laura Ordovás Esther Pueyo |
| author_facet | Laura García-Mendívil María Pérez-Zabalza Sam Duwé Laura Ordovás Esther Pueyo |
| author_sort | Laura García-Mendívil |
| collection | DOAJ |
| description | Summary: The procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues. We describe the steps for sample collection, tissue processing, image acquisition, and quantification of collagen and lipofuscin. This protocol avoids discrepancies between observers and can be adapted to other tissues and species.For complete details on the use and execution of this protocol, please refer to García-Mendívil et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-10T23:29:25Z |
| format | Article |
| id | doaj.art-191cf88c5f604bbc85d3b4ea02a649cc |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-10T23:29:25Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-191cf88c5f604bbc85d3b4ea02a649cc2023-11-19T04:35:30ZengElsevierSTAR Protocols2666-16672023-12-0144102730Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopyLaura García-Mendívil0María Pérez-Zabalza1Sam Duwé2Laura Ordovás3Esther Pueyo4Biomedical Signal Interpretation and Computational Simulation Group (BSICoS), Aragón Institute of Engineering Research, University of Zaragoza, 50018 Zaragoza, Spain; BSICoS, Instituto de Investigación Sanitaria Aragón (IISA), 50018 Zaragoza, Spain; Corresponding authorBiomedical Signal Interpretation and Computational Simulation Group (BSICoS), Aragón Institute of Engineering Research, University of Zaragoza, 50018 Zaragoza, Spain; BSICoS, Instituto de Investigación Sanitaria Aragón (IISA), 50018 Zaragoza, Spain; Defense University Centre (CUD), 50090 Zaragoza, Spain; Corresponding authorAdvanced Optical Microscopy Centre, Biomedical Research Institute, Hasselt University, 3500 Diepenbeek, BelgiumBiomedical Signal Interpretation and Computational Simulation Group (BSICoS), Aragón Institute of Engineering Research, University of Zaragoza, 50018 Zaragoza, Spain; BSICoS, Instituto de Investigación Sanitaria Aragón (IISA), 50018 Zaragoza, Spain; Fundación Agencia Aragonesa para la Investigación y el Desarrollo (ARAID), 50018 Zaragoza, Spain; Corresponding authorBiomedical Signal Interpretation and Computational Simulation Group (BSICoS), Aragón Institute of Engineering Research, University of Zaragoza, 50018 Zaragoza, Spain; BSICoS, Instituto de Investigación Sanitaria Aragón (IISA), 50018 Zaragoza, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 50018 Zaragoza, SpainSummary: The procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues. We describe the steps for sample collection, tissue processing, image acquisition, and quantification of collagen and lipofuscin. This protocol avoids discrepancies between observers and can be adapted to other tissues and species.For complete details on the use and execution of this protocol, please refer to García-Mendívil et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723006974MicroscopyBiotechnology and bioengineeringComputer sciences |
| spellingShingle | Laura García-Mendívil María Pérez-Zabalza Sam Duwé Laura Ordovás Esther Pueyo Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy STAR Protocols Microscopy Biotechnology and bioengineering Computer sciences |
| title | Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy |
| title_full | Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy |
| title_fullStr | Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy |
| title_full_unstemmed | Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy |
| title_short | Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy |
| title_sort | automatic quantification of myocardial remodeling features in human ventricular tissue from label free microscopy |
| topic | Microscopy Biotechnology and bioengineering Computer sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723006974 |
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