Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR
Programmed mini-pumps play a significant role in various fields, such as chemistry, biology, and medicine, to transport a measured volume of liquid, especially in the current detection of COVID-19 with PCR. In view of the cost of the current automatic pipetting pump being higher, which is difficult...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/5/772 |
| _version_ | 1797497752437915648 |
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| author | Chengxiong Lin Yaocheng Wang Zhengyu Huang Yu Guo Wenming Wu |
| author_facet | Chengxiong Lin Yaocheng Wang Zhengyu Huang Yu Guo Wenming Wu |
| author_sort | Chengxiong Lin |
| collection | DOAJ |
| description | Programmed mini-pumps play a significant role in various fields, such as chemistry, biology, and medicine, to transport a measured volume of liquid, especially in the current detection of COVID-19 with PCR. In view of the cost of the current automatic pipetting pump being higher, which is difficult to use in a regular lab, this paper designed and assembled a three-dimensional programmed mini-pump with the common parts and components, such as PLC controller, motor, microinjector, etc. With the weighting calibration before and after pipetting operation, the error of the pipette in 10 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mi mathvariant="sans-serif">μ</mi><mi mathvariant="normal">L</mi></mrow></mrow></semantics></math></inline-formula> (0.2%), 2 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mi mathvariant="sans-serif">μ</mi><mi mathvariant="normal">L</mi></mrow></mrow></semantics></math></inline-formula> (1.8%), and 1 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mi mathvariant="sans-serif">μ</mi><mi mathvariant="normal">L</mi></mrow></mrow></semantics></math></inline-formula> (5.6%) can be obtained. Besides, the contrast test between three-dimensional programmed mini-pump and manual pipette was conducted with the ORF1ab and pGEM-3Zf (+) genes in qPCR. The results proved that the custom-made three-dimensional programmed mini-pump has a stronger reproducibility compared with manual pipette (ORF1ab: 24.06 ± 0.33 vs. 23.50 ± 0.58, <i>p</i> = 0.1014; pGEM-3Zf (+): 11.83.06 ± 0.24 vs. 11.50 ± 0.34, <i>p</i> = 0.8779). These results can lay the foundation for the functional, fast, and low-cost programmed mini-pump in PCR or other applications for trace measurements. |
| first_indexed | 2024-03-10T03:23:45Z |
| format | Article |
| id | doaj.art-1929f7c68e1f4f22976dab6c731803db |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T03:23:45Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
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| series | Micromachines |
| spelling | doaj.art-1929f7c68e1f4f22976dab6c731803db2023-11-23T12:12:57ZengMDPI AGMicromachines2072-666X2022-05-0113577210.3390/mi13050772Low Cost Three-Dimensional Programmed Mini-Pump Used in PCRChengxiong Lin0Yaocheng Wang1Zhengyu Huang2Yu Guo3Wenming Wu4National Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Polymer Material Products, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaNational Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Polymer Material Products, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaNational Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Polymer Material Products, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaNational Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Polymer Material Products, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaNational Engineering Research Center for Healthcare Devices, Guangdong Provincial Key Laboratory of Medical Electronic Instruments and Polymer Material Products, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510316, ChinaProgrammed mini-pumps play a significant role in various fields, such as chemistry, biology, and medicine, to transport a measured volume of liquid, especially in the current detection of COVID-19 with PCR. In view of the cost of the current automatic pipetting pump being higher, which is difficult to use in a regular lab, this paper designed and assembled a three-dimensional programmed mini-pump with the common parts and components, such as PLC controller, motor, microinjector, etc. With the weighting calibration before and after pipetting operation, the error of the pipette in 10 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mi mathvariant="sans-serif">μ</mi><mi mathvariant="normal">L</mi></mrow></mrow></semantics></math></inline-formula> (0.2%), 2 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mi mathvariant="sans-serif">μ</mi><mi mathvariant="normal">L</mi></mrow></mrow></semantics></math></inline-formula> (1.8%), and 1 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mrow><mi mathvariant="sans-serif">μ</mi><mi mathvariant="normal">L</mi></mrow></mrow></semantics></math></inline-formula> (5.6%) can be obtained. Besides, the contrast test between three-dimensional programmed mini-pump and manual pipette was conducted with the ORF1ab and pGEM-3Zf (+) genes in qPCR. The results proved that the custom-made three-dimensional programmed mini-pump has a stronger reproducibility compared with manual pipette (ORF1ab: 24.06 ± 0.33 vs. 23.50 ± 0.58, <i>p</i> = 0.1014; pGEM-3Zf (+): 11.83.06 ± 0.24 vs. 11.50 ± 0.34, <i>p</i> = 0.8779). These results can lay the foundation for the functional, fast, and low-cost programmed mini-pump in PCR or other applications for trace measurements.https://www.mdpi.com/2072-666X/13/5/772mini-pumpPCRORF1abpipetting accuracycorrection method |
| spellingShingle | Chengxiong Lin Yaocheng Wang Zhengyu Huang Yu Guo Wenming Wu Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR Micromachines mini-pump PCR ORF1ab pipetting accuracy correction method |
| title | Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR |
| title_full | Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR |
| title_fullStr | Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR |
| title_full_unstemmed | Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR |
| title_short | Low Cost Three-Dimensional Programmed Mini-Pump Used in PCR |
| title_sort | low cost three dimensional programmed mini pump used in pcr |
| topic | mini-pump PCR ORF1ab pipetting accuracy correction method |
| url | https://www.mdpi.com/2072-666X/13/5/772 |
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