N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool
MicroRNAs (miRNAs or miRs) play crucial roles in biological and pathological processes. Some miRNAs also appear as promising biomarkers and therapeutic tools. However, the epitranscriptomic regulation of miRNAs is not yet fully elucidated in all of their fields of application. We report that adenosi...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Molecular Therapy: Nucleic Acids |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253120302390 |
| _version_ | 1818448685908885504 |
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| author | Joséphine Briand Aurélien A. Sérandour Arulraj Nadaradjane Gwenola Bougras-Cartron Dominique Heymann Benjamin Ory François M. Vallette Pierre-François Cartron |
| author_facet | Joséphine Briand Aurélien A. Sérandour Arulraj Nadaradjane Gwenola Bougras-Cartron Dominique Heymann Benjamin Ory François M. Vallette Pierre-François Cartron |
| author_sort | Joséphine Briand |
| collection | DOAJ |
| description | MicroRNAs (miRNAs or miRs) play crucial roles in biological and pathological processes. Some miRNAs also appear as promising biomarkers and therapeutic tools. However, the epitranscriptomic regulation of miRNAs is not yet fully elucidated in all of their fields of application. We report that adenosine methylation of miR-200b-3p inhibits its repressive function toward its mRNA targets such as XIAP by blocking the formation of the miRNA/3′ UTRmRNA duplex. Our data indicate that the adenosine methylation of miR-200b-3p is associated with the survival of glioblastoma patients. Collectively, our data support the idea that the adenosine methylation of miR-200b-3p can be used as a prodrug having a selective cytotoxicity against cancer cells (while being harmless to peripheral blood mononuclear cells [PBMCs], astrocytes, neurons, and hepatocytes). |
| first_indexed | 2024-12-14T20:23:27Z |
| format | Article |
| id | doaj.art-1923c4a8e922482c889c4a8688391f88 |
| institution | Directory Open Access Journal |
| issn | 2162-2531 |
| language | English |
| last_indexed | 2024-12-14T20:23:27Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Therapy: Nucleic Acids |
| spelling | doaj.art-1923c4a8e922482c889c4a8688391f882022-12-21T22:48:41ZengElsevierMolecular Therapy: Nucleic Acids2162-25312020-12-01227283N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic ToolJoséphine Briand0Aurélien A. Sérandour1Arulraj Nadaradjane2Gwenola Bougras-Cartron3Dominique Heymann4Benjamin Ory5François M. Vallette6Pierre-François Cartron7CRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, France; Cancéropole Grand-Ouest, Réseau Niches et Epigénétique des Tumeurs (NET), Nantes, France; EpiSAVMEN Network (Région Pays de la Loire), Nantes, FranceCRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, France; Cancéropole Grand-Ouest, Réseau Niches et Epigénétique des Tumeurs (NET), Nantes, France; EpiSAVMEN Network (Région Pays de la Loire), Nantes, France; Ecole Centrale Nantes, Nantes, FranceCRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, France; Cancéropole Grand-Ouest, Réseau Niches et Epigénétique des Tumeurs (NET), Nantes, France; EpiSAVMEN Network (Région Pays de la Loire), Nantes, France; LabEX IGO, Université de Nantes, Nantes, FranceCRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, France; Cancéropole Grand-Ouest, Réseau Niches et Epigénétique des Tumeurs (NET), Nantes, France; EpiSAVMEN Network (Région Pays de la Loire), Nantes, FranceCRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, FranceCancéropole Grand-Ouest, Réseau Niches et Epigénétique des Tumeurs (NET), Nantes, France; EpiSAVMEN Network (Région Pays de la Loire), Nantes, France; INSERM, U1238, Université de Nantes, Nantes, FranceCRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, France; LabEX IGO, Université de Nantes, Nantes, FranceCRCINA, INSERM, Université de Nantes, Nantes, France; Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Saint Herblain, France; Cancéropole Grand-Ouest, Réseau Niches et Epigénétique des Tumeurs (NET), Nantes, France; EpiSAVMEN Network (Région Pays de la Loire), Nantes, France; LabEX IGO, Université de Nantes, Nantes, France; Corresponding author: Pierre-François Cartron, Equipe Apoptose et Progression Tumorale, LaBCT, Institut de Cancérologie de l’Ouest, Boulevard du Professeur Jacques Monod, 44805 Saint Herblain, France.MicroRNAs (miRNAs or miRs) play crucial roles in biological and pathological processes. Some miRNAs also appear as promising biomarkers and therapeutic tools. However, the epitranscriptomic regulation of miRNAs is not yet fully elucidated in all of their fields of application. We report that adenosine methylation of miR-200b-3p inhibits its repressive function toward its mRNA targets such as XIAP by blocking the formation of the miRNA/3′ UTRmRNA duplex. Our data indicate that the adenosine methylation of miR-200b-3p is associated with the survival of glioblastoma patients. Collectively, our data support the idea that the adenosine methylation of miR-200b-3p can be used as a prodrug having a selective cytotoxicity against cancer cells (while being harmless to peripheral blood mononuclear cells [PBMCs], astrocytes, neurons, and hepatocytes).http://www.sciencedirect.com/science/article/pii/S2162253120302390miRNA methylationglioblastomaadenosine methylationprodrugbiomarker |
| spellingShingle | Joséphine Briand Aurélien A. Sérandour Arulraj Nadaradjane Gwenola Bougras-Cartron Dominique Heymann Benjamin Ory François M. Vallette Pierre-François Cartron N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool Molecular Therapy: Nucleic Acids miRNA methylation glioblastoma adenosine methylation prodrug biomarker |
| title | N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool |
| title_full | N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool |
| title_fullStr | N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool |
| title_full_unstemmed | N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool |
| title_short | N6-Adenosine Methylation of miRNA-200b-3p Influences Its Functionality and Is a Theranostic Tool |
| title_sort | n6 adenosine methylation of mirna 200b 3p influences its functionality and is a theranostic tool |
| topic | miRNA methylation glioblastoma adenosine methylation prodrug biomarker |
| url | http://www.sciencedirect.com/science/article/pii/S2162253120302390 |
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