Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus
African swine fever virus (ASFV) belongs to the family of <i>Asfarviridae</i>, part of the group of nucleocytoplasmic large DNA viruses (NCLDV). Little is known about the internalization of ASFV in the host cell and the fusion membrane events that take place at early stages of the infect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/16/3/349 |
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| author | Jesús Urquiza Miguel Ángel Cuesta-Geijo Isabel García-Dorival Óscar Fernández Ana del Puerto José Fernando Díaz Covadonga Alonso |
| author_facet | Jesús Urquiza Miguel Ángel Cuesta-Geijo Isabel García-Dorival Óscar Fernández Ana del Puerto José Fernando Díaz Covadonga Alonso |
| author_sort | Jesús Urquiza |
| collection | DOAJ |
| description | African swine fever virus (ASFV) belongs to the family of <i>Asfarviridae</i>, part of the group of nucleocytoplasmic large DNA viruses (NCLDV). Little is known about the internalization of ASFV in the host cell and the fusion membrane events that take place at early stages of the infection. Poxviruses, also members of the NCLDV and represented by vaccinia virus (VACV), are large, enveloped, double-stranded DNA viruses. Poxviruses were considered unique in having an elaborate entry-fusion complex (EFC) composed of 11 highly conserved proteins integrated into the membrane of mature virions. Recent advances in methodological techniques have again revealed several connections between VACV EFC proteins. In this study, we explored the possibility of an analogous ASFV EFC by identifying ten candidate proteins exhibiting structural similarities with VACV EFC proteins. This could reveal key functions of these ASFV proteins, drawing attention to shared features between the two virus families, suggesting the potential existence of an ASFV entry-fusion complex. |
| first_indexed | 2024-04-24T17:45:47Z |
| format | Article |
| id | doaj.art-193b6c1a04014803a173a849f25fb45a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-24T17:45:47Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-193b6c1a04014803a173a849f25fb45a2024-03-27T14:07:37ZengMDPI AGViruses1999-49152024-02-0116334910.3390/v16030349Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia VirusJesús Urquiza0Miguel Ángel Cuesta-Geijo1Isabel García-Dorival2Óscar Fernández3Ana del Puerto4José Fernando Díaz5Covadonga Alonso6Departamento de Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coruña Km 7.5, 28040 Madrid, SpainDepartamento de Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coruña Km 7.5, 28040 Madrid, SpainDepartamento de Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coruña Km 7.5, 28040 Madrid, SpainUnidad BICS, Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, SpainDepartamento de Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coruña Km 7.5, 28040 Madrid, SpainUnidad BICS, Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, SpainDepartamento de Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coruña Km 7.5, 28040 Madrid, SpainAfrican swine fever virus (ASFV) belongs to the family of <i>Asfarviridae</i>, part of the group of nucleocytoplasmic large DNA viruses (NCLDV). Little is known about the internalization of ASFV in the host cell and the fusion membrane events that take place at early stages of the infection. Poxviruses, also members of the NCLDV and represented by vaccinia virus (VACV), are large, enveloped, double-stranded DNA viruses. Poxviruses were considered unique in having an elaborate entry-fusion complex (EFC) composed of 11 highly conserved proteins integrated into the membrane of mature virions. Recent advances in methodological techniques have again revealed several connections between VACV EFC proteins. In this study, we explored the possibility of an analogous ASFV EFC by identifying ten candidate proteins exhibiting structural similarities with VACV EFC proteins. This could reveal key functions of these ASFV proteins, drawing attention to shared features between the two virus families, suggesting the potential existence of an ASFV entry-fusion complex.https://www.mdpi.com/1999-4915/16/3/349African swine fever virusASFVNCLDVspoxvirusesfusion proteinsentry-fusion complex |
| spellingShingle | Jesús Urquiza Miguel Ángel Cuesta-Geijo Isabel García-Dorival Óscar Fernández Ana del Puerto José Fernando Díaz Covadonga Alonso Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus Viruses African swine fever virus ASFV NCLDVs poxviruses fusion proteins entry-fusion complex |
| title | Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus |
| title_full | Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus |
| title_fullStr | Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus |
| title_full_unstemmed | Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus |
| title_short | Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus |
| title_sort | identification of a potential entry fusion complex based on sequence homology of african swine fever and vaccinia virus |
| topic | African swine fever virus ASFV NCLDVs poxviruses fusion proteins entry-fusion complex |
| url | https://www.mdpi.com/1999-4915/16/3/349 |
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