Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i>
<i>Selaginella sinensis</i> (Desv.) Spring has been used for many years as traditional Chinese medicine (TCM) for many years. Recently, ionic liquids (ILs) have attracted great attentions in extraction and separation technology of TCM as a new green solvent. In this paper, microwave assi...
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MDPI AG
2019-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/13/2507 |
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| author | Dan Li Chengxin Sun Jiaqiang Yang Xiankui Ma Yongmei Jiang Shaoliang Qiu Gang Wang |
| author_facet | Dan Li Chengxin Sun Jiaqiang Yang Xiankui Ma Yongmei Jiang Shaoliang Qiu Gang Wang |
| author_sort | Dan Li |
| collection | DOAJ |
| description | <i>Selaginella sinensis</i> (Desv.) Spring has been used for many years as traditional Chinese medicine (TCM) for many years. Recently, ionic liquids (ILs) have attracted great attentions in extraction and separation technology of TCM as a new green solvent. In this paper, microwave assisted extraction-IL (MAE-IL) that extracted amentoflavone (AME) and hinokiflavone (HIN) from <i>Selaginella sinensis</i> was reported for the first time. The contents of two biflavonoids were simultaneously determined by a high performance liquid chromatography (HPLC) method. After different ionic liquids were compared, it was found [C<sub>6</sub>mim]BF<sub>4</sub> had a high selectivity and efficiency. Moreover, the important extraction conditions, including solid-liquid ratio, IL concentration, extraction time, microwave power and radiation temperature, were also investigated and optimized by response surface methodology (RSM) using AME and HIN yields as index. The results showed that the extraction yields of AME and HIN from <i>S. sinensis</i> were 1.96 mg/g and 0.79 mg/g, respectively, under the optimal process parameters (0.55 mmol/L, 300 W, 40 min, 1:11 g/mL and 48 °C). Compared with the conventional extraction methods, MAE-IL could not only achieve higher yield in shorter time, but also could reduce the consumption of solvent. This effective, rapid and green MAE-IL method was suitable for the extraction of AME and HIN. |
| first_indexed | 2024-12-18T19:45:58Z |
| format | Article |
| id | doaj.art-1945e1dbff9e4643886057b73ef8cb4e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-18T19:45:58Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1945e1dbff9e4643886057b73ef8cb4e2022-12-21T20:55:18ZengMDPI AGMolecules1420-30492019-07-012413250710.3390/molecules24132507molecules24132507Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i>Dan Li0Chengxin Sun1Jiaqiang Yang2Xiankui Ma3Yongmei Jiang4Shaoliang Qiu5Gang Wang6School of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, ChinaSchool of Pharmacy, Zunyi Medical University, Zunyi, Guizhou 563003, China<i>Selaginella sinensis</i> (Desv.) Spring has been used for many years as traditional Chinese medicine (TCM) for many years. Recently, ionic liquids (ILs) have attracted great attentions in extraction and separation technology of TCM as a new green solvent. In this paper, microwave assisted extraction-IL (MAE-IL) that extracted amentoflavone (AME) and hinokiflavone (HIN) from <i>Selaginella sinensis</i> was reported for the first time. The contents of two biflavonoids were simultaneously determined by a high performance liquid chromatography (HPLC) method. After different ionic liquids were compared, it was found [C<sub>6</sub>mim]BF<sub>4</sub> had a high selectivity and efficiency. Moreover, the important extraction conditions, including solid-liquid ratio, IL concentration, extraction time, microwave power and radiation temperature, were also investigated and optimized by response surface methodology (RSM) using AME and HIN yields as index. The results showed that the extraction yields of AME and HIN from <i>S. sinensis</i> were 1.96 mg/g and 0.79 mg/g, respectively, under the optimal process parameters (0.55 mmol/L, 300 W, 40 min, 1:11 g/mL and 48 °C). Compared with the conventional extraction methods, MAE-IL could not only achieve higher yield in shorter time, but also could reduce the consumption of solvent. This effective, rapid and green MAE-IL method was suitable for the extraction of AME and HIN.https://www.mdpi.com/1420-3049/24/13/2507<i>Selaginella sinensis</i>ionic liquidsamentoflavonehinokiflavonemicrowave extractionHPLCresponse surface methodology |
| spellingShingle | Dan Li Chengxin Sun Jiaqiang Yang Xiankui Ma Yongmei Jiang Shaoliang Qiu Gang Wang Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i> Molecules <i>Selaginella sinensis</i> ionic liquids amentoflavone hinokiflavone microwave extraction HPLC response surface methodology |
| title | Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i> |
| title_full | Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i> |
| title_fullStr | Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i> |
| title_full_unstemmed | Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i> |
| title_short | Ionic Liquid-Microwave-Based Extraction of Biflavonoids from <i>Selaginella sinensis</i> |
| title_sort | ionic liquid microwave based extraction of biflavonoids from i selaginella sinensis i |
| topic | <i>Selaginella sinensis</i> ionic liquids amentoflavone hinokiflavone microwave extraction HPLC response surface methodology |
| url | https://www.mdpi.com/1420-3049/24/13/2507 |
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