Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method
Introduction: Vancomycin Resistant Enterococci (VRE) can be found all over the world. Thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. Aim: To measure the turanose fermentation in Enterococcus faecalis clinical isolates...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2017-03-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/9568/19017_CE[Ra1]_F(GH)_PF1(ESPI_RK)_PFA(AK)_PF2(P_NESY).pdf |
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| author | Javad Raeisi Mahnaz Saifi Mohammad Reza Pourshafie Mehri Habibi Hamid Reza Mohajerani Neda Akbari Mohammad Reza Asadi Karam |
| author_facet | Javad Raeisi Mahnaz Saifi Mohammad Reza Pourshafie Mehri Habibi Hamid Reza Mohajerani Neda Akbari Mohammad Reza Asadi Karam |
| author_sort | Javad Raeisi |
| collection | DOAJ |
| description | Introduction: Vancomycin Resistant Enterococci (VRE) can be
found all over the world. Thus, rapid detection of the isolates
could be of high importance in the treatment or prevention of
the associated disease.
Aim: To measure the turanose fermentation in Enterococcus
faecalis clinical isolates for rapid differentiation of VRE and
Vancomycin-Susceptible E. faecalis (VSE) isolates.
Materials and Methods: Forty E. faecalis samples were isolated
from 200 clinical samples in Tehran Medical Center, Iran, from
October 2012 to December 2012. These isolates were detected
according to the standard microbial and biochemical tests.
Detection of VRE isolates was originally performed by disk
diffusion using 1 μg vancomycin disk, followed by Polymerase
Chain Reaction (PCR) amplification of the vanA gene. Finally,
the turanose consumption in 1%, 0.7% and 0.5% dilutions was
detected by a phenotypic method.
Results: Among the 40 E. faecalis isolates, 20 vancomycinsusceptible and 20 vancomycin-resistant E. faecalis were
isolated according to the disk diffusion and PCR of the vanA
gene. There was a considerable difference between VRE and
VSE isolates in 0.7% dilution of turanose. However, there was
no significant difference between VRE and VSE in 1% and 0.5%
dilutions of turanose.
Conclusion: Since detection of VRE isolates is of high
importance, especially in nosocomial infections, phenotypic
methods may be highly useful for this purpose. In conclusion,
our data indicate that VRE isolated from clinical samples could
be distinguished from VSE isolates by turanose fermentation at
dilution 0.7%. |
| first_indexed | 2024-12-19T21:41:41Z |
| format | Article |
| id | doaj.art-192903670938496b9e234e47166eea95 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-19T21:41:41Z |
| publishDate | 2017-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-192903670938496b9e234e47166eea952022-12-21T20:04:38ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-03-01113DC14DC1710.7860/JCDR/2017/19017.9568Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation MethodJavad Raeisi0Mahnaz Saifi1Mohammad Reza Pourshafie2Mehri Habibi3Hamid Reza Mohajerani4Neda Akbari5Mohammad Reza Asadi Karam6Postgraduate Student, Department of Microbiology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.Associate Professor, Department of Mycobacteriology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.Professor, Department of Microbiology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.Assistant Professor, Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.Assistant Professor, Department of Microbiology, Islamic Azad University, Arak branch, Arak, Iran.Assistant Professor, Department of Microbiology, Islamic Azad University, Arak branch, Arak, Iran.Assistant Professor, Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.Introduction: Vancomycin Resistant Enterococci (VRE) can be found all over the world. Thus, rapid detection of the isolates could be of high importance in the treatment or prevention of the associated disease. Aim: To measure the turanose fermentation in Enterococcus faecalis clinical isolates for rapid differentiation of VRE and Vancomycin-Susceptible E. faecalis (VSE) isolates. Materials and Methods: Forty E. faecalis samples were isolated from 200 clinical samples in Tehran Medical Center, Iran, from October 2012 to December 2012. These isolates were detected according to the standard microbial and biochemical tests. Detection of VRE isolates was originally performed by disk diffusion using 1 μg vancomycin disk, followed by Polymerase Chain Reaction (PCR) amplification of the vanA gene. Finally, the turanose consumption in 1%, 0.7% and 0.5% dilutions was detected by a phenotypic method. Results: Among the 40 E. faecalis isolates, 20 vancomycinsusceptible and 20 vancomycin-resistant E. faecalis were isolated according to the disk diffusion and PCR of the vanA gene. There was a considerable difference between VRE and VSE isolates in 0.7% dilution of turanose. However, there was no significant difference between VRE and VSE in 1% and 0.5% dilutions of turanose. Conclusion: Since detection of VRE isolates is of high importance, especially in nosocomial infections, phenotypic methods may be highly useful for this purpose. In conclusion, our data indicate that VRE isolated from clinical samples could be distinguished from VSE isolates by turanose fermentation at dilution 0.7%.https://jcdr.net/articles/PDF/9568/19017_CE[Ra1]_F(GH)_PF1(ESPI_RK)_PFA(AK)_PF2(P_NESY).pdfantibiotic resistancephenotypic methodsturanose consumption |
| spellingShingle | Javad Raeisi Mahnaz Saifi Mohammad Reza Pourshafie Mehri Habibi Hamid Reza Mohajerani Neda Akbari Mohammad Reza Asadi Karam Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method Journal of Clinical and Diagnostic Research antibiotic resistance phenotypic methods turanose consumption |
| title | Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method |
| title_full | Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method |
| title_fullStr | Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method |
| title_full_unstemmed | Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method |
| title_short | Rapid Identification of Vancomycin Resistant Enterococcus Faecalis Clinical Isolates using a Sugar Fermentation Method |
| title_sort | rapid identification of vancomycin resistant enterococcus faecalis clinical isolates using a sugar fermentation method |
| topic | antibiotic resistance phenotypic methods turanose consumption |
| url | https://jcdr.net/articles/PDF/9568/19017_CE[Ra1]_F(GH)_PF1(ESPI_RK)_PFA(AK)_PF2(P_NESY).pdf |
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