Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants
Pathogens are among the most limiting factors for crop success and expansion. Thus, finding the underlying genetic cause of pathogen resistance is the main goal for plant geneticists. The activation of a plant’s immune system is mediated by the presence of specific receptors known as disease-resista...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/11/11/2259 |
| _version_ | 1797511541487042560 |
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| author | Andrea Fernandez-Gutierrez Juan J. Gutierrez-Gonzalez |
| author_facet | Andrea Fernandez-Gutierrez Juan J. Gutierrez-Gonzalez |
| author_sort | Andrea Fernandez-Gutierrez |
| collection | DOAJ |
| description | Pathogens are among the most limiting factors for crop success and expansion. Thus, finding the underlying genetic cause of pathogen resistance is the main goal for plant geneticists. The activation of a plant’s immune system is mediated by the presence of specific receptors known as disease-resistance genes (R genes). Typical R genes encode functional immune receptors with nucleotide-binding sites (NBS) and leucine-rich repeat (LRR) domains, making the NBS-LRRs the largest family of plant resistance genes. Establishing host resistance is crucial for plant growth and crop yield but also for reducing pesticide use. In this regard, pyramiding R genes is thought to be the most ecologically friendly way to enhance the durability of resistance. To accomplish this, researchers must first identify the related genes, or linked markers, within the genomes. However, the duplicated nature, with the presence of frequent paralogues, and clustered characteristic of NLRs make them difficult to predict with the classic automatic gene annotation pipelines. In the last several years, efforts have been made to develop new methods leading to a proliferation of reports on cloned genes. Herein, we review the bioinformatic tools to assist the discovery of R genes in plants, focusing on well-established pipelines with an important computer-based component. |
| first_indexed | 2024-03-10T05:46:46Z |
| format | Article |
| id | doaj.art-192d106e38834726b1ac17aa4bb60ee9 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:46:46Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-192d106e38834726b1ac17aa4bb60ee92023-11-22T22:02:55ZengMDPI AGAgronomy2073-43952021-11-011111225910.3390/agronomy11112259Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in PlantsAndrea Fernandez-Gutierrez0Juan J. Gutierrez-Gonzalez1Departamento de Biología Molecular, Universidad de León, 24071 León, SpainDepartamento de Biología Molecular, Universidad de León, 24071 León, SpainPathogens are among the most limiting factors for crop success and expansion. Thus, finding the underlying genetic cause of pathogen resistance is the main goal for plant geneticists. The activation of a plant’s immune system is mediated by the presence of specific receptors known as disease-resistance genes (R genes). Typical R genes encode functional immune receptors with nucleotide-binding sites (NBS) and leucine-rich repeat (LRR) domains, making the NBS-LRRs the largest family of plant resistance genes. Establishing host resistance is crucial for plant growth and crop yield but also for reducing pesticide use. In this regard, pyramiding R genes is thought to be the most ecologically friendly way to enhance the durability of resistance. To accomplish this, researchers must first identify the related genes, or linked markers, within the genomes. However, the duplicated nature, with the presence of frequent paralogues, and clustered characteristic of NLRs make them difficult to predict with the classic automatic gene annotation pipelines. In the last several years, efforts have been made to develop new methods leading to a proliferation of reports on cloned genes. Herein, we review the bioinformatic tools to assist the discovery of R genes in plants, focusing on well-established pipelines with an important computer-based component.https://www.mdpi.com/2073-4395/11/11/2259NBS-LRR genespathogen resistanceR genesNLRsgenome complexity reduction |
| spellingShingle | Andrea Fernandez-Gutierrez Juan J. Gutierrez-Gonzalez Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants Agronomy NBS-LRR genes pathogen resistance R genes NLRs genome complexity reduction |
| title | Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants |
| title_full | Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants |
| title_fullStr | Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants |
| title_full_unstemmed | Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants |
| title_short | Bioinformatic-Based Approaches for Disease-Resistance Gene Discovery in Plants |
| title_sort | bioinformatic based approaches for disease resistance gene discovery in plants |
| topic | NBS-LRR genes pathogen resistance R genes NLRs genome complexity reduction |
| url | https://www.mdpi.com/2073-4395/11/11/2259 |
| work_keys_str_mv | AT andreafernandezgutierrez bioinformaticbasedapproachesfordiseaseresistancegenediscoveryinplants AT juanjgutierrezgonzalez bioinformaticbasedapproachesfordiseaseresistancegenediscoveryinplants |