The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018
Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD. This study was conducted to determine the most common allergens by the skin pr...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2023-11-01
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| Series: | Medical Laboratory Journal |
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| Online Access: | http://mlj.goums.ac.ir/article-1-1382-en.pdf |
| _version_ | 1797220380245491712 |
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| author | Mohammad Sadegh Naghizadeh Mohsen Naseri Gholamreza Anani Sarab Afshin Derakhshani Mohammad Fereidouni |
| author_facet | Mohammad Sadegh Naghizadeh Mohsen Naseri Gholamreza Anani Sarab Afshin Derakhshani Mohammad Fereidouni |
| author_sort | Mohammad Sadegh Naghizadeh |
| collection | DOAJ |
| description | Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD. This study was conducted to determine the most common allergens by the skin prick test (SPT) and immunoblotting among AD patients referring to an allergy clinic in Birjand City, Iran.
Methods: The presence of AD was confirmed by an expert allergist. Serum levels of total and specific immunoglobulin E (sIgE) against 30 food and inhalant allergens were evaluated by a commercial immunoblotting kit (AlleisaScreen).
Results: The skin prick test was performed by a battery of 17 allergens. In total, 34 AD patients (mean age, 28.76 ± 17.36 years; range, 1-60 years; F/M ratio: 0.88) were enrolled in this study. The sensitization rates to at least 1 fungus, pollen, food, or indoor allergen by the immunoblotting method were 32.35%, 61.76%, 52.94%, and 47.05%, respectively. The most prevalent allergens were ragweed (52.94), Olive tree (41.16), Eucalyptus (35.29), date palm (35.29), and grass mix (32.28).
Conclusion: The study found that 85.29% of the studied population were sensitized to at least 1 allergen. Pollens and date palms were the most common allergens among AD patients, but the pattern of sensitization in SPT and immunoblotting was not exactly similar. Detection of allergens to which patients are sensitized and avoidance can help in the management of the disease and its symptoms. |
| first_indexed | 2024-04-24T12:48:37Z |
| format | Article |
| id | doaj.art-1929517472f54bb8beb04d0b4541bcbd |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-04-24T12:48:37Z |
| publishDate | 2023-11-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-1929517472f54bb8beb04d0b4541bcbd2024-04-06T09:52:07ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492023-11-011761315The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018Mohammad Sadegh Naghizadeh0Mohsen Naseri1Gholamreza Anani Sarab2Afshin Derakhshani3Mohammad Fereidouni4 . Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran . Student Research Committee, Birjand University of Medical Sciences, Birjand, Iran Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran Background: Atopic dermatitis (AD) is a common allergic disorder. Detection of responsible pathogenic allergens in AD patients by reliable methods has a fundamental role in the prevention, management, and treatment of AD. This study was conducted to determine the most common allergens by the skin prick test (SPT) and immunoblotting among AD patients referring to an allergy clinic in Birjand City, Iran. Methods: The presence of AD was confirmed by an expert allergist. Serum levels of total and specific immunoglobulin E (sIgE) against 30 food and inhalant allergens were evaluated by a commercial immunoblotting kit (AlleisaScreen). Results: The skin prick test was performed by a battery of 17 allergens. In total, 34 AD patients (mean age, 28.76 ± 17.36 years; range, 1-60 years; F/M ratio: 0.88) were enrolled in this study. The sensitization rates to at least 1 fungus, pollen, food, or indoor allergen by the immunoblotting method were 32.35%, 61.76%, 52.94%, and 47.05%, respectively. The most prevalent allergens were ragweed (52.94), Olive tree (41.16), Eucalyptus (35.29), date palm (35.29), and grass mix (32.28). Conclusion: The study found that 85.29% of the studied population were sensitized to at least 1 allergen. Pollens and date palms were the most common allergens among AD patients, but the pattern of sensitization in SPT and immunoblotting was not exactly similar. Detection of allergens to which patients are sensitized and avoidance can help in the management of the disease and its symptoms.http://mlj.goums.ac.ir/article-1-1382-en.pdfallergensatopic dermatitisreceptors igeimmunoblottingskin prick test (skin tests) |
| spellingShingle | Mohammad Sadegh Naghizadeh Mohsen Naseri Gholamreza Anani Sarab Afshin Derakhshani Mohammad Fereidouni The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 Medical Laboratory Journal allergens atopic dermatitis receptors ige immunoblotting skin prick test (skin tests) |
| title | The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 |
| title_full | The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 |
| title_fullStr | The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 |
| title_full_unstemmed | The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 |
| title_short | The pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 |
| title_sort | pattern of allergic sensitization by the skin prick test and immunoblotting method among patients with atopic dermatitis in 2018 |
| topic | allergens atopic dermatitis receptors ige immunoblotting skin prick test (skin tests) |
| url | http://mlj.goums.ac.ir/article-1-1382-en.pdf |
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