PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize
Chloroplasts play an essential role in plant growth and development. Any factors affecting chloroplast development will lead to abnormal plant growth. Here, we characterized a new maize mutant, <i>albino seedling mutant 81647</i> (<i>as-81647</i>), which exhibits an entirely...
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/20/11162 |
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| author | Yan Zhao Wei Xu Yongzhong Zhang Shilei Sun Lijing Wang Shiyi Zhong Xiangyu Zhao Baoshen Liu |
| author_facet | Yan Zhao Wei Xu Yongzhong Zhang Shilei Sun Lijing Wang Shiyi Zhong Xiangyu Zhao Baoshen Liu |
| author_sort | Yan Zhao |
| collection | DOAJ |
| description | Chloroplasts play an essential role in plant growth and development. Any factors affecting chloroplast development will lead to abnormal plant growth. Here, we characterized a new maize mutant, <i>albino seedling mutant 81647</i> (<i>as-81647</i>), which exhibits an entirely albino phenotype in leaves and eventually died before the three-leaf stage. Transmission electron microscopy (TEM) demonstrated that the chloroplast thylakoid membrane was impaired and the granum lamellae significantly decreased in <i>as-81647</i>. Map-based cloning and transgenic analysis confirmed that <i>PPR647</i> encodes a new chloroplast protein consisting of 11 pentratricopeptide repeat domains. Quantitative real-time PCR (qRT-PCR) assays and transcriptome analysis (RNA-seq) showed that the <i>PPR647</i> mutation significantly disrupted the expression of PEP-dependent plastid genes. In addition, RNA splicing and RNA editing of multiple chloroplast genes showed severe defects in <i>as-81647</i>. These results indicated that PPR647 is crucial for RNA editing, RNA splicing of chloroplast genes, and plays an essential role in chloroplast development. |
| first_indexed | 2024-03-10T06:29:31Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:29:31Z |
| publishDate | 2021-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-192a0269d0b24807873d5cfd007fe8792023-11-22T18:34:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122201116210.3390/ijms222011162PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in MaizeYan Zhao0Wei Xu1Yongzhong Zhang2Shilei Sun3Lijing Wang4Shiyi Zhong5Xiangyu Zhao6Baoshen Liu7State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaChloroplasts play an essential role in plant growth and development. Any factors affecting chloroplast development will lead to abnormal plant growth. Here, we characterized a new maize mutant, <i>albino seedling mutant 81647</i> (<i>as-81647</i>), which exhibits an entirely albino phenotype in leaves and eventually died before the three-leaf stage. Transmission electron microscopy (TEM) demonstrated that the chloroplast thylakoid membrane was impaired and the granum lamellae significantly decreased in <i>as-81647</i>. Map-based cloning and transgenic analysis confirmed that <i>PPR647</i> encodes a new chloroplast protein consisting of 11 pentratricopeptide repeat domains. Quantitative real-time PCR (qRT-PCR) assays and transcriptome analysis (RNA-seq) showed that the <i>PPR647</i> mutation significantly disrupted the expression of PEP-dependent plastid genes. In addition, RNA splicing and RNA editing of multiple chloroplast genes showed severe defects in <i>as-81647</i>. These results indicated that PPR647 is crucial for RNA editing, RNA splicing of chloroplast genes, and plays an essential role in chloroplast development.https://www.mdpi.com/1422-0067/22/20/11162pentatricopeptide repeat proteinmaizealbino-lethalRNA editingRNA splicingchloroplast |
| spellingShingle | Yan Zhao Wei Xu Yongzhong Zhang Shilei Sun Lijing Wang Shiyi Zhong Xiangyu Zhao Baoshen Liu PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize International Journal of Molecular Sciences pentatricopeptide repeat protein maize albino-lethal RNA editing RNA splicing chloroplast |
| title | PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize |
| title_full | PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize |
| title_fullStr | PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize |
| title_full_unstemmed | PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize |
| title_short | PPR647 Protein Is Required for Chloroplast RNA Editing, Splicing and Chloroplast Development in Maize |
| title_sort | ppr647 protein is required for chloroplast rna editing splicing and chloroplast development in maize |
| topic | pentatricopeptide repeat protein maize albino-lethal RNA editing RNA splicing chloroplast |
| url | https://www.mdpi.com/1422-0067/22/20/11162 |
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