Isochoric Supercooling Organ Preservation System
This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/8/934 |
| _version_ | 1797585433495863296 |
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| author | Gabriel Năstase Florin Botea George-Andrei Beșchea Ștefan-Ioan Câmpean Alexandru Barcu Ion Neacșu Vlad Herlea Irinel Popescu Tammy T. Chang Boris Rubinsky Alexandru Șerban |
| author_facet | Gabriel Năstase Florin Botea George-Andrei Beșchea Ștefan-Ioan Câmpean Alexandru Barcu Ion Neacșu Vlad Herlea Irinel Popescu Tammy T. Chang Boris Rubinsky Alexandru Șerban |
| author_sort | Gabriel Năstase |
| collection | DOAJ |
| description | This technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures without the need for cryoprotectant additives. Detailed technical designs and usage protocols are provided for researchers interested in exploring this field. The paper also presents a control system based on the thermodynamics of isochoric freezing, utilizing pressure monitoring for process control. Sham experiments were performed using whole pig liver sourced from a local food supplier to evaluate the system’s ability to sustain supercooling without ice nucleation for extended periods. The results demonstrated sustained supercooling without ice nucleation in pig liver tissue for 24 and 48 h. These findings suggest the potential of this technology for large-volume, cryoprotectant-free organ preservation with real-time control over the preservation process. The simplicity of the isochoric supercooling device and the design details provided in the paper are expected to serve as encouragement for other researchers in the field to pursue further research on isochoric supercooling. However, final evidence that these preserved organs can be successfully transplanted is still lacking. |
| first_indexed | 2024-03-11T00:06:07Z |
| format | Article |
| id | doaj.art-193223a138c7451495e288d75636920d |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-11T00:06:07Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-193223a138c7451495e288d75636920d2023-11-19T00:18:03ZengMDPI AGBioengineering2306-53542023-08-0110893410.3390/bioengineering10080934Isochoric Supercooling Organ Preservation SystemGabriel Năstase0Florin Botea1George-Andrei Beșchea2Ștefan-Ioan Câmpean3Alexandru Barcu4Ion Neacșu5Vlad Herlea6Irinel Popescu7Tammy T. Chang8Boris Rubinsky9Alexandru Șerban10Department of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, RomaniaCenter of Excellence in Translational Medicine CEMT, “Dan Setlacec” Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, RomaniaDepartment of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, RomaniaDepartment of Building Services, Faculty of Civil Engineering, Transilvania University of Brasov, 500152 Brasov, RomaniaCenter of Excellence in Translational Medicine CEMT, “Dan Setlacec” Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, RomaniaCriomec SA, 800219 Galati, RomaniaCenter of Excellence in Translational Medicine CEMT, “Dan Setlacec” Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, RomaniaCenter of Excellence in Translational Medicine CEMT, “Dan Setlacec” Center of General Surgery and Liver Transplantation, Fundeni Clinical Institute, 022328 Bucharest, RomaniaDepartment of Surgery, University of California, San Francisco, CA 94143, USADepartment of Mechanical Engineering, University of California, Berkeley, CA 94720, USADepartment of Thermotechnics, Engines, Thermal and Refrigeration Equipment, Faculty of Mechanical Engineering and Mechatronics, University Politehnica of Bucharest, 060042 Bucharest, RomaniaThis technical paper introduces a novel organ preservation system based on isochoric (constant volume) supercooling. The system is designed to enhance the stability of the metastable supercooling state, offering potential long-term preservation of large biological organs at subfreezing temperatures without the need for cryoprotectant additives. Detailed technical designs and usage protocols are provided for researchers interested in exploring this field. The paper also presents a control system based on the thermodynamics of isochoric freezing, utilizing pressure monitoring for process control. Sham experiments were performed using whole pig liver sourced from a local food supplier to evaluate the system’s ability to sustain supercooling without ice nucleation for extended periods. The results demonstrated sustained supercooling without ice nucleation in pig liver tissue for 24 and 48 h. These findings suggest the potential of this technology for large-volume, cryoprotectant-free organ preservation with real-time control over the preservation process. The simplicity of the isochoric supercooling device and the design details provided in the paper are expected to serve as encouragement for other researchers in the field to pursue further research on isochoric supercooling. However, final evidence that these preserved organs can be successfully transplanted is still lacking.https://www.mdpi.com/2306-5354/10/8/934isochoric systemsubfreezing temperatureslarge organ preservationcryopreservationsupercoolingliver |
| spellingShingle | Gabriel Năstase Florin Botea George-Andrei Beșchea Ștefan-Ioan Câmpean Alexandru Barcu Ion Neacșu Vlad Herlea Irinel Popescu Tammy T. Chang Boris Rubinsky Alexandru Șerban Isochoric Supercooling Organ Preservation System Bioengineering isochoric system subfreezing temperatures large organ preservation cryopreservation supercooling liver |
| title | Isochoric Supercooling Organ Preservation System |
| title_full | Isochoric Supercooling Organ Preservation System |
| title_fullStr | Isochoric Supercooling Organ Preservation System |
| title_full_unstemmed | Isochoric Supercooling Organ Preservation System |
| title_short | Isochoric Supercooling Organ Preservation System |
| title_sort | isochoric supercooling organ preservation system |
| topic | isochoric system subfreezing temperatures large organ preservation cryopreservation supercooling liver |
| url | https://www.mdpi.com/2306-5354/10/8/934 |
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