CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations
Connections between vitamin D and psoriasis have been a matter of interest for the past decades, with its active metabolite, 1,25(OH)<sub>2</sub> vitamin D, being valued for antiproliferative and immunomodulatory effects. However, none of vitamin D’s actions could be possible without the...
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MDPI AG
2023-12-01
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| author | Iulia-Alexandra Paliu Maria-Victoria Olinca Simona-Laura Ianosi Claudia-Valentina Georgescu Adina Turcu-Stiolica Magdalena Diaconu Cristiana-Iulia Dumitrescu Andrei-Adrian Tica |
| author_facet | Iulia-Alexandra Paliu Maria-Victoria Olinca Simona-Laura Ianosi Claudia-Valentina Georgescu Adina Turcu-Stiolica Magdalena Diaconu Cristiana-Iulia Dumitrescu Andrei-Adrian Tica |
| author_sort | Iulia-Alexandra Paliu |
| collection | DOAJ |
| description | Connections between vitamin D and psoriasis have been a matter of interest for the past decades, with its active metabolite, 1,25(OH)<sub>2</sub> vitamin D, being valued for antiproliferative and immunomodulatory effects. However, none of vitamin D’s actions could be possible without the CYP27B1 enzyme that bio-activates this metabolite of interest. In order to see if there is any link between the enzyme expression and the disease’s particularities, we conducted a preliminary study that involved 11 skin biopsies of patients with mild (n = 4) or moderate to severe psoriasis (n = 7). The cell proliferation antigen Ki67 and the CD45RO+ marker were also assessed. Compared with healthy skin, in psoriasis, it is reported that the enzyme’s expression seems to be more ubiquitous, but a clear correlation between the disease’s severity and the CYP27B1 expression was, to our knowledge, lacking. We found that, in patients with very mild psoriasis, the enzyme expression was observed in the epidermal stratum basale in a similar manner as in healthy skin specimens. Contrary, for higher severity scores, a divergent result was observed, with the enzyme being either variably spread in the epidermal stratum spinosum or completely absent. Unlike malignant diseases, a significant connection between CYP27B1 and Ki67 (<i>p</i> = 0.313) or CYP27B1 and CD45RO+ (<i>p</i> = 0.657) does not seem to be relevant in psoriasis. |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T10:44:01Z |
| publishDate | 2023-12-01 |
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| record_format | Article |
| series | Life |
| spelling | doaj.art-193b2f51f61d40c5ae9ff2ddbd151aba2024-01-26T17:20:23ZengMDPI AGLife2075-17292023-12-011411510.3390/life14010015CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical ObservationsIulia-Alexandra Paliu0Maria-Victoria Olinca1Simona-Laura Ianosi2Claudia-Valentina Georgescu3Adina Turcu-Stiolica4Magdalena Diaconu5Cristiana-Iulia Dumitrescu6Andrei-Adrian Tica7Department of Pharmacology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Pathology, “Carol Davila” University of Medicine and Pharmacy, 020021 Bucharest, RomaniaDepartment of Dermatology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Pathology, Clinical Emergency County Hospital of Craiova, 200349 Craiova, RomaniaDepartment of Pharmacoeconomics, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Pharmacology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Pharmacology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaDepartment of Pharmacology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, RomaniaConnections between vitamin D and psoriasis have been a matter of interest for the past decades, with its active metabolite, 1,25(OH)<sub>2</sub> vitamin D, being valued for antiproliferative and immunomodulatory effects. However, none of vitamin D’s actions could be possible without the CYP27B1 enzyme that bio-activates this metabolite of interest. In order to see if there is any link between the enzyme expression and the disease’s particularities, we conducted a preliminary study that involved 11 skin biopsies of patients with mild (n = 4) or moderate to severe psoriasis (n = 7). The cell proliferation antigen Ki67 and the CD45RO+ marker were also assessed. Compared with healthy skin, in psoriasis, it is reported that the enzyme’s expression seems to be more ubiquitous, but a clear correlation between the disease’s severity and the CYP27B1 expression was, to our knowledge, lacking. We found that, in patients with very mild psoriasis, the enzyme expression was observed in the epidermal stratum basale in a similar manner as in healthy skin specimens. Contrary, for higher severity scores, a divergent result was observed, with the enzyme being either variably spread in the epidermal stratum spinosum or completely absent. Unlike malignant diseases, a significant connection between CYP27B1 and Ki67 (<i>p</i> = 0.313) or CYP27B1 and CD45RO+ (<i>p</i> = 0.657) does not seem to be relevant in psoriasis.https://www.mdpi.com/2075-1729/14/1/151-α-hydroxylaseCYP27B1 enzymevitamin Dpsoriasis |
| spellingShingle | Iulia-Alexandra Paliu Maria-Victoria Olinca Simona-Laura Ianosi Claudia-Valentina Georgescu Adina Turcu-Stiolica Magdalena Diaconu Cristiana-Iulia Dumitrescu Andrei-Adrian Tica CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations Life 1-α-hydroxylase CYP27B1 enzyme vitamin D psoriasis |
| title | CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations |
| title_full | CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations |
| title_fullStr | CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations |
| title_full_unstemmed | CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations |
| title_short | CYP27B1 Enzyme in Psoriasis: A Preliminary Study of Immunohistochemical Observations |
| title_sort | cyp27b1 enzyme in psoriasis a preliminary study of immunohistochemical observations |
| topic | 1-α-hydroxylase CYP27B1 enzyme vitamin D psoriasis |
| url | https://www.mdpi.com/2075-1729/14/1/15 |
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