Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction
The vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding the biochemical mechanisms involved in resistance to Fod will allow the development of new disease control strategies. In this research, the levels of some phy...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Floricultura e Plantas Ornamentais
2021-10-01
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| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://www.ornamentalhorticulture.com.br/rbho/article/view/2412 |
| _version_ | 1797831307767578624 |
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| author | Leidy Johana Vanegas-Cano Sixta Tulia Martínez-Peralta Ericsson Coy-Barrera Harold Duban Ardila-Barrantes |
| author_facet | Leidy Johana Vanegas-Cano Sixta Tulia Martínez-Peralta Ericsson Coy-Barrera Harold Duban Ardila-Barrantes |
| author_sort | Leidy Johana Vanegas-Cano |
| collection | DOAJ |
| description | The vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding the biochemical mechanisms involved in resistance to Fod will allow the development of new disease control strategies. In this research, the levels of some phytohormones such as salicylic acid (SA), methyl salicylate (MeSA), and methyl jasmonate (MeJA) were evaluated in symplast of carnation roots infected with this pathogen. The accumulation of these hormones was then correlated with the expression levels of symplastic peroxidases, enzymes involved in the plant resistance against pathogen during interaction. Our results suggested that pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’). Simultaneously, an increase of guaiacol peroxidase enzymatic activity (GPX) and transcriptional levels of a gene coding for a symplastic peroxidase were presented as part of the resistance response. The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels) indicates the possible cellular relationship of these phenomena during the activation of the resistance to Fod. Our findings suggested some hormonal signaling mechanisms acting at the roots during the regulation of the biochemical response associated with resistance against Fod. |
| first_indexed | 2024-04-09T13:50:55Z |
| format | Article |
| id | doaj.art-1940eea81e6a4c2ba1ca0d8df0c04091 |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-04-09T13:50:55Z |
| publishDate | 2021-10-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-1940eea81e6a4c2ba1ca0d8df0c040912023-05-08T17:59:04ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2021-10-0128110.1590/2447-536X.v28i1.2412Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interactionLeidy Johana Vanegas-Cano0Sixta Tulia Martínez-Peralta1Ericsson Coy-Barrera2Harold Duban Ardila-Barrantes3Universidad Nacional de ColombiaUniversidad Nacional de ColombiaUniversidad Militar Nueva GranadaUniversidad Nacional de ColombiaThe vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding the biochemical mechanisms involved in resistance to Fod will allow the development of new disease control strategies. In this research, the levels of some phytohormones such as salicylic acid (SA), methyl salicylate (MeSA), and methyl jasmonate (MeJA) were evaluated in symplast of carnation roots infected with this pathogen. The accumulation of these hormones was then correlated with the expression levels of symplastic peroxidases, enzymes involved in the plant resistance against pathogen during interaction. Our results suggested that pathogen infection causes a differential accumulation of SA, MeSA, and MeJA in a resistant cultivar (i.e. ‘Golem’), being earlier and higher than that observed in a susceptible one (i.e. ‘Solex’). Simultaneously, an increase of guaiacol peroxidase enzymatic activity (GPX) and transcriptional levels of a gene coding for a symplastic peroxidase were presented as part of the resistance response. The positive statistical correlation between the accumulation of SA and MeJA and the expression of peroxidases (GPX activity and mRNA levels) indicates the possible cellular relationship of these phenomena during the activation of the resistance to Fod. Our findings suggested some hormonal signaling mechanisms acting at the roots during the regulation of the biochemical response associated with resistance against Fod.https://www.ornamentalhorticulture.com.br/rbho/article/view/2412guaiacol peroxidasemethyl jasmonatemethyl salicylatesalicylic acid |
| spellingShingle | Leidy Johana Vanegas-Cano Sixta Tulia Martínez-Peralta Ericsson Coy-Barrera Harold Duban Ardila-Barrantes Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction Ornamental Horticulture guaiacol peroxidase methyl jasmonate methyl salicylate salicylic acid |
| title | Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction |
| title_full | Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction |
| title_fullStr | Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction |
| title_full_unstemmed | Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction |
| title_short | Plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation-Fusarium oxysporum interaction |
| title_sort | plant hormones accumulation and its relationship with symplastic peroxidases expression during carnation fusarium oxysporum interaction |
| topic | guaiacol peroxidase methyl jasmonate methyl salicylate salicylic acid |
| url | https://www.ornamentalhorticulture.com.br/rbho/article/view/2412 |
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