Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China
Abstract Background Human adenoviruses (HAdV) have been known to cause a range of diseases, including respiratory tract infections (RTIs). However, there is limited information available regarding the genotype diversity and epidemiology of HAdV associated with RTIs in Nanning. Methods Between June 2...
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| Format: | Article |
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BMC
2023-08-01
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| Series: | Virology Journal |
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| Online Access: | https://doi.org/10.1186/s12985-023-02093-0 |
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| author | Jianqiu Qin Tengyue Yan Liujiang Yin Cheng Yang Liang Wang Hong Qiu Yanling Hu Bin Xu |
| author_facet | Jianqiu Qin Tengyue Yan Liujiang Yin Cheng Yang Liang Wang Hong Qiu Yanling Hu Bin Xu |
| author_sort | Jianqiu Qin |
| collection | DOAJ |
| description | Abstract Background Human adenoviruses (HAdV) have been known to cause a range of diseases, including respiratory tract infections (RTIs). However, there is limited information available regarding the genotype diversity and epidemiology of HAdV associated with RTIs in Nanning. Methods Between June 2019 and December 2021, throat swab, nasal swab, or nasopharyngeal swab samples were obtained from individuals hospitalized with respiratory tract infections (RTIs). Statistical software was used to analyze the epidemiological data. The highly conserved 132-bp gene region of the HAdV hexon was targeted for the detection of HAdV using a qPCR assay. An 875-bp hexon gene fragment was subjected to phylogenetic analysis. Results Significant variations were observed in the age and gender distribution of HAdV-positive patients (P = 0.004 and P = 0.025, respectively). The age distribution of HAdV-positive patients showed that 67.89% of those who tested positive were the age group of 0–6 years. Furthermore, the prevalence of HAdV detection was highest during spring and autumn, with a peak in February. Additionally, genotyping of the 36 HAdV-positive samples with 875-bp fragments identified the presence of circulating HAdV species B, C, and E in Nanning between 2019 and 2021. Conclusions This study identified an association between HAdV prevalence and age as well as season. Among hospitalized patients with RTIs in Nanning, HAdV-B, HAdV-C, and HAdV-E were found to be co-circulating. The most commonly detected genotypes were HAdV-C1, HAdV-C6, and HAdV-E4. |
| first_indexed | 2024-03-12T17:09:57Z |
| format | Article |
| id | doaj.art-19236fec608b42309e880efe4c399e0a |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-03-12T17:09:57Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-19236fec608b42309e880efe4c399e0a2023-08-06T11:06:42ZengBMCVirology Journal1743-422X2023-08-012011810.1186/s12985-023-02093-0Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, ChinaJianqiu Qin0Tengyue Yan1Liujiang Yin2Cheng Yang3Liang Wang4Hong Qiu5Yanling Hu6Bin Xu7Nanning Center for Disease Control and PreventionCollaborative Innovation Centre of Regenerative Medicine and Medical Bioresource Development and Application Co-constructed by the Province and Ministry, Guangxi Medical UniversityNanning Center for Disease Control and PreventionNanning Center for Disease Control and PreventionNanning Center for Disease Control and PreventionInstitute of Life Sciences, Guangxi Medical UniversityCollaborative Innovation Centre of Regenerative Medicine and Medical Bioresource Development and Application Co-constructed by the Province and Ministry, Guangxi Medical UniversityNanning Center for Disease Control and PreventionAbstract Background Human adenoviruses (HAdV) have been known to cause a range of diseases, including respiratory tract infections (RTIs). However, there is limited information available regarding the genotype diversity and epidemiology of HAdV associated with RTIs in Nanning. Methods Between June 2019 and December 2021, throat swab, nasal swab, or nasopharyngeal swab samples were obtained from individuals hospitalized with respiratory tract infections (RTIs). Statistical software was used to analyze the epidemiological data. The highly conserved 132-bp gene region of the HAdV hexon was targeted for the detection of HAdV using a qPCR assay. An 875-bp hexon gene fragment was subjected to phylogenetic analysis. Results Significant variations were observed in the age and gender distribution of HAdV-positive patients (P = 0.004 and P = 0.025, respectively). The age distribution of HAdV-positive patients showed that 67.89% of those who tested positive were the age group of 0–6 years. Furthermore, the prevalence of HAdV detection was highest during spring and autumn, with a peak in February. Additionally, genotyping of the 36 HAdV-positive samples with 875-bp fragments identified the presence of circulating HAdV species B, C, and E in Nanning between 2019 and 2021. Conclusions This study identified an association between HAdV prevalence and age as well as season. Among hospitalized patients with RTIs in Nanning, HAdV-B, HAdV-C, and HAdV-E were found to be co-circulating. The most commonly detected genotypes were HAdV-C1, HAdV-C6, and HAdV-E4.https://doi.org/10.1186/s12985-023-02093-0Human adenovirusesRespiratory infectionsNanningPrevalenceHexon gene |
| spellingShingle | Jianqiu Qin Tengyue Yan Liujiang Yin Cheng Yang Liang Wang Hong Qiu Yanling Hu Bin Xu Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China Virology Journal Human adenoviruses Respiratory infections Nanning Prevalence Hexon gene |
| title | Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China |
| title_full | Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China |
| title_fullStr | Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China |
| title_full_unstemmed | Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China |
| title_short | Detection of human adenoviruses in influenza-negative patients with respiratory tract infections in Nanning, China |
| title_sort | detection of human adenoviruses in influenza negative patients with respiratory tract infections in nanning china |
| topic | Human adenoviruses Respiratory infections Nanning Prevalence Hexon gene |
| url | https://doi.org/10.1186/s12985-023-02093-0 |
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