Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation
We aimed to investigate the effects of maternal protein restriction during mid-gestation on the skeletal muscle composition of the offspring. In the restriction treatment (RES, n = 9), cows were fed a basal diet, while in the control (CON, n = 9) group cows received the same RES diet plus the protei...
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MDPI AG
2021-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/3/860 |
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| author | Thais Correia Costa Min Du Karolina Batista Nascimento Matheus Castilho Galvão Javier Andrés Moreno Meneses Erica Beatriz Schultz Mateus Pies Gionbelli Marcio de Souza Duarte |
| author_facet | Thais Correia Costa Min Du Karolina Batista Nascimento Matheus Castilho Galvão Javier Andrés Moreno Meneses Erica Beatriz Schultz Mateus Pies Gionbelli Marcio de Souza Duarte |
| author_sort | Thais Correia Costa |
| collection | DOAJ |
| description | We aimed to investigate the effects of maternal protein restriction during mid-gestation on the skeletal muscle composition of the offspring. In the restriction treatment (RES, n = 9), cows were fed a basal diet, while in the control (CON, n = 9) group cows received the same RES diet plus the protein supplement during mid-gestation (100–200d). Samples of <i>Longissimus dorsi</i> muscle were collected from the offspring at 30d and 450d postnatal. Muscle fiber number was found to be decreased as a result of maternal protein restriction and persisted throughout the offspring’s life (<i>p</i> < 0.01). The collagen content was enhanced (<i>p</i> < 0.05) due to maternal protein restriction at 30d. <i>MHC2X</i> mRNA expression tended to be higher (<i>p</i> = 0.08) in RES 30d offspring, however, no difference (<i>p</i> > 0.05) was found among treatments at 450d. Taken together, our results suggest that maternal protein restriction during mid-gestation has major and persistent effects by reducing muscle fiber formation and may slightly increase collagen accumulation in the skeletal muscle of the offspring. Although maternal protein restriction may alter the muscle fiber metabolism by favoring the establishment of a predominant glycolytic metabolism, the postnatal environment may be a determinant factor that establishes the different proportion of muscle fiber types. |
| first_indexed | 2024-03-10T13:08:24Z |
| format | Article |
| id | doaj.art-1920d68296df45e5b31145603fd4c699 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:08:24Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1920d68296df45e5b31145603fd4c6992023-11-21T10:57:18ZengMDPI AGAnimals2076-26152021-03-0111386010.3390/ani11030860Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-GestationThais Correia Costa0Min Du1Karolina Batista Nascimento2Matheus Castilho Galvão3Javier Andrés Moreno Meneses4Erica Beatriz Schultz5Mateus Pies Gionbelli6Marcio de Souza Duarte7Department of Animal Science, Universidade Federal de Viçosa, Viçosa MG 3657-000, BrazilDepartment of Animal Sciences, Washington State University, Pullman, WA 99163, USADepartment of Animal Science, Universidade Federal de Lavras, Lavras MG 37200-900, BrazilDepartment of Animal Science, Universidade Federal de Lavras, Lavras MG 37200-900, BrazilDepartment of Animal Science, Universidade Federal de Lavras, Lavras MG 37200-900, BrazilDepartment of Animal Science, Universidade Federal Rural do Rio de Janeiro, Seropédica RJ 23897-000, BrazilDepartment of Animal Science, Universidade Federal de Lavras, Lavras MG 37200-900, BrazilDepartment of Animal Science, Universidade Federal de Viçosa, Viçosa MG 3657-000, BrazilWe aimed to investigate the effects of maternal protein restriction during mid-gestation on the skeletal muscle composition of the offspring. In the restriction treatment (RES, n = 9), cows were fed a basal diet, while in the control (CON, n = 9) group cows received the same RES diet plus the protein supplement during mid-gestation (100–200d). Samples of <i>Longissimus dorsi</i> muscle were collected from the offspring at 30d and 450d postnatal. Muscle fiber number was found to be decreased as a result of maternal protein restriction and persisted throughout the offspring’s life (<i>p</i> < 0.01). The collagen content was enhanced (<i>p</i> < 0.05) due to maternal protein restriction at 30d. <i>MHC2X</i> mRNA expression tended to be higher (<i>p</i> = 0.08) in RES 30d offspring, however, no difference (<i>p</i> > 0.05) was found among treatments at 450d. Taken together, our results suggest that maternal protein restriction during mid-gestation has major and persistent effects by reducing muscle fiber formation and may slightly increase collagen accumulation in the skeletal muscle of the offspring. Although maternal protein restriction may alter the muscle fiber metabolism by favoring the establishment of a predominant glycolytic metabolism, the postnatal environment may be a determinant factor that establishes the different proportion of muscle fiber types.https://www.mdpi.com/2076-2615/11/3/860beef cattledietary protein restrictionmaternal effectsmid-gestationskeletal muscle development |
| spellingShingle | Thais Correia Costa Min Du Karolina Batista Nascimento Matheus Castilho Galvão Javier Andrés Moreno Meneses Erica Beatriz Schultz Mateus Pies Gionbelli Marcio de Souza Duarte Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation Animals beef cattle dietary protein restriction maternal effects mid-gestation skeletal muscle development |
| title | Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation |
| title_full | Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation |
| title_fullStr | Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation |
| title_full_unstemmed | Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation |
| title_short | Skeletal Muscle Development in Postnatal Beef Cattle Resulting from Maternal Protein Restriction during Mid-Gestation |
| title_sort | skeletal muscle development in postnatal beef cattle resulting from maternal protein restriction during mid gestation |
| topic | beef cattle dietary protein restriction maternal effects mid-gestation skeletal muscle development |
| url | https://www.mdpi.com/2076-2615/11/3/860 |
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