Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae
Cellulite is associated with a complex array of adipocytes under the skin and vascular system. A herbal compress that was previously developed was proven to have an anti-cellulite effect in healthy volunteers within 2 weeks of treatment. However, its mechanism and ingredients responsible for reducin...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/14/3/253 |
| _version_ | 1797541736648540160 |
|---|---|
| author | Ngamrayu Ngamdokmai Tamkeen Urooj Paracha Neti Waranuch Krongkarn Chootip Wudtichai Wisuitiprot Nungruthai Suphrom Kamonlak Insumrong Kornkanok Ingkaninan |
| author_facet | Ngamrayu Ngamdokmai Tamkeen Urooj Paracha Neti Waranuch Krongkarn Chootip Wudtichai Wisuitiprot Nungruthai Suphrom Kamonlak Insumrong Kornkanok Ingkaninan |
| author_sort | Ngamrayu Ngamdokmai |
| collection | DOAJ |
| description | Cellulite is associated with a complex array of adipocytes under the skin and vascular system. A herbal compress that was previously developed was proven to have an anti-cellulite effect in healthy volunteers within 2 weeks of treatment. However, its mechanism and ingredients responsible for reducing cellulite were not known. The purpose of this study was to investigate the activity of eight essential oils in, and two water extracts from, the ingredients of the herbal compress together with nine monoterpenoid constituents on the 3T3-L1 adipocytes. The vasodilatory effect on rat aortae was also studied. The adipocytes were induced by dexamethasone, 3-isobutyl-1-methylxanthine and insulin. At all concentrations tested, all essential oils, water extracts and their monoterpenoid constituents significantly inhibited lipid accumulation activity (<i>p</i> < 0.05) and decreased the amount of triglycerides when compared to untreated cells (<i>p</i> < 0.01). In addition, our results showed that the mixed oil distilled from the herbal compress mixed ingredients could relax the isolated rat aorta (EC<sub>50</sub> = 14.74 ± 2.65 µg/mL). In conclusion, all essential oils, extracts and chemical constituents tested showed effects on adipogenesis inhibition and lipolysis induction on the cultured adipocytes with the mixed oil demonstrating vasorelaxation activity, all of which might be the mechanisms of the anti-cellulite effects of the herbal compress. |
| first_indexed | 2024-03-10T13:20:00Z |
| format | Article |
| id | doaj.art-1930c833a1ad4033b6839630679735d3 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T13:20:00Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-1930c833a1ad4033b6839630679735d32023-11-21T10:05:56ZengMDPI AGPharmaceuticals1424-82472021-03-0114325310.3390/ph14030253Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat AortaeNgamrayu Ngamdokmai0Tamkeen Urooj Paracha1Neti Waranuch2Krongkarn Chootip3Wudtichai Wisuitiprot4Nungruthai Suphrom5Kamonlak Insumrong6Kornkanok Ingkaninan7Centre of Excellence in Cannabis Research, Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, ThailandDepartment of Pharmacology, Faculty of Pharmacy, Hamdard University, Main Campus, Karachi 74600, PakistanCosmetics and Natural Products Research Center and Center of Excellence for Innovation in Chemistry, Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Naresuan University, Phitsanulok 65000, ThailandDepartment of Physiology, Faculty of Medical Sciences, Naresuan University, Phitsanulok 65000, ThailandDepartment of Thai Traditional Medicine, Sirindhorn College of Public Health, Phitsanulok 65130, ThailandDepartment of Chemistry, Faculty of Science and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, ThailandDepartment of Chemistry, Faculty of Science and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, ThailandCentre of Excellence in Cannabis Research, Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, ThailandCellulite is associated with a complex array of adipocytes under the skin and vascular system. A herbal compress that was previously developed was proven to have an anti-cellulite effect in healthy volunteers within 2 weeks of treatment. However, its mechanism and ingredients responsible for reducing cellulite were not known. The purpose of this study was to investigate the activity of eight essential oils in, and two water extracts from, the ingredients of the herbal compress together with nine monoterpenoid constituents on the 3T3-L1 adipocytes. The vasodilatory effect on rat aortae was also studied. The adipocytes were induced by dexamethasone, 3-isobutyl-1-methylxanthine and insulin. At all concentrations tested, all essential oils, water extracts and their monoterpenoid constituents significantly inhibited lipid accumulation activity (<i>p</i> < 0.05) and decreased the amount of triglycerides when compared to untreated cells (<i>p</i> < 0.01). In addition, our results showed that the mixed oil distilled from the herbal compress mixed ingredients could relax the isolated rat aorta (EC<sub>50</sub> = 14.74 ± 2.65 µg/mL). In conclusion, all essential oils, extracts and chemical constituents tested showed effects on adipogenesis inhibition and lipolysis induction on the cultured adipocytes with the mixed oil demonstrating vasorelaxation activity, all of which might be the mechanisms of the anti-cellulite effects of the herbal compress.https://www.mdpi.com/1424-8247/14/3/253celluliteessential oilmonoterpenesThai herbal compressadipogenesislipolysis |
| spellingShingle | Ngamrayu Ngamdokmai Tamkeen Urooj Paracha Neti Waranuch Krongkarn Chootip Wudtichai Wisuitiprot Nungruthai Suphrom Kamonlak Insumrong Kornkanok Ingkaninan Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae Pharmaceuticals cellulite essential oil monoterpenes Thai herbal compress adipogenesis lipolysis |
| title | Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae |
| title_full | Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae |
| title_fullStr | Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae |
| title_full_unstemmed | Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae |
| title_short | Effects of Essential Oils and Some Constituents from Ingredients of Anti-Cellulite Herbal Compress on 3T3-L1 Adipocytes and Rat Aortae |
| title_sort | effects of essential oils and some constituents from ingredients of anti cellulite herbal compress on 3t3 l1 adipocytes and rat aortae |
| topic | cellulite essential oil monoterpenes Thai herbal compress adipogenesis lipolysis |
| url | https://www.mdpi.com/1424-8247/14/3/253 |
| work_keys_str_mv | AT ngamrayungamdokmai effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT tamkeenuroojparacha effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT netiwaranuch effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT krongkarnchootip effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT wudtichaiwisuitiprot effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT nungruthaisuphrom effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT kamonlakinsumrong effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae AT kornkanokingkaninan effectsofessentialoilsandsomeconstituentsfromingredientsofanticelluliteherbalcompresson3t3l1adipocytesandrataortae |