Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance
Salt stress is an important environmental factor limiting plant growth and crop production. Plant adaptation to salt stress can be improved by chemical pretreatment. This study aims to identify whether hydrogen peroxide (H2O2) pretreatment of seedlings affects the stress tolerance of Arabidopsis tha...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-04-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.866063/full |
| _version_ | 1828272444360622080 |
|---|---|
| author | Qikun Zhang Xiuru Dai Huanpeng Wang Fanhua Wang Dongxue Tang Chunyun Jiang Chunyun Jiang Xiaoyan Zhang Wenjing Guo Yuanyuan Lei Changle Ma Hui Zhang Pinghua Li Yanxiu Zhao Zenglan Wang |
| author_facet | Qikun Zhang Xiuru Dai Huanpeng Wang Fanhua Wang Dongxue Tang Chunyun Jiang Chunyun Jiang Xiaoyan Zhang Wenjing Guo Yuanyuan Lei Changle Ma Hui Zhang Pinghua Li Yanxiu Zhao Zenglan Wang |
| author_sort | Qikun Zhang |
| collection | DOAJ |
| description | Salt stress is an important environmental factor limiting plant growth and crop production. Plant adaptation to salt stress can be improved by chemical pretreatment. This study aims to identify whether hydrogen peroxide (H2O2) pretreatment of seedlings affects the stress tolerance of Arabidopsis thaliana seedlings. The results show that pretreatment with H2O2 at appropriate concentrations enhances the salt tolerance ability of Arabidopsis seedlings, as revealed by lower Na+ levels, greater K+ levels, and improved K+/Na+ ratios in leaves. Furthermore, H2O2 pretreatment improves the membrane properties by reducing the relative membrane permeability (RMP) and malonaldehyde (MDA) content in addition to improving the activities of antioxidant enzymes, including superoxide dismutase, and glutathione peroxidase. Our transcription data show that exogenous H2O2 pretreatment leads to the induced expression of cell cycle, redox regulation, and cell wall organization-related genes in Arabidopsis, which may accelerate cell proliferation, enhance tolerance to osmotic stress, maintain the redox balance, and remodel the cell walls of plants in subsequent high-salt environments. |
| first_indexed | 2024-04-13T06:07:41Z |
| format | Article |
| id | doaj.art-1936d1475eb1441493fe9f508a622af7 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T06:07:41Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1936d1475eb1441493fe9f508a622af72022-12-22T02:59:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.866063866063Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt ToleranceQikun Zhang0Xiuru Dai1Huanpeng Wang2Fanhua Wang3Dongxue Tang4Chunyun Jiang5Chunyun Jiang6Xiaoyan Zhang7Wenjing Guo8Yuanyuan Lei9Changle Ma10Hui Zhang11Pinghua Li12Yanxiu Zhao13Zenglan Wang14Shandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaState Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaLinyi Center for Disease Control and Prevention, Linyi, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaState Key Laboratory of Crop Biology, College of Agronomic Sciences, Shandong Agricultural University, Tai’an, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Plant Stress, College of Life Sciences, Shandong Normal University, Jinan, ChinaSalt stress is an important environmental factor limiting plant growth and crop production. Plant adaptation to salt stress can be improved by chemical pretreatment. This study aims to identify whether hydrogen peroxide (H2O2) pretreatment of seedlings affects the stress tolerance of Arabidopsis thaliana seedlings. The results show that pretreatment with H2O2 at appropriate concentrations enhances the salt tolerance ability of Arabidopsis seedlings, as revealed by lower Na+ levels, greater K+ levels, and improved K+/Na+ ratios in leaves. Furthermore, H2O2 pretreatment improves the membrane properties by reducing the relative membrane permeability (RMP) and malonaldehyde (MDA) content in addition to improving the activities of antioxidant enzymes, including superoxide dismutase, and glutathione peroxidase. Our transcription data show that exogenous H2O2 pretreatment leads to the induced expression of cell cycle, redox regulation, and cell wall organization-related genes in Arabidopsis, which may accelerate cell proliferation, enhance tolerance to osmotic stress, maintain the redox balance, and remodel the cell walls of plants in subsequent high-salt environments.https://www.frontiersin.org/articles/10.3389/fpls.2022.866063/fullhydrogen peroxidepretreatmentArabidopsis thalianasalt stresstranscriptome profiling |
| spellingShingle | Qikun Zhang Xiuru Dai Huanpeng Wang Fanhua Wang Dongxue Tang Chunyun Jiang Chunyun Jiang Xiaoyan Zhang Wenjing Guo Yuanyuan Lei Changle Ma Hui Zhang Pinghua Li Yanxiu Zhao Zenglan Wang Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance Frontiers in Plant Science hydrogen peroxide pretreatment Arabidopsis thaliana salt stress transcriptome profiling |
| title | Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance |
| title_full | Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance |
| title_fullStr | Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance |
| title_full_unstemmed | Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance |
| title_short | Transcriptomic Profiling Provides Molecular Insights Into Hydrogen Peroxide-Enhanced Arabidopsis Growth and Its Salt Tolerance |
| title_sort | transcriptomic profiling provides molecular insights into hydrogen peroxide enhanced arabidopsis growth and its salt tolerance |
| topic | hydrogen peroxide pretreatment Arabidopsis thaliana salt stress transcriptome profiling |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.866063/full |
| work_keys_str_mv | AT qikunzhang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT xiurudai transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT huanpengwang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT fanhuawang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT dongxuetang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT chunyunjiang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT chunyunjiang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT xiaoyanzhang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT wenjingguo transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT yuanyuanlei transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT changlema transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT huizhang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT pinghuali transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT yanxiuzhao transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance AT zenglanwang transcriptomicprofilingprovidesmolecularinsightsintohydrogenperoxideenhancedarabidopsisgrowthanditssalttolerance |