<i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp.
Severe ocular infections by <i>Acanthamoeba</i> sp. lead to keratitis, resulting in irreversible vision loss in immune-compromised individuals. When a protozoal infection spreads to neural tissues, it causes granulomatous encephalitis, which can be fatal. Treatment often takes longer due...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/12/2502 |
| _version_ | 1797456128395706368 |
|---|---|
| author | Pradnya B. Nikam Jitendra D. Salunkhe Kiran R. Marathe Mousa A. Alghuthaymi Kamel A. Abd-Elsalam Satish V. Patil |
| author_facet | Pradnya B. Nikam Jitendra D. Salunkhe Kiran R. Marathe Mousa A. Alghuthaymi Kamel A. Abd-Elsalam Satish V. Patil |
| author_sort | Pradnya B. Nikam |
| collection | DOAJ |
| description | Severe ocular infections by <i>Acanthamoeba</i> sp. lead to keratitis, resulting in irreversible vision loss in immune-compromised individuals. When a protozoal infection spreads to neural tissues, it causes granulomatous encephalitis, which can be fatal. Treatment often takes longer due to the transition of amoeba from trophozoites to cyst stages, cyst being the dormant form of <i>Acanthamoeba</i>. A prolonged use of therapeutic agents, such as ciprofloxacin (Cipro), results in severe side effects; thus, it is critical to improve the therapeutic efficacy of these widely used antibiotics, possibly by limiting the drug-sensitive protozoal-phase transition to cyst formation. Owing to the biomedical potential of selenium nanoparticles (SeNPs), we evaluated the synergistic effects of ciprofloxacin and <i>Rhizobium pusense</i>–biogenic SeNPs combination. SeNPs synthesized using <i>Rhizobium pusense</i> isolated from root nodules were characterized using UV–Visible spectrophotometer, FT-IR, SEM with EDX, particle size analysis, and Zeta potential. The combination was observed to reduce the sub-lethal dose of Cipro, which may help reduce its side effects. The selenium and ciprofloxacin (SeNPs–Cipro) combination reduced the LC<sub>50</sub> by 33.43%. The anti-protozoal efficacy of SeNPs–Cipro was found to transduce through decreased protozoal-cyst formations and the inhibition of the galactosidase and protease enzymes of trophozoites. Furthermore, high leakage of sugar, proteins, and amino acids during the SeNPs–Cipro treatment was one primary reason for killing the trophozoites. These experimental results may be helpful in the further pre-clinical evaluation of SeNPs–Cipro to combat protozoal infections. Future studies for combinations of SeNPs with other antibiotics need to be conducted to know the potential of SeNPs against antibiotic resistance in <i>Acanthamoeba.</i> |
| first_indexed | 2024-03-09T16:03:55Z |
| format | Article |
| id | doaj.art-19423678d8874146b1281b210bfc8d49 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:03:55Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-19423678d8874146b1281b210bfc8d492023-11-24T16:50:11ZengMDPI AGMicroorganisms2076-26072022-12-011012250210.3390/microorganisms10122502<i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp.Pradnya B. Nikam0Jitendra D. Salunkhe1Kiran R. Marathe2Mousa A. Alghuthaymi3Kamel A. Abd-Elsalam4Satish V. Patil5Department of Biochemistry, School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon 425001, IndiaDepartment of Biochemistry, School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon 425001, IndiaDepartment of Biochemistry, School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon 425001, IndiaBiology Department, Science and Humanities College, Shaqra University, Alquwayiyah 11971, Saudi ArabiaPlant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptDepartment of Biochemistry, School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon 425001, IndiaSevere ocular infections by <i>Acanthamoeba</i> sp. lead to keratitis, resulting in irreversible vision loss in immune-compromised individuals. When a protozoal infection spreads to neural tissues, it causes granulomatous encephalitis, which can be fatal. Treatment often takes longer due to the transition of amoeba from trophozoites to cyst stages, cyst being the dormant form of <i>Acanthamoeba</i>. A prolonged use of therapeutic agents, such as ciprofloxacin (Cipro), results in severe side effects; thus, it is critical to improve the therapeutic efficacy of these widely used antibiotics, possibly by limiting the drug-sensitive protozoal-phase transition to cyst formation. Owing to the biomedical potential of selenium nanoparticles (SeNPs), we evaluated the synergistic effects of ciprofloxacin and <i>Rhizobium pusense</i>–biogenic SeNPs combination. SeNPs synthesized using <i>Rhizobium pusense</i> isolated from root nodules were characterized using UV–Visible spectrophotometer, FT-IR, SEM with EDX, particle size analysis, and Zeta potential. The combination was observed to reduce the sub-lethal dose of Cipro, which may help reduce its side effects. The selenium and ciprofloxacin (SeNPs–Cipro) combination reduced the LC<sub>50</sub> by 33.43%. The anti-protozoal efficacy of SeNPs–Cipro was found to transduce through decreased protozoal-cyst formations and the inhibition of the galactosidase and protease enzymes of trophozoites. Furthermore, high leakage of sugar, proteins, and amino acids during the SeNPs–Cipro treatment was one primary reason for killing the trophozoites. These experimental results may be helpful in the further pre-clinical evaluation of SeNPs–Cipro to combat protozoal infections. Future studies for combinations of SeNPs with other antibiotics need to be conducted to know the potential of SeNPs against antibiotic resistance in <i>Acanthamoeba.</i>https://www.mdpi.com/2076-2607/10/12/2502<i>Acanthamoeba</i>nano-seleniumtrophozoitescystsciprofloxacinanti-protozoal |
| spellingShingle | Pradnya B. Nikam Jitendra D. Salunkhe Kiran R. Marathe Mousa A. Alghuthaymi Kamel A. Abd-Elsalam Satish V. Patil <i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp. Microorganisms <i>Acanthamoeba</i> nano-selenium trophozoites cysts ciprofloxacin anti-protozoal |
| title | <i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp. |
| title_full | <i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp. |
| title_fullStr | <i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp. |
| title_full_unstemmed | <i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp. |
| title_short | <i>Rhizobium pusense-</i>Mediated Selenium Nanoparticles–Antibiotics Combinations against <i>Acanthamoeba</i> sp. |
| title_sort | i rhizobium pusense i mediated selenium nanoparticles antibiotics combinations against i acanthamoeba i sp |
| topic | <i>Acanthamoeba</i> nano-selenium trophozoites cysts ciprofloxacin anti-protozoal |
| url | https://www.mdpi.com/2076-2607/10/12/2502 |
| work_keys_str_mv | AT pradnyabnikam irhizobiumpusenseimediatedseleniumnanoparticlesantibioticscombinationsagainstiacanthamoebaisp AT jitendradsalunkhe irhizobiumpusenseimediatedseleniumnanoparticlesantibioticscombinationsagainstiacanthamoebaisp AT kiranrmarathe irhizobiumpusenseimediatedseleniumnanoparticlesantibioticscombinationsagainstiacanthamoebaisp AT mousaaalghuthaymi irhizobiumpusenseimediatedseleniumnanoparticlesantibioticscombinationsagainstiacanthamoebaisp AT kamelaabdelsalam irhizobiumpusenseimediatedseleniumnanoparticlesantibioticscombinationsagainstiacanthamoebaisp AT satishvpatil irhizobiumpusenseimediatedseleniumnanoparticlesantibioticscombinationsagainstiacanthamoebaisp |