Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses
Abstract To evaluate the degree of postmating isolation and the evolutionary relationships among frog species in the genus Fejervarya from Indonesia (Lesser Sunda), Bangladesh, China, and Japan, crossing experiments and molecular phylogenetic analyses were carried out. Crossing experiments revealed...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-10-01
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| Series: | Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ece3.9436 |
| _version_ | 1811316320110641152 |
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| author | Mahmudul Hasan Nia Kurniawan Aris Soewondo Wilmientje Marlene Mesang Nalley Masafumi Matsui Takeshi Igawa Masayuki Sumida |
| author_facet | Mahmudul Hasan Nia Kurniawan Aris Soewondo Wilmientje Marlene Mesang Nalley Masafumi Matsui Takeshi Igawa Masayuki Sumida |
| author_sort | Mahmudul Hasan |
| collection | DOAJ |
| description | Abstract To evaluate the degree of postmating isolation and the evolutionary relationships among frog species in the genus Fejervarya from Indonesia (Lesser Sunda), Bangladesh, China, and Japan, crossing experiments and molecular phylogenetic analyses were carried out. Crossing experiments revealed that reciprocal hybrids among F. iskandari, F. verruculosa, and F. sp. large type and between F. multistriata and F. kawamurai are viable through metamorphosis, while those between the F. iskandari group and F. limnocharis group were completely or partially inviable at the tadpole stage and those between Southeast Asian and South Asian Fejervarya groups were completely inviable at the embryonic stage. The mature reciprocal hybrids between F. iskandari and F. verruculosa from Lesser Sunda, Indonesia, showed some degree of abnormality in spermatogenesis. In phylogenetic analyses based on mtDNA Cytb sequences, F. iskandari formed a sister clade with F. verruculosa from Lesser Sunda, Indonesia, with 8.1% sequence divergence. F. multistriata from China formed a clade with populations of F. limnocharis in Thailand, Malaysia, and Indonesia (topotype), and these taxa showed sister relationships to F. kawamurai from Japan with 8.9% sequence divergence. Fejervarya sp. small type from Bangladesh formed a clade with the other South Asian members of the Fejervarya group and formed a sister clade with the Southeast Asian Fejervarya group, with 23.1% sequence divergence in the Cytb gene. These results showed that the degree of postmating isolation reflects molecular phylogenetic relationships and that F. iskandari and F. verruculosa from Indonesia (Lesser Sunda) are reproductively isolated by abnormalities in spermatogenesis and show genetic differentiation. |
| first_indexed | 2024-04-13T11:46:53Z |
| format | Article |
| id | doaj.art-192dfd2f9d8d43debb2d255fe8107e02 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-13T11:46:53Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-192dfd2f9d8d43debb2d255fe8107e022022-12-22T02:48:09ZengWileyEcology and Evolution2045-77582022-10-011210n/an/a10.1002/ece3.9436Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analysesMahmudul Hasan0Nia Kurniawan1Aris Soewondo2Wilmientje Marlene Mesang Nalley3Masafumi Matsui4Takeshi Igawa5Masayuki Sumida6Department of Fisheries Bangamata Sheikh Fojilatunnesa Mujib Science and Technology University Jamalpur BangladeshAmphibian Research Center Hiroshima University Higashihiroshima JapanDepartment of Biology, Faculty of Mathematics and Natural Sciences Brawijaya University Malang East Java IndonesiaFaculty of Animal Science University of Nusa Cendana Kupang IndonesiaGraduate School of Human and Environmental Studies Kyoto University Kyoto JapanAmphibian Research Center Hiroshima University Higashihiroshima JapanAmphibian Research Center Hiroshima University Higashihiroshima JapanAbstract To evaluate the degree of postmating isolation and the evolutionary relationships among frog species in the genus Fejervarya from Indonesia (Lesser Sunda), Bangladesh, China, and Japan, crossing experiments and molecular phylogenetic analyses were carried out. Crossing experiments revealed that reciprocal hybrids among F. iskandari, F. verruculosa, and F. sp. large type and between F. multistriata and F. kawamurai are viable through metamorphosis, while those between the F. iskandari group and F. limnocharis group were completely or partially inviable at the tadpole stage and those between Southeast Asian and South Asian Fejervarya groups were completely inviable at the embryonic stage. The mature reciprocal hybrids between F. iskandari and F. verruculosa from Lesser Sunda, Indonesia, showed some degree of abnormality in spermatogenesis. In phylogenetic analyses based on mtDNA Cytb sequences, F. iskandari formed a sister clade with F. verruculosa from Lesser Sunda, Indonesia, with 8.1% sequence divergence. F. multistriata from China formed a clade with populations of F. limnocharis in Thailand, Malaysia, and Indonesia (topotype), and these taxa showed sister relationships to F. kawamurai from Japan with 8.9% sequence divergence. Fejervarya sp. small type from Bangladesh formed a clade with the other South Asian members of the Fejervarya group and formed a sister clade with the Southeast Asian Fejervarya group, with 23.1% sequence divergence in the Cytb gene. These results showed that the degree of postmating isolation reflects molecular phylogenetic relationships and that F. iskandari and F. verruculosa from Indonesia (Lesser Sunda) are reproductively isolated by abnormalities in spermatogenesis and show genetic differentiation.https://doi.org/10.1002/ece3.9436FejervaryamtDNAreproductive/Postmating isolationspecies |
| spellingShingle | Mahmudul Hasan Nia Kurniawan Aris Soewondo Wilmientje Marlene Mesang Nalley Masafumi Matsui Takeshi Igawa Masayuki Sumida Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses Ecology and Evolution Fejervarya mtDNA reproductive/Postmating isolation species |
| title | Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses |
| title_full | Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses |
| title_fullStr | Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses |
| title_full_unstemmed | Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses |
| title_short | Postmating isolation and evolutionary relationships among Fejervarya species from Lesser Sunda, Indonesia and other Asian countries revealed by crossing experiments and mtDNA Cytb sequence analyses |
| title_sort | postmating isolation and evolutionary relationships among fejervarya species from lesser sunda indonesia and other asian countries revealed by crossing experiments and mtdna cytb sequence analyses |
| topic | Fejervarya mtDNA reproductive/Postmating isolation species |
| url | https://doi.org/10.1002/ece3.9436 |
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