Functional Dichotomy for a Hyphal Repressor in Candida albicans
ABSTRACT Nrg1 is a repressor of hypha formation and hypha-associated gene expression in the fungal pathogen Candida albicans. It has been well studied in the genetic background of the type strain SC5314. Here, we tested Nrg1 function in four other diverse clinical isolates through an analysis of nrg...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-04-01
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| Series: | mBio |
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| Online Access: | https://journals.asm.org/doi/10.1128/mbio.00134-23 |
| _version_ | 1797839585575698432 |
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| author | Yinhe Mao Norma V. Solis Scott G. Filler Aaron P. Mitchell |
| author_facet | Yinhe Mao Norma V. Solis Scott G. Filler Aaron P. Mitchell |
| author_sort | Yinhe Mao |
| collection | DOAJ |
| description | ABSTRACT Nrg1 is a repressor of hypha formation and hypha-associated gene expression in the fungal pathogen Candida albicans. It has been well studied in the genetic background of the type strain SC5314. Here, we tested Nrg1 function in four other diverse clinical isolates through an analysis of nrg1Δ/Δ mutants, with SC5314 included as a control. In three strains, nrg1Δ/Δ mutants unexpectedly produced aberrant hyphae under inducing conditions, as assayed by microscopic observation and endothelial cell damage. The nrg1Δ/Δ mutant of strain P57055 had the most severe defect. We examined gene expression features under hypha-inducing conditions by RNA-sequencing (RNA-Seq) for the SC5314 and P57055 backgrounds. The SC5314 nrg1Δ/Δ mutant expressed six hypha-associated genes at reduced levels compared with wild-type SC5314. The P57055 nrg1Δ/Δ mutant expressed 17 hypha-associated genes at reduced levels compared with wild-type P57055, including IRF1, RAS2, and ECE1. These findings indicate that Nrg1 has a positive role in hypha-associated gene expression and that this role is magnified in strain P57055. Remarkably, the same hypha-associated genes affected by the nrg1Δ/Δ mutation in strain P57055 were also naturally expressed at lower levels in wild-type P57055 than those in wild-type SC5314. Our results suggest that strain P57055 is defective in a pathway that acts in parallel with Nrg1 to upregulate the expression of several hypha-associated genes. IMPORTANCE Hypha formation is a central virulence trait of the fungal pathogen Candida albicans. Control of hypha formation has been studied in detail in the type strain but not in other diverse C. albicans clinical isolates. Here, we show that the hyphal repressor Nrg1 has an unexpected positive role in hypha formation and hypha-associated gene expression, as revealed by the sensitized P57055 strain background. Our findings indicate that reliance on a single type strain limits understanding of gene function and illustrate that strain diversity is a valuable resource for C. albicans molecular genetic analysis. |
| first_indexed | 2024-04-09T16:00:21Z |
| format | Article |
| id | doaj.art-193b9cb1b897468d99554e31cd075ed8 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-04-09T16:00:21Z |
| publishDate | 2023-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-193b9cb1b897468d99554e31cd075ed82023-04-25T13:04:57ZengAmerican Society for MicrobiologymBio2150-75112023-04-0114210.1128/mbio.00134-23Functional Dichotomy for a Hyphal Repressor in Candida albicansYinhe Mao0Norma V. Solis1Scott G. Filler2Aaron P. Mitchell3Department of Microbiology, University of Georgia, Athens, Georgia, USALundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, California, USALundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, California, USADepartment of Microbiology, University of Georgia, Athens, Georgia, USAABSTRACT Nrg1 is a repressor of hypha formation and hypha-associated gene expression in the fungal pathogen Candida albicans. It has been well studied in the genetic background of the type strain SC5314. Here, we tested Nrg1 function in four other diverse clinical isolates through an analysis of nrg1Δ/Δ mutants, with SC5314 included as a control. In three strains, nrg1Δ/Δ mutants unexpectedly produced aberrant hyphae under inducing conditions, as assayed by microscopic observation and endothelial cell damage. The nrg1Δ/Δ mutant of strain P57055 had the most severe defect. We examined gene expression features under hypha-inducing conditions by RNA-sequencing (RNA-Seq) for the SC5314 and P57055 backgrounds. The SC5314 nrg1Δ/Δ mutant expressed six hypha-associated genes at reduced levels compared with wild-type SC5314. The P57055 nrg1Δ/Δ mutant expressed 17 hypha-associated genes at reduced levels compared with wild-type P57055, including IRF1, RAS2, and ECE1. These findings indicate that Nrg1 has a positive role in hypha-associated gene expression and that this role is magnified in strain P57055. Remarkably, the same hypha-associated genes affected by the nrg1Δ/Δ mutation in strain P57055 were also naturally expressed at lower levels in wild-type P57055 than those in wild-type SC5314. Our results suggest that strain P57055 is defective in a pathway that acts in parallel with Nrg1 to upregulate the expression of several hypha-associated genes. IMPORTANCE Hypha formation is a central virulence trait of the fungal pathogen Candida albicans. Control of hypha formation has been studied in detail in the type strain but not in other diverse C. albicans clinical isolates. Here, we show that the hyphal repressor Nrg1 has an unexpected positive role in hypha formation and hypha-associated gene expression, as revealed by the sensitized P57055 strain background. Our findings indicate that reliance on a single type strain limits understanding of gene function and illustrate that strain diversity is a valuable resource for C. albicans molecular genetic analysis.https://journals.asm.org/doi/10.1128/mbio.00134-23Candida albicansgene regulationgeneticshyphal developmentnatural variation |
| spellingShingle | Yinhe Mao Norma V. Solis Scott G. Filler Aaron P. Mitchell Functional Dichotomy for a Hyphal Repressor in Candida albicans mBio Candida albicans gene regulation genetics hyphal development natural variation |
| title | Functional Dichotomy for a Hyphal Repressor in Candida albicans |
| title_full | Functional Dichotomy for a Hyphal Repressor in Candida albicans |
| title_fullStr | Functional Dichotomy for a Hyphal Repressor in Candida albicans |
| title_full_unstemmed | Functional Dichotomy for a Hyphal Repressor in Candida albicans |
| title_short | Functional Dichotomy for a Hyphal Repressor in Candida albicans |
| title_sort | functional dichotomy for a hyphal repressor in candida albicans |
| topic | Candida albicans gene regulation genetics hyphal development natural variation |
| url | https://journals.asm.org/doi/10.1128/mbio.00134-23 |
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