Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma
The detection of epidermal growth factor receptor (<i>EGFR</i>) mutation, based on tissue biopsy samples, provides a valuable guideline for the prognosis and precision medicine in patients with lung cancer. In this study, we aimed to examine minimally invasive bronchial washing (BW)-deri...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/12/10/2822 |
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| author | Juhee Park Chaeeun Lee Jung Seop Eom Mi-Hyun Kim Yoon-Kyoung Cho |
| author_facet | Juhee Park Chaeeun Lee Jung Seop Eom Mi-Hyun Kim Yoon-Kyoung Cho |
| author_sort | Juhee Park |
| collection | DOAJ |
| description | The detection of epidermal growth factor receptor (<i>EGFR</i>) mutation, based on tissue biopsy samples, provides a valuable guideline for the prognosis and precision medicine in patients with lung cancer. In this study, we aimed to examine minimally invasive bronchial washing (BW)-derived extracellular vesicles (EVs) for <i>EGFR</i> mutation analysis in patients with lung cancer. A lab-on-a-disc equipped with a filter with 20-nm pore diameter, Exo-Disc, was used to enrich EVs in BW samples. The overall detection sensitivity of <i>EGFR</i> mutations in 55 BW-derived samples was 89.7% and 31.0% for EV-derived DNA (EV-DNA) and EV-excluded cell free-DNA (EV-X-cfDNA), respectively, with 100% specificity. The detection rate of T790M in 13 matched samples was 61.5%, 10.0%, and 30.8% from BW-derived EV-DNA, plasma-derived cfDNA, and tissue samples, respectively. The acquisition of T790M resistance mutation was detected earlier in BW-derived EVs than plasma or tissue samples. The longitudinal analysis of BW-derived EVs showed excellent correlation with the disease progression measured by CT images. The <i>EGFR</i> mutations can be readily detected in BW-derived EVs, which demonstrates their clinical potential as a liquid-biopsy sample that may aid precise management, including assessment of the treatment response and drug resistance in patients with lung cancer. |
| first_indexed | 2024-03-10T15:55:35Z |
| format | Article |
| id | doaj.art-19366d0d76b5443a8196852aa08845c2 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T15:55:35Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-19366d0d76b5443a8196852aa08845c22023-11-20T15:38:46ZengMDPI AGCancers2072-66942020-09-011210282210.3390/cancers12102822Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung CarcinomaJuhee Park0Chaeeun Lee1Jung Seop Eom2Mi-Hyun Kim3Yoon-Kyoung Cho4Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, KoreaCenter for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, KoreaDepartment of Internal Medicine, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, KoreaDepartment of Internal Medicine, Pusan National University School of Medicine and Biomedical Research Institute, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, KoreaCenter for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan 44919, KoreaThe detection of epidermal growth factor receptor (<i>EGFR</i>) mutation, based on tissue biopsy samples, provides a valuable guideline for the prognosis and precision medicine in patients with lung cancer. In this study, we aimed to examine minimally invasive bronchial washing (BW)-derived extracellular vesicles (EVs) for <i>EGFR</i> mutation analysis in patients with lung cancer. A lab-on-a-disc equipped with a filter with 20-nm pore diameter, Exo-Disc, was used to enrich EVs in BW samples. The overall detection sensitivity of <i>EGFR</i> mutations in 55 BW-derived samples was 89.7% and 31.0% for EV-derived DNA (EV-DNA) and EV-excluded cell free-DNA (EV-X-cfDNA), respectively, with 100% specificity. The detection rate of T790M in 13 matched samples was 61.5%, 10.0%, and 30.8% from BW-derived EV-DNA, plasma-derived cfDNA, and tissue samples, respectively. The acquisition of T790M resistance mutation was detected earlier in BW-derived EVs than plasma or tissue samples. The longitudinal analysis of BW-derived EVs showed excellent correlation with the disease progression measured by CT images. The <i>EGFR</i> mutations can be readily detected in BW-derived EVs, which demonstrates their clinical potential as a liquid-biopsy sample that may aid precise management, including assessment of the treatment response and drug resistance in patients with lung cancer.https://www.mdpi.com/2072-6694/12/10/2822lung cancerliquid biopsybronchial washing (BW)extracellular vesiclesEGFRT790M |
| spellingShingle | Juhee Park Chaeeun Lee Jung Seop Eom Mi-Hyun Kim Yoon-Kyoung Cho Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma Cancers lung cancer liquid biopsy bronchial washing (BW) extracellular vesicles EGFR T790M |
| title | Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma |
| title_full | Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma |
| title_fullStr | Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma |
| title_full_unstemmed | Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma |
| title_short | Detection of <i>EGFR</i> Mutations Using Bronchial Washing-Derived Extracellular Vesicles in Patients with Non-Small-Cell Lung Carcinoma |
| title_sort | detection of i egfr i mutations using bronchial washing derived extracellular vesicles in patients with non small cell lung carcinoma |
| topic | lung cancer liquid biopsy bronchial washing (BW) extracellular vesicles EGFR T790M |
| url | https://www.mdpi.com/2072-6694/12/10/2822 |
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