Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia
Biochemical and biological differences in the venom of <i>Crotalus durissus cumanensis</i> from three ecoregions of Colombia were evaluated. Rattlesnakes were collected from the geographic areas of Magdalena Medio (MM), Caribe (CA) and Orinoquía (OR). All three regionally distributed ven...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/8/532 |
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| author | Ariadna Rodríguez-Vargas Nohora Vega Edgar Reyes-Montaño Gerardo Corzo Edgar Neri-Castro Herlinda Clement Francisco Ruiz-Gómez |
| author_facet | Ariadna Rodríguez-Vargas Nohora Vega Edgar Reyes-Montaño Gerardo Corzo Edgar Neri-Castro Herlinda Clement Francisco Ruiz-Gómez |
| author_sort | Ariadna Rodríguez-Vargas |
| collection | DOAJ |
| description | Biochemical and biological differences in the venom of <i>Crotalus durissus cumanensis</i> from three ecoregions of Colombia were evaluated. Rattlesnakes were collected from the geographic areas of Magdalena Medio (MM), Caribe (CA) and Orinoquía (OR). All three regionally distributed venoms contain proteases, PLA<sub>2</sub>s and the basic subunit of crotoxin. However, only crotamine was detected in the CA venom. The highest lethality, coagulant, phospholipase A<sub>2</sub> and hyaluronidase activities were found in the MM venom. Also, some differences, observed by western blot and immunoaffinity, were found in all three venoms when using commercial antivenoms. Furthermore, all three eco-regional venoms showed intraspecific variability, considering the differences in the abundance and intensity of their components, in addition to the activity and response to commercial antivenoms. |
| first_indexed | 2024-03-09T12:25:27Z |
| format | Article |
| id | doaj.art-193aae0b85784c20abd11527db6ee329 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T12:25:27Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-193aae0b85784c20abd11527db6ee3292023-11-30T22:35:39ZengMDPI AGToxins2072-66512022-08-0114853210.3390/toxins14080532Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from ColombiaAriadna Rodríguez-Vargas0Nohora Vega1Edgar Reyes-Montaño2Gerardo Corzo3Edgar Neri-Castro4Herlinda Clement5Francisco Ruiz-Gómez6Grupo de Investigación en Proteínas, Universidad Nacional de Colombia, Bogotá 11001, ColombiaGrupo de Investigación en Proteínas, Universidad Nacional de Colombia, Bogotá 11001, ColombiaGrupo de Investigación en Proteínas, Universidad Nacional de Colombia, Bogotá 11001, ColombiaDepartamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoDepartamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoDepartamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca 62210, MexicoGrupo de Investigación en Animales Ponzoñosos y sus Venenos, Instituto Nacional de Salud, Bogotá 111321, ColombiaBiochemical and biological differences in the venom of <i>Crotalus durissus cumanensis</i> from three ecoregions of Colombia were evaluated. Rattlesnakes were collected from the geographic areas of Magdalena Medio (MM), Caribe (CA) and Orinoquía (OR). All three regionally distributed venoms contain proteases, PLA<sub>2</sub>s and the basic subunit of crotoxin. However, only crotamine was detected in the CA venom. The highest lethality, coagulant, phospholipase A<sub>2</sub> and hyaluronidase activities were found in the MM venom. Also, some differences, observed by western blot and immunoaffinity, were found in all three venoms when using commercial antivenoms. Furthermore, all three eco-regional venoms showed intraspecific variability, considering the differences in the abundance and intensity of their components, in addition to the activity and response to commercial antivenoms.https://www.mdpi.com/2072-6651/14/8/532<i>Crotalus durissus cumanensis</i>venomantivenom |
| spellingShingle | Ariadna Rodríguez-Vargas Nohora Vega Edgar Reyes-Montaño Gerardo Corzo Edgar Neri-Castro Herlinda Clement Francisco Ruiz-Gómez Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia Toxins <i>Crotalus durissus cumanensis</i> venom antivenom |
| title | Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia |
| title_full | Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia |
| title_fullStr | Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia |
| title_full_unstemmed | Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia |
| title_short | Intraspecific Differences in the Venom of <i>Crotalus durissus cumanensis</i> from Colombia |
| title_sort | intraspecific differences in the venom of i crotalus durissus cumanensis i from colombia |
| topic | <i>Crotalus durissus cumanensis</i> venom antivenom |
| url | https://www.mdpi.com/2072-6651/14/8/532 |
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