Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan
Background/Purpose: The purpose of this study is to investigate the predominant clones of carbapenem-resistant Acinetobacter baumannii (CRAB) in our hospital in Taiwan by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) technique. Methods: We collected 108 non-duplicate...
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Elsevier
2017-10-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118217300683 |
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| author | Yi-Fan Hu Charles Jia-Yin Hou Chien-Feng Kuo Nai-Yu Wang Alice Ying-Jung Wu Ching-Hsiang Leung Chang-Pan Liu Hung-I. Yeh |
| author_facet | Yi-Fan Hu Charles Jia-Yin Hou Chien-Feng Kuo Nai-Yu Wang Alice Ying-Jung Wu Ching-Hsiang Leung Chang-Pan Liu Hung-I. Yeh |
| author_sort | Yi-Fan Hu |
| collection | DOAJ |
| description | Background/Purpose: The purpose of this study is to investigate the predominant clones of carbapenem-resistant Acinetobacter baumannii (CRAB) in our hospital in Taiwan by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) technique.
Methods: We collected 108 non-duplicate A. baumannii clinical blood isolates from January 2012 to December 2013 in MacKay Memorial Hospital. PFGE and MLST were used for typing the A. baumannii isolates and for investigation of the predominant clones. Bacteria isolates were screened by polymerase chain reaction for the presence of the carbapenemase-encoding genes.
Results: All 108 isolates were classified as 33 pulsotypes by PFGE. The predominant clones were pulsotype 10 (12.04%) in 2012 and pulsotype 8 (16.67%) in 2013, respectively. The 31 predominant pulsotype isolates were typed by MLST, and ST787 (54.84%) and ST455 (45.16%) were identified. All isolates carried blaOXA-51-like genes, and blaOXA-23-like genes was founded in 101 isolates (93.52%). Other identified resistance genes included blaOXA-24-like and blaOXA-IMP.
Conclusion: To the best of our knowledge, this study is the first to describe the microbiological characteristics of CRAB ST787, which carried high genetic resistance to carbapenem, but remained susceptible to colistin. CRAB ST787 was the predominant clone in our hospital in the study period. |
| first_indexed | 2024-12-18T23:15:19Z |
| format | Article |
| id | doaj.art-19204e34405e415db0fa839306640032 |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-18T23:15:19Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-19204e34405e415db0fa8393066400322022-12-21T20:48:14ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822017-10-0150564064510.1016/j.jmii.2016.08.025Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern TaiwanYi-Fan Hu0Charles Jia-Yin Hou1Chien-Feng Kuo2Nai-Yu Wang3Alice Ying-Jung Wu4Ching-Hsiang Leung5Chang-Pan Liu6Hung-I. Yeh7Division of Infectious Diseases, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, TaiwanDivision of Cardiology, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, Taipei, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, Taipei, TaiwanDivision of Infectious Diseases, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, TaiwanDivision of Cardiology, Department of Internal Medicine, MacKay Memorial Hospital, Taipei, TaiwanBackground/Purpose: The purpose of this study is to investigate the predominant clones of carbapenem-resistant Acinetobacter baumannii (CRAB) in our hospital in Taiwan by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) technique. Methods: We collected 108 non-duplicate A. baumannii clinical blood isolates from January 2012 to December 2013 in MacKay Memorial Hospital. PFGE and MLST were used for typing the A. baumannii isolates and for investigation of the predominant clones. Bacteria isolates were screened by polymerase chain reaction for the presence of the carbapenemase-encoding genes. Results: All 108 isolates were classified as 33 pulsotypes by PFGE. The predominant clones were pulsotype 10 (12.04%) in 2012 and pulsotype 8 (16.67%) in 2013, respectively. The 31 predominant pulsotype isolates were typed by MLST, and ST787 (54.84%) and ST455 (45.16%) were identified. All isolates carried blaOXA-51-like genes, and blaOXA-23-like genes was founded in 101 isolates (93.52%). Other identified resistance genes included blaOXA-24-like and blaOXA-IMP. Conclusion: To the best of our knowledge, this study is the first to describe the microbiological characteristics of CRAB ST787, which carried high genetic resistance to carbapenem, but remained susceptible to colistin. CRAB ST787 was the predominant clone in our hospital in the study period.http://www.sciencedirect.com/science/article/pii/S1684118217300683Acinetobacter baumanniiCarbapenemase genePFGEPulsotypeMLSTSequence type |
| spellingShingle | Yi-Fan Hu Charles Jia-Yin Hou Chien-Feng Kuo Nai-Yu Wang Alice Ying-Jung Wu Ching-Hsiang Leung Chang-Pan Liu Hung-I. Yeh Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan Journal of Microbiology, Immunology and Infection Acinetobacter baumannii Carbapenemase gene PFGE Pulsotype MLST Sequence type |
| title | Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan |
| title_full | Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan |
| title_fullStr | Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan |
| title_full_unstemmed | Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan |
| title_short | Emergence of carbapenem-resistant Acinetobacter baumannii ST787 in clinical isolates from blood in a tertiary teaching hospital in Northern Taiwan |
| title_sort | emergence of carbapenem resistant acinetobacter baumannii st787 in clinical isolates from blood in a tertiary teaching hospital in northern taiwan |
| topic | Acinetobacter baumannii Carbapenemase gene PFGE Pulsotype MLST Sequence type |
| url | http://www.sciencedirect.com/science/article/pii/S1684118217300683 |
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