Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities
Background: Hepatocellular carcinoma (HCC) is the most typical form of liver cancer. Apart from modern therapies, various natural chemical constituents have been tested for the treatment of HCC. However, the usage of the latter has declined due to their low bioavailability and stability. Objectives:...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364723003713 |
| _version_ | 1797653936854794240 |
|---|---|
| author | M. Lavanya Rajapandiyan Krishnamoorthy Mohammad A. Alshuniaber Salim Manoharadas Chella Perumal Palanisamy Vishnu Priya Veeraraghavan Selvaraj Jayaraman Ponnulakshmi Rajagopal Ramakrishnan Padmini |
| author_facet | M. Lavanya Rajapandiyan Krishnamoorthy Mohammad A. Alshuniaber Salim Manoharadas Chella Perumal Palanisamy Vishnu Priya Veeraraghavan Selvaraj Jayaraman Ponnulakshmi Rajagopal Ramakrishnan Padmini |
| author_sort | M. Lavanya |
| collection | DOAJ |
| description | Background: Hepatocellular carcinoma (HCC) is the most typical form of liver cancer. Apart from modern therapies, various natural chemical constituents have been tested for the treatment of HCC. However, the usage of the latter has declined due to their low bioavailability and stability. Objectives: Considering the above drawback’s, in this study, we describe a gelatin-syringic acid/ zinc oxide (ZnO) nanocomposite for liver cancer treatment, which showed antioxidant, anti-inflammatory and anticancer activities. Methods: The hydrothermal method was used to synthesize ZnO nanoparticle and it was encapsulated with gelatin-syringic acid by coacervation technique. The nanocomposites were characterized by UV spec, FTIR, SEM, XRD, EDX, and DLS. The drug release profile of nanocomposite was studied by dialysis bag method, which exhibits a sustained drug release. Results: The antioxidant ability of nano-composite was studied by performing an ABTS and DPPH assay and the IC50 was recorded as 76.5 and 47.63 µg/mL, respectively. The anti-inflammatory potential was studied by assessing the ability of nanocomposite on denaturation of protein and the results exhibited a dose-dependent inhibition. The acute toxicity of nanocomposite tested on zebrafish liver and heart showed that it is non-toxic. Moreover, the nanocomposite inhibits the Hep G2 cell viability with an increasing concentration and it increases oxidative stress caused by the mitochondrial damage, which leads to cell death. Conclusion: Based on the findings of the present study, gelatin-syringic acid/ZnO nanocomposite has been shown to possess antioxidant, and anti-inflammatory properties, and thus can be used against HCC. |
| first_indexed | 2024-03-11T16:52:05Z |
| format | Article |
| id | doaj.art-192256298b43467ba72baa89645b5848 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-03-11T16:52:05Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-192256298b43467ba72baa89645b58482023-10-21T04:22:17ZengElsevierJournal of King Saud University: Science1018-36472023-11-01358102909Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activitiesM. Lavanya0Rajapandiyan Krishnamoorthy1Mohammad A. Alshuniaber2Salim Manoharadas3Chella Perumal Palanisamy4Vishnu Priya Veeraraghavan5Selvaraj Jayaraman6Ponnulakshmi Rajagopal7Ramakrishnan Padmini8Department of Biochemistry, Vels Institute Of Science, Technology, and Advanced Studies, Vels University, Chennai 600117, IndiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Kingdom of Saudi ArabiaDepartment of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Kingdom of Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. BOX 2454, Riyadh, Saudi ArabiaState Key Laboratory of Biobased Material and Green Papermaking, College of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Science, Jinan 250353, ChinaCentre of Molecular Medicine and Diagnostics (COMManD), Department of Bio-chemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai 600077, IndiaCentre of Molecular Medicine and Diagnostics (COMManD), Department of Bio-chemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical & Technical Sciences, Saveetha University, Chennai 600077, IndiaDepartment of Central Research Laboratory, Meenakshi Ammal Dental College and Hospital, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai 600 095, India; Corresponding authors.Department of Biochemistry, Vels Institute Of Science, Technology, and Advanced Studies, Vels University, Chennai 600117, India; Corresponding authors.Background: Hepatocellular carcinoma (HCC) is the most typical form of liver cancer. Apart from modern therapies, various natural chemical constituents have been tested for the treatment of HCC. However, the usage of the latter has declined due to their low bioavailability and stability. Objectives: Considering the above drawback’s, in this study, we describe a gelatin-syringic acid/ zinc oxide (ZnO) nanocomposite for liver cancer treatment, which showed antioxidant, anti-inflammatory and anticancer activities. Methods: The hydrothermal method was used to synthesize ZnO nanoparticle and it was encapsulated with gelatin-syringic acid by coacervation technique. The nanocomposites were characterized by UV spec, FTIR, SEM, XRD, EDX, and DLS. The drug release profile of nanocomposite was studied by dialysis bag method, which exhibits a sustained drug release. Results: The antioxidant ability of nano-composite was studied by performing an ABTS and DPPH assay and the IC50 was recorded as 76.5 and 47.63 µg/mL, respectively. The anti-inflammatory potential was studied by assessing the ability of nanocomposite on denaturation of protein and the results exhibited a dose-dependent inhibition. The acute toxicity of nanocomposite tested on zebrafish liver and heart showed that it is non-toxic. Moreover, the nanocomposite inhibits the Hep G2 cell viability with an increasing concentration and it increases oxidative stress caused by the mitochondrial damage, which leads to cell death. Conclusion: Based on the findings of the present study, gelatin-syringic acid/ZnO nanocomposite has been shown to possess antioxidant, and anti-inflammatory properties, and thus can be used against HCC.http://www.sciencedirect.com/science/article/pii/S1018364723003713Zinc oxide nanoparticleSyringic acidGelatinHep G2 cell lineAntioxidantAnti-inflammatory |
| spellingShingle | M. Lavanya Rajapandiyan Krishnamoorthy Mohammad A. Alshuniaber Salim Manoharadas Chella Perumal Palanisamy Vishnu Priya Veeraraghavan Selvaraj Jayaraman Ponnulakshmi Rajagopal Ramakrishnan Padmini Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities Journal of King Saud University: Science Zinc oxide nanoparticle Syringic acid Gelatin Hep G2 cell line Antioxidant Anti-inflammatory |
| title | Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities |
| title_full | Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities |
| title_fullStr | Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities |
| title_full_unstemmed | Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities |
| title_short | Formulation, characterization and evaluation of gelatin-syringic acid/zinc oxide nanocomposite for its effective anticancer, antioxidant and anti-inflammatory activities |
| title_sort | formulation characterization and evaluation of gelatin syringic acid zinc oxide nanocomposite for its effective anticancer antioxidant and anti inflammatory activities |
| topic | Zinc oxide nanoparticle Syringic acid Gelatin Hep G2 cell line Antioxidant Anti-inflammatory |
| url | http://www.sciencedirect.com/science/article/pii/S1018364723003713 |
| work_keys_str_mv | AT mlavanya formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT rajapandiyankrishnamoorthy formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT mohammadaalshuniaber formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT salimmanoharadas formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT chellaperumalpalanisamy formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT vishnupriyaveeraraghavan formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT selvarajjayaraman formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT ponnulakshmirajagopal formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities AT ramakrishnanpadmini formulationcharacterizationandevaluationofgelatinsyringicacidzincoxidenanocompositeforitseffectiveanticancerantioxidantandantiinflammatoryactivities |