Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i>
Stress-associated proteins (SAPs), a class of A20/AN1 zinc finger proteins, play vital roles in plant stress response. However, investigation of SAPs in maize has been very limited. Herein, to better trace the evolutionary history of SAPs in maize and plants, 415 SAPs were identified in 33 plant spe...
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2022-11-01
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| author | Anqi Su Qianqian Qin Chao Liu Jiajun Zhang Bingxin Yu Yifeng Cheng Sijia Wang Jiawen Tang Weina Si |
| author_facet | Anqi Su Qianqian Qin Chao Liu Jiajun Zhang Bingxin Yu Yifeng Cheng Sijia Wang Jiawen Tang Weina Si |
| author_sort | Anqi Su |
| collection | DOAJ |
| description | Stress-associated proteins (SAPs), a class of A20/AN1 zinc finger proteins, play vital roles in plant stress response. However, investigation of SAPs in maize has been very limited. Herein, to better trace the evolutionary history of SAPs in maize and plants, 415 SAPs were identified in 33 plant species and four species of other kingdoms. Moreover, gene duplication mode exploration showed whole genome duplication contributed largely to SAP gene expansion in angiosperms. Phylogeny reconstruction was performed with all identified SAPs by the maximum likelihood (ML) method and the SAPs were divided into five clades. SAPs within the same clades showed conserved domain composition. Focusing on maize, nine <i>ZmSAPs</i> were identified. Further promoter cis-elements and stress-induced expression pattern analysis of <i>ZmSAPs</i> indicated that <i>ZmSAP8</i> was a promising candidate in response to drought stress, which was the only AN1-AN1-C2H2-C2H2 type SAP in maize and belonged to clade I. Additionally, ZmSAP8 was located in the nucleus and had no transactivation activity in yeast. Overexpressing <i>ZmSAP8</i> enhanced the tolerance to drought stress in <i>Arabidopsis thaliana</i>, with higher seed germination and longer root length. Our results should benefit the further functional characterization of <i>ZmSAPs</i>. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:16:23Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1922752f6ce24615a9a2410bb6ea53ba2023-11-24T08:38:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221410910.3390/ijms232214109Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i>Anqi Su0Qianqian Qin1Chao Liu2Jiajun Zhang3Bingxin Yu4Yifeng Cheng5Sijia Wang6Jiawen Tang7Weina Si8National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaNational Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei 230036, ChinaStress-associated proteins (SAPs), a class of A20/AN1 zinc finger proteins, play vital roles in plant stress response. However, investigation of SAPs in maize has been very limited. Herein, to better trace the evolutionary history of SAPs in maize and plants, 415 SAPs were identified in 33 plant species and four species of other kingdoms. Moreover, gene duplication mode exploration showed whole genome duplication contributed largely to SAP gene expansion in angiosperms. Phylogeny reconstruction was performed with all identified SAPs by the maximum likelihood (ML) method and the SAPs were divided into five clades. SAPs within the same clades showed conserved domain composition. Focusing on maize, nine <i>ZmSAPs</i> were identified. Further promoter cis-elements and stress-induced expression pattern analysis of <i>ZmSAPs</i> indicated that <i>ZmSAP8</i> was a promising candidate in response to drought stress, which was the only AN1-AN1-C2H2-C2H2 type SAP in maize and belonged to clade I. Additionally, ZmSAP8 was located in the nucleus and had no transactivation activity in yeast. Overexpressing <i>ZmSAP8</i> enhanced the tolerance to drought stress in <i>Arabidopsis thaliana</i>, with higher seed germination and longer root length. Our results should benefit the further functional characterization of <i>ZmSAPs</i>.https://www.mdpi.com/1422-0067/23/22/14109stress-associated proteinA20/AN1 domainmaizeabiotic stressgene family |
| spellingShingle | Anqi Su Qianqian Qin Chao Liu Jiajun Zhang Bingxin Yu Yifeng Cheng Sijia Wang Jiawen Tang Weina Si Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i> International Journal of Molecular Sciences stress-associated protein A20/AN1 domain maize abiotic stress gene family |
| title | Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i> |
| title_full | Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i> |
| title_fullStr | Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i> |
| title_short | Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of <i>ZmSAP8</i> in Transgenic <i>Arabidopsis</i> |
| title_sort | identification and analysis of stress associated proteins saps protein family and drought tolerance of i zmsap8 i in transgenic i arabidopsis i |
| topic | stress-associated protein A20/AN1 domain maize abiotic stress gene family |
| url | https://www.mdpi.com/1422-0067/23/22/14109 |
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