A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies
Abstract Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA g...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Oxford University Press
2023-03-01
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| Series: | ISME Communications |
| Online Access: | https://doi.org/10.1038/s43705-023-00220-0 |
| _version_ | 1797230369972420608 |
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| author | Stephanie M. Rosales Lindsay K. Huebner James S. Evans Amy Apprill Andrew C. Baker Cynthia C. Becker Anthony J. Bellantuono Marilyn E. Brandt Abigail S. Clark Javier del Campo Caroline E. Dennison Katherine R. Eaton Naomi E. Huntley Christina A. Kellogg Mónica Medina Julie L. Meyer Erinn M. Muller Mauricio Rodriguez-Lanetty Jennifer L. Salerno William B. Schill Erin N. Shilling Julia Marie Stewart Joshua D. Voss |
| author_facet | Stephanie M. Rosales Lindsay K. Huebner James S. Evans Amy Apprill Andrew C. Baker Cynthia C. Becker Anthony J. Bellantuono Marilyn E. Brandt Abigail S. Clark Javier del Campo Caroline E. Dennison Katherine R. Eaton Naomi E. Huntley Christina A. Kellogg Mónica Medina Julie L. Meyer Erinn M. Muller Mauricio Rodriguez-Lanetty Jennifer L. Salerno William B. Schill Erin N. Shilling Julia Marie Stewart Joshua D. Voss |
| author_sort | Stephanie M. Rosales |
| collection | DOAJ |
| description | Abstract Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA gene datasets generated by 16 field and laboratory SCTLD studies to find consistent bacteria associated with SCTLD across disease zones (vulnerable, endemic, and epidemic), coral species, coral compartments (mucus, tissue, and skeleton), and colony health states (apparently healthy colony tissue (AH), and unaffected (DU) and lesion (DL) tissue from diseased colonies). We also evaluated bacteria in seawater and sediment, which may be sources of SCTLD transmission. Although AH colonies in endemic and epidemic zones harbor bacteria associated with SCTLD lesions, and aquaria and field samples had distinct microbial compositions, there were still clear differences in the microbial composition among AH, DU, and DL in the combined dataset. Alpha-diversity between AH and DL was not different; however, DU showed increased alpha-diversity compared to AH, indicating that, prior to lesion formation, corals may undergo a disturbance to the microbiome. This disturbance may be driven by Flavobacteriales, which were especially enriched in DU. In DL, Rhodobacterales and Peptostreptococcales–Tissierellales were prominent in structuring microbial interactions. We also predict an enrichment of an alpha-toxin in DL samples which is typically found in Clostridia. We provide a consensus of SCTLD-associated bacteria prior to and during lesion formation and identify how these taxa vary across studies, coral species, coral compartments, seawater, and sediment. |
| first_indexed | 2024-04-24T15:27:24Z |
| format | Article |
| id | doaj.art-19245052d610410dbd29142dd37eeac7 |
| institution | Directory Open Access Journal |
| issn | 2730-6151 |
| language | English |
| last_indexed | 2024-04-24T15:27:24Z |
| publishDate | 2023-03-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | ISME Communications |
| spelling | doaj.art-19245052d610410dbd29142dd37eeac72024-04-02T05:43:36ZengOxford University PressISME Communications2730-61512023-03-013111410.1038/s43705-023-00220-0A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased coloniesStephanie M. Rosales0Lindsay K. Huebner1James S. Evans2Amy Apprill3Andrew C. Baker4Cynthia C. Becker5Anthony J. Bellantuono6Marilyn E. Brandt7Abigail S. Clark8Javier del Campo9Caroline E. Dennison10Katherine R. Eaton11Naomi E. Huntley12Christina A. Kellogg13Mónica Medina14Julie L. Meyer15Erinn M. Muller16Mauricio Rodriguez-Lanetty17Jennifer L. Salerno18William B. Schill19Erin N. Shilling20Julia Marie Stewart21Joshua D. Voss22The University of Miami, Cooperative Institute for Marine and Atmospheric StudiesFlorida Fish and Wildlife Conservation Commission, Fish and Wildlife Research InstituteU.S. Geological Survey, St. Petersburg Coastal and Marine Science CenterWoods Hole Oceanographic Institution, Marine Chemistry and GeochemistryThe University of Miami, Rosenstiel School of Marine, Atmospheric, and Earth Science, Department of Marine Biology and EcologyWoods Hole Oceanographic Institution, Marine Chemistry and GeochemistryFlorida International University, Department of Biological SciencesThe University of the Virgin Islands, Center for Marine and Environmental StudiesThe College of the Florida Keys, Marine Science and TechnologyInstitut de Biologia Evolutiva (CSIC - Universitat Pompeu Fabra)-BarcelonaThe University of Miami, Rosenstiel School of Marine, Atmospheric, and Earth Science, Department of Marine Biology and EcologyThe University of Miami, Cooperative Institute for Marine and Atmospheric StudiesThe Pennsylvania State University, Biology DepartmentU.S. Geological Survey, St. Petersburg Coastal and Marine Science CenterThe Pennsylvania State University, Biology DepartmentUniversity of Florida, Soil, Water, and Ecosystem Sciences DepartmentMote Marine Laboratory, Coral Health and Disease ProgramFlorida International University, Department of Biological SciencesGeorge Mason University, Potomac Environmental Research and Education Center, Department of Environmental Science and PolicyU.S. Geological Survey, Eastern Ecological Science CenterHarbor Branch Oceanographic Institute, Florida Atlantic UniversityThe Pennsylvania State University, Biology DepartmentHarbor Branch Oceanographic Institute, Florida Atlantic UniversityAbstract Stony coral tissue loss disease (SCTLD) has been causing significant whole colony mortality on reefs in Florida and the Caribbean. The cause of SCTLD remains unknown, with the limited concurrence of SCTLD-associated bacteria among studies. We conducted a meta-analysis of 16S ribosomal RNA gene datasets generated by 16 field and laboratory SCTLD studies to find consistent bacteria associated with SCTLD across disease zones (vulnerable, endemic, and epidemic), coral species, coral compartments (mucus, tissue, and skeleton), and colony health states (apparently healthy colony tissue (AH), and unaffected (DU) and lesion (DL) tissue from diseased colonies). We also evaluated bacteria in seawater and sediment, which may be sources of SCTLD transmission. Although AH colonies in endemic and epidemic zones harbor bacteria associated with SCTLD lesions, and aquaria and field samples had distinct microbial compositions, there were still clear differences in the microbial composition among AH, DU, and DL in the combined dataset. Alpha-diversity between AH and DL was not different; however, DU showed increased alpha-diversity compared to AH, indicating that, prior to lesion formation, corals may undergo a disturbance to the microbiome. This disturbance may be driven by Flavobacteriales, which were especially enriched in DU. In DL, Rhodobacterales and Peptostreptococcales–Tissierellales were prominent in structuring microbial interactions. We also predict an enrichment of an alpha-toxin in DL samples which is typically found in Clostridia. We provide a consensus of SCTLD-associated bacteria prior to and during lesion formation and identify how these taxa vary across studies, coral species, coral compartments, seawater, and sediment.https://doi.org/10.1038/s43705-023-00220-0 |
| spellingShingle | Stephanie M. Rosales Lindsay K. Huebner James S. Evans Amy Apprill Andrew C. Baker Cynthia C. Becker Anthony J. Bellantuono Marilyn E. Brandt Abigail S. Clark Javier del Campo Caroline E. Dennison Katherine R. Eaton Naomi E. Huntley Christina A. Kellogg Mónica Medina Julie L. Meyer Erinn M. Muller Mauricio Rodriguez-Lanetty Jennifer L. Salerno William B. Schill Erin N. Shilling Julia Marie Stewart Joshua D. Voss A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies ISME Communications |
| title | A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies |
| title_full | A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies |
| title_fullStr | A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies |
| title_full_unstemmed | A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies |
| title_short | A meta-analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies |
| title_sort | meta analysis of the stony coral tissue loss disease microbiome finds key bacteria in unaffected and lesion tissue in diseased colonies |
| url | https://doi.org/10.1038/s43705-023-00220-0 |
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