Molecular identification and characterization of soybean mosaic virus isolates from Serbia
Certified and non-certified seed of the soybean cultivar Dukat showing symptoms of mottling were collected at the preharvest stage from soybean fields in the location Tamiš (South Banat District, Serbia) in 2021. To identify the presence of soybean mosaic virus (SMV), a total of 10 samples, five of...
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| Format: | Article |
| Language: | English |
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Institute of field and vegetable crops, Novi Sad
2023-01-01
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| Series: | Ratarstvo i Povrtarstvo |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2023/1821-39442301001Z.pdf |
| _version_ | 1797319603682017280 |
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| author | Zečević Katarina Stanković Ivana Drajić Teodora Delibašić Goran Krstić Branka |
| author_facet | Zečević Katarina Stanković Ivana Drajić Teodora Delibašić Goran Krstić Branka |
| author_sort | Zečević Katarina |
| collection | DOAJ |
| description | Certified and non-certified seed of the soybean cultivar Dukat showing symptoms of mottling were collected at the preharvest stage from soybean fields in the location Tamiš (South Banat District, Serbia) in 2021. To identify the presence of soybean mosaic virus (SMV), a total of 10 samples, five of each type of seed, were selected and tested by RT-PCR using primers SMVCPF/SMVCPR, which amplify a fragment of the coat protein (CP) gene. The molecular assay confirmed the presence of SMV in all tested seed samples. For further analyses, the CP and P1 genome regions of two selected isolates (DS-21 and NDS-21, representing certified and noncertified seed, respectively) were partially sequenced. The Serbian SMV isolates shared very low nucleotide diversity and were mutually closely related based on the CP (99.8% nt/99.2% aa identities) and P1 (99.7% nt/ 99.6% aa identities) regions. Phylogenetic analyses based on both genomic regions showed that the two Serbian SMV isolates originating from certified and noncertified seed belonged to one cluster, and consequently to the same SMV subpopulation. Since this is the first molecular characterization of SMV in Serbia, further research should be focused on collecting samples from different locations and of different varieties, and on determining the variability of SMV population in Serbia based on whole-genome analysis. This research will contribute to better understanding of the epidemiology of this pathogen with the ultimate goal of developing and implementing appropriate control measures. |
| first_indexed | 2024-03-08T04:09:18Z |
| format | Article |
| id | doaj.art-192cac2731a54d98af5ecd552d7827ea |
| institution | Directory Open Access Journal |
| issn | 1821-3944 2217-8392 |
| language | English |
| last_indexed | 2024-03-08T04:09:18Z |
| publishDate | 2023-01-01 |
| publisher | Institute of field and vegetable crops, Novi Sad |
| record_format | Article |
| series | Ratarstvo i Povrtarstvo |
| spelling | doaj.art-192cac2731a54d98af5ecd552d7827ea2024-02-08T22:49:07ZengInstitute of field and vegetable crops, Novi SadRatarstvo i Povrtarstvo1821-39442217-83922023-01-0160111210.5937/ratpov60-429171821-39442301001ZMolecular identification and characterization of soybean mosaic virus isolates from SerbiaZečević Katarina0https://orcid.org/0000-0003-3133-2764Stanković Ivana1https://orcid.org/0000-0003-3828-4718Drajić Teodora2Delibašić Goran3https://orcid.org/0000-0002-2024-1122Krstić Branka4University of Belgrade, Faculty of Agriculture, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaUniversity of Belgrade, Faculty of Agriculture, Belgrade, SerbiaCertified and non-certified seed of the soybean cultivar Dukat showing symptoms of mottling were collected at the preharvest stage from soybean fields in the location Tamiš (South Banat District, Serbia) in 2021. To identify the presence of soybean mosaic virus (SMV), a total of 10 samples, five of each type of seed, were selected and tested by RT-PCR using primers SMVCPF/SMVCPR, which amplify a fragment of the coat protein (CP) gene. The molecular assay confirmed the presence of SMV in all tested seed samples. For further analyses, the CP and P1 genome regions of two selected isolates (DS-21 and NDS-21, representing certified and noncertified seed, respectively) were partially sequenced. The Serbian SMV isolates shared very low nucleotide diversity and were mutually closely related based on the CP (99.8% nt/99.2% aa identities) and P1 (99.7% nt/ 99.6% aa identities) regions. Phylogenetic analyses based on both genomic regions showed that the two Serbian SMV isolates originating from certified and noncertified seed belonged to one cluster, and consequently to the same SMV subpopulation. Since this is the first molecular characterization of SMV in Serbia, further research should be focused on collecting samples from different locations and of different varieties, and on determining the variability of SMV population in Serbia based on whole-genome analysis. This research will contribute to better understanding of the epidemiology of this pathogen with the ultimate goal of developing and implementing appropriate control measures.https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2023/1821-39442301001Z.pdfcharacterizationisolatesmolecular identificationserbiasoybean mosaic virusviruses |
| spellingShingle | Zečević Katarina Stanković Ivana Drajić Teodora Delibašić Goran Krstić Branka Molecular identification and characterization of soybean mosaic virus isolates from Serbia Ratarstvo i Povrtarstvo characterization isolates molecular identification serbia soybean mosaic virus viruses |
| title | Molecular identification and characterization of soybean mosaic virus isolates from Serbia |
| title_full | Molecular identification and characterization of soybean mosaic virus isolates from Serbia |
| title_fullStr | Molecular identification and characterization of soybean mosaic virus isolates from Serbia |
| title_full_unstemmed | Molecular identification and characterization of soybean mosaic virus isolates from Serbia |
| title_short | Molecular identification and characterization of soybean mosaic virus isolates from Serbia |
| title_sort | molecular identification and characterization of soybean mosaic virus isolates from serbia |
| topic | characterization isolates molecular identification serbia soybean mosaic virus viruses |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2023/1821-39442301001Z.pdf |
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